Ok I've got a little free time coming up and I am once again going to face my nemisis that is the bufotenine extraction. As I have little oppotunity for this kind of thing these days I really want to get it right this time. I've done lots of reading here and there trying to figure out what's gone wrong in previous attempts and generally finding out better ways of working this molecule. I'm pretty sure there's nothing on this site about bufotenine I haven't read at least once.
This is going to be the procedure I am planning to use this time round with comments and questions here and there for the sections I am not sure about.
1. Toast seeds at 500f until the popping stops.
2. Grind up seeds to a fine powder
3. Run acetone through seeds until acetone comes out clear.
Ron reports his SWIM having good results when using DCM for his defats. What is the difference if any when using DCM instead of acetone? I can't acquire DCM, but I have also read of people using room temperature xylene for this part of the process. I always figured acetone to be a much stronger solvent than xylene. Basically other than reading about relevant alkaloids solubility with the common alkaloids known to this forum I have zero knowledge on solvents.
4. Basify material with sodium carbonate/air dry.
5. Rinse material thoroughly with acetone.
6. Acidify acetone(FASA/CASA). Collect precipitates.
This is my main point of interest here really. I always used fumaric acid at this point, because I figured the resulting material would be much easier to handle. However I recall reading somewhere that it is better to use citric acid for bufotenine, but there weren't any details on why. If I rinsed either end product with acetone could I expect different levels of purity in either product?
I understand the two substances have a very similar level of acidity, but could there be some other property that makes the two substances different?
Also what are the advantages of evapping the acetone to a smaller volume before acidifying, besides a more manageable quantity?
7. Basify extract with sodium carbonate.
Would it be a good idea to do this process in solution rather than the dry/paste method? I know I don't have access to a suitable solvent for extracting bufotenine from water, but I'm interested if there are any toxins that would be more water soluble than bufotenine if I just shoot the ph high enough to basify the bufotenine?
8. Dry/heat in the oven.
This is the first heating that I will be using in the base state. I know most people toast the seeds again like they were preparing a cebil snuff before they start the rest of the extraction, but are there any disadvantages in waiting until this stage to toast the extract?
9. Pull with acetone/evap
10.... Well hopefully by this stage I will be looking at something worth purifying and will try anything but a limonene boil.
Only other thing is, has anyone acheived 95% pure + bufotenine when not using argentinian seeds?
So hopefully all you beautiful, kind and smart people
will let me know what you think. I know this sort of thing has been asked many times before so thanks to those who made it this far. I'll probably be starting this up in just under a week so I'll post my updates here.
This time here's hoping.
This is going to be the procedure I am planning to use this time round with comments and questions here and there for the sections I am not sure about.
1. Toast seeds at 500f until the popping stops.
2. Grind up seeds to a fine powder
3. Run acetone through seeds until acetone comes out clear.
Ron reports his SWIM having good results when using DCM for his defats. What is the difference if any when using DCM instead of acetone? I can't acquire DCM, but I have also read of people using room temperature xylene for this part of the process. I always figured acetone to be a much stronger solvent than xylene. Basically other than reading about relevant alkaloids solubility with the common alkaloids known to this forum I have zero knowledge on solvents.
4. Basify material with sodium carbonate/air dry.
5. Rinse material thoroughly with acetone.
6. Acidify acetone(FASA/CASA). Collect precipitates.
This is my main point of interest here really. I always used fumaric acid at this point, because I figured the resulting material would be much easier to handle. However I recall reading somewhere that it is better to use citric acid for bufotenine, but there weren't any details on why. If I rinsed either end product with acetone could I expect different levels of purity in either product?
I understand the two substances have a very similar level of acidity, but could there be some other property that makes the two substances different?
Also what are the advantages of evapping the acetone to a smaller volume before acidifying, besides a more manageable quantity?
7. Basify extract with sodium carbonate.
Would it be a good idea to do this process in solution rather than the dry/paste method? I know I don't have access to a suitable solvent for extracting bufotenine from water, but I'm interested if there are any toxins that would be more water soluble than bufotenine if I just shoot the ph high enough to basify the bufotenine?
8. Dry/heat in the oven.
This is the first heating that I will be using in the base state. I know most people toast the seeds again like they were preparing a cebil snuff before they start the rest of the extraction, but are there any disadvantages in waiting until this stage to toast the extract?
9. Pull with acetone/evap
10.... Well hopefully by this stage I will be looking at something worth purifying and will try anything but a limonene boil.
Only other thing is, has anyone acheived 95% pure + bufotenine when not using argentinian seeds?
So hopefully all you beautiful, kind and smart people
will let me know what you think. I know this sort of thing has been asked many times before so thanks to those who made it this far. I'll probably be starting this up in just under a week so I'll post my updates here.This time
here's hoping.
