If you're not getting a 4 to 5 hour fully orally active effect from oral DMT, you're either not using enough Harmalas, or the timing between the Harmalas and the DMT needs to be explored/tinkered with. I've taken as low as 1 to 3 grams of Mimosa root powder encapsulated, and with proper MAO-A inhibition going on when the DMT is orally consumed, you should get full oral activation. Granted, the dosage of DMT may also be a little bit of a factor, since if you don't take enough you may find yourself coming up but then things kinda just "drop off" but there should still be some effects for the full duration of 4 to 5 hours, ime, 3 hours at the least, anything less than 3 to 5 hours total for oral DMT means it's not being fully orally activated.
As i like to say, the more Harmalas you use, the less DMT you'll need, the less Harmalas you use, the more DMT you'll need, but the less Harmalas you use or if you don't get the timing right, the shorter the duration will be. I've had oral DMT come up and then quickly leave my system completely within like 30 minutes to an hour, due to improper Harmala dosage/timing.
Also, i'm very experienced with Rue, i've taken it pretty much daily/near daily since March 2012, in heavy dosages for the most part, with some breaks here and there over the years, and 1 gram of Rue definitely isn't enough, i wouldn't recommend less than 2.5 grams, not even 2 grams, it just isn't enough, but ideally i always go for and recommend the usual/average/common dosage range of 3 to 4.5 grams. And as for timing, i usually recommend 30 to 45 minutes to an hour between the Harmalas and the oral DMT, preferably an hour (particularly if in tea form) as an hour apart seems to do me best.
The only way i can see Rue dosages of 1 to 2 grams being sufficient, is in the rare case that someone is extremely low in the CYP2D6 liver enzyme (which metabolizes the Harmalas) or possibly they are already quite low in MAO-A, however i'd bet on the CYP2D6 enzyme personally, but in most cases (unless one also is really super sensitive to other CYP2D6 substrates), 1 to 2 grams will not be enough, i'm willing to bet that even with low CYP2D6 status that 1 gram wouldn't be enough, 2 grams maybe, but 2.5 grams possibly, but even then i doubt 1 to 2 grams is going to do much of anything for most people, like 99.5% of people quite possibly, even 2.5 to 3 grams for most people isn't going to take them as deeply as a higher dosage, but 3 to 4.5 grams seems to be where it's at for the most part.
I've also noticed that, because i take Harmalas/Rue regularly, it will build up a reverse tolerance which makes each dose, at the same dosage, stronger and stronger the more i take it, which as time goes on takes me into heavy Harmala territory without needing to up the dosage of Rue, also the side-effects will clean up/go away over time, so no more nausea/vomiting/possible diarrhea, the bodyload and headspace is cleaned up, and the motor function impairment is reduced, it overall feels cleaner, clearer, and more medicinal, and a lot more manageable even at heavy dosages. Anyways, as for the reverse tolerance, personally ime i've noticed a few things about it, firstly, that if i go down to 2.5 grams of Rue, it seems to be enough to build up the reverse tolerance or keep it going, to some degree, but below 2.5 grams, like 2 grams, the reverse tolerance isn't there, so there seems to be a level you hit in dosage where the reverse tolerance starts kicking into effect. Secondly, i've noticed that the more Rue/Harmalas you take, the quicker and stronger the reverse tolerance builds up, the less Rue/Harmalas you take, the slower and weaker the reverse tolerance builds up, so lower doses like 2.5 to 3 grams will take longer to build up the reverse tolerance as fully as say 3.5 to 4.5 grams would, especially 4 to 4.5 grams.
Now, if we're talking Psilohuasca, technically, Rue/Harmala dosage doesn't "necessarily" matter since Psilocybin/Psilocin is already orally active, however... For proper Psilohuasca, you still want to approach it in the same way as Aya/oral DMT, you want to take a good dose of Harmalas, wait 30 to 45 minutes to an hour, take the mushrooms (preferably mushroom tea), and the active gut MAO-A inhibition should properly potentiate the dosage of the mushrooms, and lengthen duration to 9 to 12 hours. But, with that said, since mushrooms are already orally active, you're more free to experiment with dosages and timing, however, the same rules apply to Psilohuasca that applies to Aya, regardless of oral bioavailability/activity of mushrooms, so you can try taking 1 to 2 grams of Rue with mushrooms, and while it might be enough to change things up a bit, it won't be like 2.5 grams to 4.5 grams of Rue, and the higher the dosage of Rue/Harmalas/Caapi, the more Aya-like it is.
But with that said, my best Psilohuasca experience to date/so far, was using 2.5 grams of Rue, 35mgs of 4-ACO-DMT, and 4 grams of Lemon Balm tea. But the time before that, i tried using 2 grams of Rue and 25mgs of 4-ACO-DMT, and yes the 4-ACO dosage was 10mgs lower which does make a difference, so too is there a difference in the 500mgs between 2 to 2.5 grams of Rue, so at the lowest, even for Psilohuasca, i'd recommend at least 2.5 grams, but ideally/preferably, 3 to 3.5 grams for a good moderate dose, and 4 to 4.5 grams for a good high dose, but if you wanna go heavier than that, i'd recommend building up the reverse tolerance and not going over 4.5 grams, more Rue isn't necessarily better, but more Harmala content certainly can be, at least once your body becomes accustomed to it and the side-effects lessen.
Also, imo, you may keep in mind that if making tea from the seed, you may not get all the goods compared to consuming the seed powder encapsulated, as while boiling some goodies may be "lost in transition", so 1 gram of boiled seed, unless being really careful to get every last drop pretty much, may not even be as potent as 1 gram of the actual seed, which further casts into doubt the potential of 1 gram being "enough", but even if you manage to get it all and it's no less potent than the actual seed, i still highly doubt 1 gram will be enough, and ime it seems to be true that 1 gram of Rue will not be enough for sufficient MAO-A inhibition. Again, maybe 2.5 grams, maybe 2 grams a little bit, but 2.5 grams really seems to be where things truly get started when it comes to Rue.
