Growth parameters:
B+ strain cubensis were colonized on birdseed and transferred to a bulk substrate consisting of coir, manure, straw, vermiculite, and gypsum to which 10mM of melatonin was added giving a ratio of roughly 6.3 grams of melatonin to roughly 4 kg of bulk substrate. Substrate was measured at ph 8.
TLC:
Sample = Cubensis grown on melatonin doped substrate.
PC = Cubensis grown on undoped whole brown rice.
5-meo = 5-MeO-DMT HCL standard.
Mela = Melatonin standard.
Placeholder for analysis information.
Analysis suggests the fungus created a novel, but closely related analogue of the melatonin.
Preparation:
~5 grams of whole fruits were ground to a fine powder and soaked in ~10 ml 96% EtOH for ~2 months to create a tincture.
Bioassay:
With ~1 ml (0.5 grams)
Tincture was diluted with ~2 parts water and held at the back of the throat for ~10 minutes before swallowing. Effects began around the t+20 mark. Pleasant stoning effect on par with standard low dose cubensis ramping up steadily over the course of about 30 minutes to achieve a + on the Shulgin scale. Effects then plateaud into a threshold psychedelic state with some faintly translucent, pulsing, wave-like visual effects behind the eyelids. At ~t+70 the trippy effects began to subside and the characteristic melatonin heaviness set in. The heaviness increased with quite a bit of yawning over the course of ~30 minutes then began to subside as happens when staying awake after taking melatonin. By ~t+120 mark most psychoactive effects had passed apart from a light and easily missed stoney afterglow feeling.
At ~t+150 a standard nightly dose of harmalas + melatonin (~5mg each) was administered and subject went to bed. Sleep came easily and quickly. Dreams were slightly more vivid than usual and followed an extremely cohesive and linear direction, even to the point that getting up in the middle of the night to use the restroom resulted in the dream sequence picking up right where it left off and continuing with the story through the rest of the night. Upon waking subject felt well rested and no residual hangover effects were noted.
With ~3 ml (1.5 grams)
Tincture was diluted moar heavily on the order of ~10 parts water in trying to wash ground mushroom material from filter. Tincture/tea was administered slowly over the course of ~25 minutes taking a sip, holding at the back of the throat for a few minutes, swallowing, and repeating. Effects began at ~t+20 and ramped up slowly over the course of ~30 minutes. It came on as a light stoned feeling and gradually increased over the course of ~30 moar minutes toward a + on the Shulgin scale, at which point the heavy melatonin sedation kicked in. This floaty stoned sleepy state was maintained over the course of the next hour when subject decided to go to bed around the t+120 mark. Dreams were vivid but no moarso than with standard melatonin administration. Subject awoke well rested and no hangover effects were noted.
With ~7ml (3.5 grams)
Tincture was diluted roughly 50/50 with water. Half the dose was held at the back of the mouth for exactly 5 minutes then swallowed. This was repeated with the other half. At t+10 the "dregs" of the ground of mushrooms were soaked in warm water for 5 minutes. This tea was drank over the course of about 10 more minutes. First alerts began around t+20 with unmistakable effects by t+30. The onset ramped up to a nicely stoned feeling that plateaud between t+45 and t+60. This stoned feeling remained more or less for ~60 more minutes when the standard heavy/tired melatonin effects began and became quite strong. At this point ~t+120 the subject went to sleep. Dreams were very linear and coherent as if the entire sleep cycle was spent in one stable dream scenario. Subject slept slightly longer than usual, perhaps an extra 30 minutes and woke up feeling well rested without hangover.
With ~2.5 dry grams eaten:
Pattern was similar to all previous attempts. Effects began around t+30 and built strongly in the manner typical to standard psilocybin mushrooms leveling out a strong stoned melatonin feeling. Upon sleeping dreams were very vivid, but not particularly linear. Refreshed upon waking with no feeling of hangover.
B+ strain cubensis were colonized on birdseed and transferred to a bulk substrate consisting of coir, manure, straw, vermiculite, and gypsum to which 10mM of melatonin was added giving a ratio of roughly 6.3 grams of melatonin to roughly 4 kg of bulk substrate. Substrate was measured at ph 8.
TLC:
Sample = Cubensis grown on melatonin doped substrate.
PC = Cubensis grown on undoped whole brown rice.
5-meo = 5-MeO-DMT HCL standard.
Mela = Melatonin standard.
Placeholder for analysis information.
Analysis suggests the fungus created a novel, but closely related analogue of the melatonin.
Preparation:
~5 grams of whole fruits were ground to a fine powder and soaked in ~10 ml 96% EtOH for ~2 months to create a tincture.
Bioassay:
With ~1 ml (0.5 grams)
Tincture was diluted with ~2 parts water and held at the back of the throat for ~10 minutes before swallowing. Effects began around the t+20 mark. Pleasant stoning effect on par with standard low dose cubensis ramping up steadily over the course of about 30 minutes to achieve a + on the Shulgin scale. Effects then plateaud into a threshold psychedelic state with some faintly translucent, pulsing, wave-like visual effects behind the eyelids. At ~t+70 the trippy effects began to subside and the characteristic melatonin heaviness set in. The heaviness increased with quite a bit of yawning over the course of ~30 minutes then began to subside as happens when staying awake after taking melatonin. By ~t+120 mark most psychoactive effects had passed apart from a light and easily missed stoney afterglow feeling.
At ~t+150 a standard nightly dose of harmalas + melatonin (~5mg each) was administered and subject went to bed. Sleep came easily and quickly. Dreams were slightly more vivid than usual and followed an extremely cohesive and linear direction, even to the point that getting up in the middle of the night to use the restroom resulted in the dream sequence picking up right where it left off and continuing with the story through the rest of the night. Upon waking subject felt well rested and no residual hangover effects were noted.
With ~3 ml (1.5 grams)
Tincture was diluted moar heavily on the order of ~10 parts water in trying to wash ground mushroom material from filter. Tincture/tea was administered slowly over the course of ~25 minutes taking a sip, holding at the back of the throat for a few minutes, swallowing, and repeating. Effects began at ~t+20 and ramped up slowly over the course of ~30 minutes. It came on as a light stoned feeling and gradually increased over the course of ~30 moar minutes toward a + on the Shulgin scale, at which point the heavy melatonin sedation kicked in. This floaty stoned sleepy state was maintained over the course of the next hour when subject decided to go to bed around the t+120 mark. Dreams were vivid but no moarso than with standard melatonin administration. Subject awoke well rested and no hangover effects were noted.
With ~7ml (3.5 grams)
Tincture was diluted roughly 50/50 with water. Half the dose was held at the back of the mouth for exactly 5 minutes then swallowed. This was repeated with the other half. At t+10 the "dregs" of the ground of mushrooms were soaked in warm water for 5 minutes. This tea was drank over the course of about 10 more minutes. First alerts began around t+20 with unmistakable effects by t+30. The onset ramped up to a nicely stoned feeling that plateaud between t+45 and t+60. This stoned feeling remained more or less for ~60 more minutes when the standard heavy/tired melatonin effects began and became quite strong. At this point ~t+120 the subject went to sleep. Dreams were very linear and coherent as if the entire sleep cycle was spent in one stable dream scenario. Subject slept slightly longer than usual, perhaps an extra 30 minutes and woke up feeling well rested without hangover.
With ~2.5 dry grams eaten:
Pattern was similar to all previous attempts. Effects began around t+30 and built strongly in the manner typical to standard psilocybin mushrooms leveling out a strong stoned melatonin feeling. Upon sleeping dreams were very vivid, but not particularly linear. Refreshed upon waking with no feeling of hangover.
