• Members of the previous forum can retrieve their temporary password here, (login and check your PM).

Epifluorescence microscopy of Rue alks (PICS!)

Migrated topic.

deedle-doo

Rising Star
I was curious about the fluorescence of Rue alkaloids so I performed a quick epifluorescence analysis. Basically, I have a microscope that can direct certain wavelengths of light at a sample and detect different wavelengths of light. This allowed me to check out the fluorescence of some Rue alkaloids I'd preped a couple of years ago. The results were a little surprising.

Rue alks have a broad spectrum of fluorescence. I knew about the blue->green fluorescence but I also found UV->blue fluorescence and green->red fluorescence. The blue->green fluorescence was about 2x more intense then the others though.

Check it out:
 

Attachments

  • rue.jpg
    rue.jpg
    177.5 KB · Views: 0
  • rue+10x.jpg
    rue+10x.jpg
    902.6 KB · Views: 0
  • rue+63x.jpg
    rue+63x.jpg
    439.3 KB · Views: 0
  • nacl+control.jpg
    nacl+control.jpg
    158.5 KB · Views: 0
Back
Top Bottom