Yopo extraction photos

[Garulfo]

Nice one. Was that from the 114g's?

Yes, 11.38 from 114 g.

I've never used an oil bath before. Is peanut oil good for that?

I don't know. I guess yes. SWIM used cooking oil (olive oil) for that purpose.

What's the best thing to put the oil in? Would bomex do the job?

I don't know what is "bomex". SWIM simply use a kitchen pan dedicated to that. Pyrex would do it but I guess that a metallic pan is better for a electric hotplate (heat conduction).

Between now and then I really want to get the process sorted in my head.

Until yet, SWIM's extraction is fairly simple. He did not even bothered to dry the mix seed+carbonate calcium before putting acetone. There was some water in the evaporating dish... and then ?
The extract is undoubtly active (bio asseyed a bit).SWIM's aim is to get the amber stuff, he is just dreaming about the crystals but do not really believe he can get them with his kitchen equipment.

 

[Garulfo]

SWIM placed 5 g of crude extract in a jar with xylene (around 50 ml). He boiled the xylene for few min and then poured it in a pyrex dish. The dish made a slope with a freezed block behind in the hope the cold would accelerate the precipitation. As you may see on that photo that simulate what SWIM did, few dark drops precipitated.
Not very encouraging...

Xylene was put in the freezer for few hours and some amber clouds were forming. SWIM thought that he could add some acetone to the xylene, then water, so that two layers would form in the idea to easily separate xylene from stuff.
But althought some dry material came, it is still very dark.

Maybe SWIM should try again but with less xylene and put it longer in the freezer.

 

[69ron]

Let the xylene sit overnight. Being in the freezer doesn't seem to help much at all. When SWIM does this with the results of a good A/B extraction (using DCM as the non-polar solvent) he always lets the xylene sit overnight. Bufotenine is insoluble in xylene, but it takes a long time for it to drop out. SWIM tried freezing the xylene and that didn't speed it up. It takes many hours whether at room temperature or freezing. Bufotenine is only soluble in boiling xylene. It’s pretty much just as insoluble in room temperature xylene as freezing xylene. In fact, freezing is not a good idea because more impurities will precipitate out with the bufotenine.

 

[kemist]

He took 114 g of yopo seeds, and mix them with 114 g of carbonate calcium and a bit of water to make a paste, placed in a jar.

sorry wasn`t calcium hydroxide suggested for basifyng wet yopo mush :?:

If so, ILPT found that with weaker seeds even 1:4 ratio (adding 1 part of calcium hydroxide to 4 parts of seeds powder) can actually overkill the pH

With sodium carbonate he couldn`t basify without adding plenty (1:1 ratio)

Is making 1:1 mix of calcium carbonate and yopo powder the way to go :?:

Isn`t it calcium carbonate weaker base then sodium carbonate?

Is that Ca2+ ion what actually making the difference?

ILPT can NOT get through this extraction with good results. He tried many different seeds and detected psychoactivity as well as side effects.

He has no problem with other extraction (DMT, harmala) because chemistry behind it is well known.
But here he KnowS FUCK ALL !(sorry for that but it`s true). He doesn`t know much about : safe pH values for bufo, theory of calcium bufotenate, other compounds in seeds, etc.

He isn`t sure whether guys talking about bufotenine or it changed to different compound and it`s no longer bufotenine but something better.

He is PISSED OFF and FRUSTRATED with this bloody cebil/vilca business

 

[soulfood]

Welcome to the club

I gave up on this a few months back, but I still have seeds sitting upstairs and I know I'll get back on it at some point.

What methods have you been using?

So far my best output has been from pulling basified material with acetone/converting to fumarte/rebased/pulling again and leaving the acetone to evap. This acheived a light brownish product that was not to much on the side effects but definately wasn't potent.

I performed a limonene boil on it to clean further and it went black after scraping it up. Damn!

Definately going to try again soon but I will use the mek:heptane cleaning phase.

 

[Phlux-]

swim washed his "pure jungle" with water - the jungle remained and the n-oxide was picked up by the water - evapped in a separate dish - yeilding a yellow oil - the red jungle is separate.
Are Bufo's solubilities simmilar enough to allow this kinda proceedure ?

 

[Garulfo]

If you wash bufo dissolved in something, it does indeed precipitate, depending on the ratio solvent/water but it's hard to tell if what precipitates is interresting or not.

SWIM performed a xylene boil as suggested by Ron (letting it sit for a while). There was two pretty distinct layers visible in the bottle. One clear above and one dark, more dense on the bottom. SWIM pulled out the clear layer and evaporated the xylene (2 days, no additional heat). The dark goo resulting was smelling very strong.
SWIM thought that some xylene was remaining (which was probably the case tough), so he washed this extrac with acetone and evaporated again.
A strong smell remain and remembered something to SWIM : it was very close to DMT smell ! many days later it does still smell the DMT.
The darker layer was washed separatly with acetone and evaporated.
It smells almost nothing (except a bit of yopo) !
The lighter extract seems giving strong unpleasant side effects once smoked (few mg).
Strangly, the darker extract seems much more pleasant, more bioassay beeing required.

SWIM guess that there is something to do with bufo extract and 'layer separation' but there is still a long road to success :?

 

[Garulfo]

Funny, I just read what Ron wrote on another bufo topic :

When he compared the lighter amber extract with the darker toasted extract, he found that the toasted extract was nearly twice as strong and with far less side effects.

Look likes very close to what I said about the two 'layers'. Note that SWIM did not toast anything.

I wonder if bufo synergize well with DMT. Maybe not, which could explain (a bit) some of the unpleasant side effects.
Toasting the seeds probably remove the DMT, like separating cloudy clear layer from the dark dense layer.

---------------------

Isn`t it calcium carbonate weaker base then sodium carbonate?

[EDIT]
Ooops I was wrong. SWIM used carbonate SODIUM, carbonate calcium is indeed too weak.

 

[Garulfo]

SWIM spoke too fast about the side effects...

Yesterday he tested again his darken extract from xylene boil. Took a deep toke from something like 5 mg (hard to weight so small piece).
The he immediatly felt a strong body load (arms, legs). What people may call vasoconstriction. He felt so heavy that he had to lay down. This lasted 10 mn. 1 mn after the toke, a sound (tinnitus) and a vibration in the head was felt (not unpleasant tough). I guess it has also to do with vasoconstriction.
When the body load was gone, the nicer effects were noticeable for around 20 mn. A second smaller toke was taken, no body load that time.
Blurry/greeny vision lasted around 20 mn. After that, some slight nausea appear.

But this morning, SWIM cough like a old smoker (althought he do not smoke at all).

So all in all, side effects+cough makes the bufo extract a not so interresting substance for SWIM. The first toke is very strong (body load) so maybe it should be done in several smaller tokes. It's somehow funny how different kind of extracts produce different kind of side effects and timings. Side effects beeing quite consistent tough from a given extract.

Note for people who feel nothing from 10 mg : what you have is not bufotenine

 

[69ron]

The results should be colorless, not dark. Something is not right. That's probably why you felt the side effects.

 

[Garulfo]

Colorless ?!!
SWIM NEVER get anything coming close to clear amber Whatever the tek used (xylene boil, limonene boil, mek:naphta)
The less colorfull stuff was dark amber and turned black anyway once scrapped.
Well, that's really a difficult tek.
Maybe doing another A/B could help...

 

[69ron]

Garulfo, look at these:

That's what bufotenine looks like under a microscope. Those photos are from SWIM. Notice the slight amber discoloring. That amber color is impurities. Bufotenine is colorless.

 

[Darkbb]

I really enjoy all these pictures people. We need more pictures at the nexus. A little eye candy thanks.

 

[Seven]

wow nice pics ron!

 

[soulfood]

Colorless ?!!
SWIM NEVER get anything coming close to clear amber Whatever the tek used (xylene boil, limonene boil, mek:naphta)
The less colorfull stuff was dark amber and turned black anyway once scrapped.
Well, that's really a difficult tek.
Maybe doing another A/B could help...

what variations of mek:naptha have you used?

Have you tried the mek:heptane exactly as stated by ron?



Has anyone tried Ron's MEK:Heptane method to the letter and not got the same results?

 

[Garulfo]

Ron, yes I already saw that photos.
That's the photos that made SWIM so eager to try a bufo extraction
But what could I say... SWIM is just jealous of thoses beautiful crystals and hardly believe he will ever reach even a crystaline amber stuff
However, SWIM has no access to DCM, nor heptane.

Have you tried the mek:heptane exactly as stated by ron?

SWIM used a 60:40 ratio MEK:naphta.
He has not heptane to play with, only a mix of hexane+heptane eventually.


Below is what SWIM could get from a xylene boil. Ron, what would be the best bet to try to improve the aspect, MEK+heptane/hexane or doing a A/B and defat (xylene ?) ?

 

[soulfood]

Have you tried the mek:heptane exactly as stated by ron?

SWIM used a 60:40 ratio MEK:naphta.
He has not heptane to play with, only a mix of hexane+heptane eventually.

Ok.. let me put on my thinking hat... not a chemistry whizz so I don't understand this fully and if someone can give me a hand... cheers

Ok so the ratio stated by Ron was 2:3 MEK:heptane... I'll say 40:60 because I like %'s better than ratios.

You're substituting heptane for naphtha, heptane is very non-polar, more so than a mix of heptane and hexane anyways right? But you are using a higher % of MEK than Ron stated for his results when using heptane. By my thinking you should be using less MEK as more impurities should be soluble in the mix you used.

I'd try 30:70 MEK:Naphtha and then play around with the ratios from there.

Does that make sense or have I missed the point?

 

[Garulfo]

Does that make sense or have I missed the point?

Hmmm thanks Soulfood, that makes sense :idea:
SWIM will try that 30 MEK:70 naphta ratio on his extracts. Maybe even 20:80 and see if the extract disolve at all, then add slowly some MEK until it disolve enough.

 

[soulfood]

Just curious.. did anyone ever try the tek noman posted in the bufo extraction thread? This part here grabbed my attention.

This is the best that my friend has tried.

"You want to start with water. If you start with alcohol, it pulls out a TON of fat and oil from the beans. If you don't mind de-fatting your extract, alcohol will do fine. De-fatting is a drag.

I'm a little intrigued here as most people seem to be starting with the ultra powerful acetone that seems to pull the whole world and his wife out of everything.

 

[Garulfo]

SWIM believe that it's easier to deal with small quantities of extracted material than with xxx g of bean+base.
That's why he prefer to get everything usefull out and work with few mg of material.
On a previous attempt, SWIM did a acid extraction (so with water first). But in the end, the extract was as dark as with a direct base+acetone and the yield was ridiculous (but that may have been the beans).