Muscimol ingestion method

[rOm]

- Would muscimol would be easily digested and orally active efficiently at 10 mg ?
- Is there any preferred method of administration such as vaporized or sublingual ?

 

[rOm]

it looks now that vaporization is good with it. I just need to find out the vaporization point.

 

[delta-9]

Where SWIM live annually there is a massive fly agaric flush, literally hundreds of caps pop up over the course of a few weeks usually directly before libs come in. What I want to know is has anyone posted an extraction tek for muscimol from the mushrooms, because SWIMfound eating them disgusting and they had a quite nasty poisonous feeling. An extract in gel caps could be the answer. Thanks

 

[jamie]

apparently its very very hard to extract muscimol. If you can do it you can make a living of it..Johnothan ott did for a while.

 

[rOm]

The problem here is to get consistant effects from muscimol. I never got upset with these mushrooms.
There are many way to prepair the collected mushroom Delta-9, how did you cook them beforehand ?

 

[delta-9]

Baked in oven for about 2 hours and 140c, this may not have been hot enough. Also swim picked them when it was a rainy day so they obviously would have taken longer to dry. Perhaps SWIM just got it wrong, SWIM will try again this year.

 

[rOm]

Why not 100°C ? And were you baking fresh mushrooms ? If so did you tried to just lightly bake them and collect only the juice, juice that can be drunk as is or dried for later use (eventually gelcaped).

 

[expanding]

Bit offtopic but is muscimol a worthwhile experience? Iv tryed dried fly agarics a couple of times and got nothing atall from them, but maybe i wasnt taking enough. What kind of effects do you expect? how powerfull is the experience likely to be?

 

[rOm]

Thing is you may have some profound and highly entheogenic experience. But not consistant. Synergistic plant as far as I can tell. But my success experiment lead me to highly estimate its potential.

My guess is working with muscimol might keep it more easy to evaluate. Just hoping it will not be failure...

Vaporization is possible at 175-200° C (347 to 392°F).

A few mg are enough.

 

[delta-9]

Why not 100°C ? And were you baking fresh mushrooms ? If so did you tried to just lightly bake them and collect only the juice, juice that can be drunk as is or dried for later use (eventually gelcaped).

So in the future I should bake the fresh agarics in oven at 100c and collect the juice which can be drunk. What should I do with the bulk part of the mushroom, bin it?

 

[rOm]

Some report nausea with the bulk when the juice has been recolted.
The juice should be the best part.

If it your case, I would suggest to give them back to earth as a compost

Note that I am not working with fresh amanitas this year, and am still researching how to get the most muscimol working without side effects. Will need to invest time this automn for the fresh fruit.

 

[SnozzleBerry]

Something I found at the Shroomery...any thoughts?

WIRKSTOFFE AUS AMANITA MUSCARIA :
IBOTENSAEURE UND MUSCAZON

Die allgemein bekannten Wirkstoffe des Fliegenpilzes,
wie Muscarin, Acetycholin und Muscaridin bieten keine
Erklärung für das Gesamtbild der Fliegenpilzvergiftung
(1). Wir haben deshalb schon vor längerer Zeit nach
weiteren, physiologisch aktiven inhaltsstoffen zu suchen
begonnen. Eine breite pharmakologische Prüfung der
wasserlöslichen Anteile ergab das Vorliegen von
Fraktionen mit starker narkosepotenzierender Wirkung.
Zur präparativen Isolierung diente ein entfetteter
Alkoholextrakt aus Amanita muscaria, der besonders
schonend aufgearbeitet worden war. Durch sorgfältige
Trennung an Dowex 50 W x 12 (H) mit wasser und
verdünnter Ameisensäure erhielten wir kristallisierte
Ibotensäure (2) Smp. 145 (Zers.) , C5H8N2O5 und
Muscazon, Smp. > 190 (Zers.) , C5H6N2O4 . Beide
Verbindungen reagieren mit Ninhydrin auf Papier gelb
und geben mit Grote’s reagens blaue Flecken.

Die Ausbeute an Ibotensäure betrug in der Ernte 196?
215g aus ca. 700 kg Frischpilzen, d.h. 0,03 %. Der
wirkliche Gehalt darf auf 0,05 % geschätzt werden,
sodass Ibotensäure wegen ihrer hohen
pharmakodynamischen wirksamkeit als wesentlicher
inhaltsstoff von Amanita muscaria angesehen werden
darf.

Nach weniger schonender Aufarbeitung isolierten wir
196? einen Wirkstoff , C4H6N2O2, Smp. 174-175 , aus
wasser - methanol, welcher sich als Abbauprodukt von
Ibotensäure erwies.




ACTIVE SUBSTANCES FROM AMANITA
MUSCARIA: IBOTENSAEURE AND MUSCAZON
Those generally did not admit active substances the fly
mushroom, like Muscarin, Acetycholin and Muscaridin
offer an explanation for the overall view of the fly
mushroom poisoning (1). We therefore already began to
look before longer time for further, physiologically active
contents materials. A broad pharmakologische
examination of the water-soluble portions resulted in
being present parliamentary groups with strong
narkosepotenzierender effect. For the praeparativen
isolation a degreased alcohol excerpt from Amanita
served muscaria, which had been particularly carefully
regenerated. By careful separation at Dowex 50 W x 12
(H) with water and diluted formic acid we received
crystallized Ibotensaeure (2) Smp. 145 (Zers.) ,
C5H8N2O5 and Muscazon, Smp. 190 (Zers.) ,
C5H6N2O4. Both connections react with Ninhydrin to
paper yellow and give with Grote's reagent blue marks.
The yield at Ibotensaeure amounted to in the harvest
196? 215g out approx.. 700 kg fresh mushrooms, i.e.
0.03 %. the real content may become estimated on 0,05
%, so that Ibotensaeure may be regarded because of their
high pharmacodynamic effectiveness as substantial
contents material of Amanita muscaria. After less careful
processing we isolated 196? an active substance,
C4H6N2O2, Smp. 174-175, from water - methanol,
which proved as dismantling product of Ibotensaeure.



paper 2:


A novel constituent of amanita muscaria


isolation of 3-hydroxyisoxazole from natural sources.

fresh plants were added to boiling water, cooled rapidly,
filtered and the filtrates treated with Deacidite FF (base)
resin. The resin was washed with water and the
compound eluted by means of acetic acid.The eluate was
freeze-dried, disolved in water and passed down a
column of celulose phosphate (whatman P70). the
substance gave a pale yellow band which was elated with
N NH4OH and the eluate freeze dried . The
semi-purified material was crystallized from alcohol to
give the compound, mp. 172-174 (decomp)

 

[rOm]

SO Snozzleberry, this would be in substance a tek giving us a TAE (the resinous substance we've came across) ?
This might need a trial.

Is deacidifate FF (base) resin means Ion exchange resin ?
ANd which solvent is N NH4OH ?
We could start form there but simplify the tek a bit for kitchen chemist (the step of passing through a colmumn of cellulose phosphate could be changed).

 

[rOm]

Also to go deeper in muscimol extraction, I reckon I need to put my hand on some chromatographic equipments.
Sounds exciting !!
I'll wait the fall to go around pick some material to work with I guess. Unless I get impatient and buy
a bunch of them =)

 

[rOm]

Next bioessay:

Transdermal muscimol

 

[rOm]

When Chromotography will be at hand, I found a paper to distinct the different compounds of interest :

Abstract
OBJECTIVES: Ibotenic acid, muscimol and muscarine were recognized as responsible for psychotropic effects of A. muscaria and A. pantherina. Demand for their specific and sensitive identification and quantitation in biological material lead to effort to develop reliable analytical method, but satisfactory solution is still lacking. Presented article describes liquid chromatography-mass spectrometry method suitable for isolation and identification of principal toxins of A. muscaria and A. pantherina in urine. METHODS AND RESULTS: Dedicated liquid chromatography-mass spectrometry method is reported. Technique consists of an extraction of analytes on Strata X-CW and Discovery SCX SPE cartridges and separation is achieved using a Gemini C18 column (150 mm x 2.0 mm, 5 micron) and 8 mM heptafluorobutyric acid as mobile phase. Detection at m/z 159 for ibotenic acid, m/z 115 for muscimol and m/z 174 for muscarine was used. Retention times and LODs are 2.6 min and 50 ng.ml-1 for ibotenic acid, 4.6 min and 40 ng.ml-1 for muscimol and 14.2 min and 3 ng.ml-1 for muscarine. CONCLUSION: A sensitive and specific liquid chromatography-mass spectrometry assay was developed for analysis of principal toxins of A. muscaria and A. pantherina in urine. Method was found to be sufficiently sensitive and specific for analysis of urine of intoxicated patients.

 

[Parshvik Chintan]

Next bioessay:

Transdermal muscimol

so uh... did anything ever happen with this?

 

[rOm]

Not really. Missed the oportunity to get all the material and try make some work out of it.
But this slicky compound is definitely interesting !

 

[Parshvik Chintan]

page 41 of this book discusses the preparation of a topical oil made out of muscaria

Preparing the Holy Oil
Olive oil and animal fat are both absorbed into the skin. As far as preservability
goes, olive oil has an edge over animal fat, especially in warm or hot conditions.
The active principal of the herb can also be absorbed into the skin; if you gently
hold a fresh Amanita Muscaria stem in your hand for about 5 minutes, you will
start feeling a light giddiness, the longer the contact, the more intense the effects. [this claim seems a more than a bit dubious]

To create the holy oil, powder some dried specimens and sift the powder
through a fine cloth. Return the pieces that didn’t pass to being repowdered. Once
you have the desired amount of fine herb powder, place into a clean dry small
sealable vial or bottle. Slowly add just enough olive oil to cover the powder.
Using a piece of wire, gently stir the powder and olive oil together. Add more oil
as needed to create a thin paste. Seal the container and let the herb soak in the oil
for an hour or two. To use, place a fingertip on the surface of the oil and apply to
the center of the forehead about an inch or so above the eyebrows. The effects
should start in a few minutes and last until the oil is all absorbed. This is the most
controllable method of using dried Amanita Muscaria and probably the most
efficient way of using a limited quantity of dried mushroom for a number of
people. The effects are never too intense unless too much oil was applied

This oil is also a very effective topical antibiotic and can be used on many
different infections of the skin; such as inflamed insect bites or other infected
wounds.

my question is: is the oil necessary?

if i do a crude extract on amanita, could that be placed directly on my skin? or is the olive oil a necessary aid for absorption?

 

[Parshvik Chintan]

administered a liberal amount of crude amanita extract (already confirmed for activity) topically (mostly on the forehead as per the above passage - also on neck and inner elbow joints)

so far its been ~30 min or so. no effects noted as of yet.

EDIT:
~60 min. mild effects, similar to about the same amount consumed orally - with no ill effects. visual perception altered slightly. very mild and enjoyable. slight drowsiness. bit of an uplifted mood (i wouldn't go so far as to say euphoria) and i feel a bit chatty.