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Research 2020 Bufotenine Extraction TEK + Analytical Data

Research done by (or for) the DMT-Nexus community
Migrated topic.
An update on bioassays...

IM of 25 to 30 mg of either bufotenine fumarate or malate without MAOi gives very strong visionary effects for about 50 min with a 2 hr tail of hallucinations, OEV and CEVs. Does not seem to be close to the highest that we could go. Visions of yellow and white colored nets, sonar like pulses and overlapping crystals are common. Not as colorful as nn-dmt but just as lively. Pushing the eyelids together or rolling the eyes up changes visuals to cartoonish blue red and white Aztec motifs, as if small alterations in retina directly changes visuals. Everything else described before is intensified or maintained, especially the long duration compared to nn. Some physical discomfort occurs during the first 10 min in, like the sinking sensation and some strange muscular tightness (maybe this is the vasoconstriction) but it goes away. Nausea is consistent during the first 1 hr. I guess the body loads are the inevitable downsides and it interferes a bit (comboing with small amount of Dramamine perhaps?). At t0130 in there is a good relaxation and tranquility as the magical varnish over the world slowly disappears. Perhaps some headache at t0400-0500, but confounds with too much cigarette and end of day malaise.

Compare that with up to 50 mg oral sublingual bufotenine which gives light OEV CEVs at t0130. Clearly active but very weak absorption.

Also compare with IM at same dose of nn-dmt fumarate, giving similar peak intensities, but shorter duration, without some of the side effects.

I know there is some policy in the nexus about injections not being cool, please point me to them or let me know if I should stop reporting. 🙃
 
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Here are our Bufotenine re-X attempts-reports.

Following the CIELO-like/stb to acetone pull to fasa procedure this post has laid the grounds for, it is quite common to get a dark brown plus a light tan to white precipitate mixed together. We believe the dark stuff are pigments or lignin or whatever from seed husks while the tan stuff is magik, or at least they are different degrees of purity of the stuff we want. How do we get the dark stuff out?

Here is a suggestion for a cleanup:

To the acetone containing the precipitate (measure decanted volume), add-in water (drop-wise) and mix it hard, dissolving should be almost instantaneous then wait for all of it to decant. If only dark solids remain (from dark brown all the way to pitch black solids, a lot less than original precipitate volume) then stop adding water. Collect the liquid and add FASA to your hearts' content, clouds form and light tan precipitate form, very quickly.

Can I get a feedback on this? Kind of dangerous mixing water with magic salts.

Another not surprising observation is that completely dry bufotenine loaded acetone usually drops powder fumarates while tiny amounts of water added prior to FASA greatly increases the chance of forming crystals. This needs proper experimentation.
 
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I appreciate that Trip!

Here is my lazy attempt at imaging evidence:
buf1.jpg
Let me try to make sense of it. 1 is a dirty brown bufo that is partially dissolved in acetone + water as described before. 2 is a relatively clean bufo that is in a powdery format, pretty good but difficult to manipulate, to get it like this is a whole other tek which takes too much effort. 1a is 1 after being fully dissolved in acet+water, black stuff decanted and then the liquid was transferred over to 2, making 1in2 to which water was also added until it all dissolved (notice the orange color to the liquid). 3 is a re-Xed thing, after FASAing the orange fluid until it clouded (if just barely enough water was used, it should cloud immediately), crystals formed (magically fast) and liquid was collected and it is going in acetone washes then decants until acetone comes out clear. This is certainly not perfectly clean, but at least it doesn`t have the black gunk in it and it is extremely simple to manipulate.

So, we tested this in small scale 3 times. It works but it can be tricky. Recovery is unknown. As of now it is meant to be a post FASA re-X cleanup to get it in a state that facilitates decantation, that is, crystals not powder. A key factor seems to be maintaining the lowest volume of water needed to keep it dissolved while acetone is present, also maintain highest concentration of bufotenine possible so total volume is as minimal as possible, this should make crystals really fast.

To a brownish to dark brown material, add in minimal amount of acetone to cover it, or if you are just about done in FASA (1), decant the material within acetone and remove as much of the yellow acetone as possible. Add in water dropwise and hard mix after every drop. Brown precipitates should fully dissolve and leave very dark or dark brown solids (1a), liquid should have a strong orange color (1in2). Patiently wait for it to decant, then collect the orange liquid (orange is good! yellow means too dilute) into a new vessel with free volume. Add in FASA dropwise, it should cloud as soon as the acetone to water ratio reaches whatever level that spits salty bufotenine out of solution, eventually to get crystals like 3 or better. Stop dropping FASA when it does not cloud anymore. Decant, remove liquid and wash with acetone repeatedly until it comes out clear.

Notes: brown stuff in 1 is normal to get out of a loaded acetone. Light yellow tan stuff in 2 looks great but too much work and likely loses lots. 1a is suspiciously colored like the seeds` husks. 3 is too colored and unclean for some applications, though a great improvement over 1, but I do suspect that if it is washed enough times and then re-X using this same procedure, light yellow to almost transparents crystals can be obtained.
 
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