downwardsfromzero
Boundary condition
mycosis - one thing to try would be making some calcium fumarate on purpose and testing its solubility in acetone and water. It may be necessary to precipitate it from limonene as before in order to account for possible polymorphs.
Testing with a suitable reagent kit would help confirm the presence of alkaloid.
It's possible that the fumaric acid has precipitated out of the FASI on addition to the limonene. This may be ameliorated to an extent - next time - by spiking the dried limonene with dry IPA before adding the FASI. Anhydrous sodium carbonate seems to work for drying limonene.
Testing with a suitable reagent kit would help confirm the presence of alkaloid.
This strongly suggests it is at least very heavily contaminated with, if not entirely, fumaric acid or/and calcium fumarate. But then I don't actually know the solubility of 5-MeO fumarate (if anyone even does!)it took ~200 ml of water to dissolve 150mg of this stuff...is that normal?
It's possible that the fumaric acid has precipitated out of the FASI on addition to the limonene. This may be ameliorated to an extent - next time - by spiking the dried limonene with dry IPA before adding the FASI. Anhydrous sodium carbonate seems to work for drying limonene.