Thanks benzyme, another interesting paper. After reading it, I wonder if we can use Raman to check for HPBCD complexation also. We have the drinkable/sublingual/nebulizable complexed salvinorin project and monitoring complexation rates would be very helpful.

One question about the calibration. As I understand it, adjusting the optics centers the wavelength of interest and focuses (narrows) the peaks. The first mirror needs to align with the slit/fiber and have it in its focal point. The second mirror needs to have the CCD detector in its focal "plane". The diffraction grid receives and emits collimated light and the incident angle on the grid determines what wavelenghts get sent to the CCD detector.

After that, to calibrate the pixel number to a wavelenth (or ramam shift), the program needs to fit a 3rd order polynomial to a know reference spectrum, right? What polynomial coefficients did you get? What did you use as a reference?

Thanks again.