digitalvygr
Rising Star
Thanks for attaching the article! Here is what I took away:
They synthesized a tryptamine derivative called propyl dimethyl amino tryptamine (PDAT).
PDAT was able to inhibit INMT selectively, i.e. it did not inhibit 2 other methyltransferases they tested.
This in turn indicated the presence of an inhibitory allosteric site, so they used in silico computer modelling to find out where that site might be on the INMT molecule. They found a site that looks like can dock both PDAT and DMT which would explain the physical mechanism behind why DMT itself inhibits INMT as does the PDAT.
My take is that this could be the mechanism behind how the "small protein" or other substance from the original studies was inhibiting INMT. (Given that DMT itself proved inhibitory I wonder if it even had to be a "small protein" or rather just a "small molecule"). I do recall though reading *somewhere* though that they had measured its molecular weight indirectly somehow and said it was a peptide.)
Out of the 60 or so references, one that might be worth following for a similar allosteric inhibitory process is this paper describing inhibition of protein arginine methyltransferase 3 (PRMT3):
www.ncbi.nlm.nih.gov
They synthesized a tryptamine derivative called propyl dimethyl amino tryptamine (PDAT).
PDAT was able to inhibit INMT selectively, i.e. it did not inhibit 2 other methyltransferases they tested.
This in turn indicated the presence of an inhibitory allosteric site, so they used in silico computer modelling to find out where that site might be on the INMT molecule. They found a site that looks like can dock both PDAT and DMT which would explain the physical mechanism behind why DMT itself inhibits INMT as does the PDAT.
My take is that this could be the mechanism behind how the "small protein" or other substance from the original studies was inhibiting INMT. (Given that DMT itself proved inhibitory I wonder if it even had to be a "small protein" or rather just a "small molecule"). I do recall though reading *somewhere* though that they had measured its molecular weight indirectly somehow and said it was a peptide.)
Out of the 60 or so references, one that might be worth following for a similar allosteric inhibitory process is this paper describing inhibition of protein arginine methyltransferase 3 (PRMT3):
An allosteric inhibitor of protein arginine methyltransferase 3 - PubMed
PRMT3, a protein arginine methyltransferase, has been shown to influence ribosomal biosynthesis by catalyzing the dimethylation of the 40S ribosomal protein S2. Although PRMT3 has been reported to be a cytosolic protein, it has been shown to methylate histone H4 peptide (H4 1-24) in vitro. Here...
www.ncbi.nlm.nih.gov
