Alcohol extraction of Yopo

[modern]

Using 96% ethanol I soaked 100g seeds in just under 2L of ethanol. After a few weeks I finally got around to distilling the ethanol and the last 50ml was swirled and put in beaker. Tried to dissolve the goo/oil with 25ml recovered ethanol but didn’t dissolve so just poured. Added water and the goo dissolved in 100ml.

There is a blueish tone under 365nm light. I’ll try to capture later. Don’t have any TLC plates at the moment.

Planning on doing hcl to free and acids and other non alkaloid impurities. Than ammonia to Freebase it and hopefully I’ll have Freebase bufotenine once everything is evaporated? I know a more solective solvent would be a good option. Thinking a defat using frozen ethanol may be enough? Idk if it is in the oil/goo in meaningful amounts.

After some time the alcohol extracts got a little cloudy… maybe pulled some water air?

 

[modern]

Two days settling…

Water dissolved goo has a precipitate that I don’t think is bufo since ph is slightly acidic naturally or maybe partially Freebase naturally?

The alcohol has a clear separation of the oil no on top which I assume holds some bufo but most is in the alcohol?

May be a while before I finish my idea on this so just posting if anyone has any ideas or experiences

 

[modern]

So I decided to freeze the alcohol portion and got a good separation of the oil that solidified. The alcohol portion is slightly thicker like honey. The oil I decided to add to the water fraction and most “dissolved” and formed a precipitate like earlier and now much more material. Still some oil on top of the surface which I’ll later stir to see if dissolves.

I’ll attempt a TLC on the water, alcohol and the precipitate hopefully by the weekend.

The fact that the precipitate is only formed in water makes me believe it may be bufo (atleast the free bufo) since acids likely all water soluble and in alkalin solution also soluble…