Grafted MHRB cultures

[1ce]

I'm toying with the idea of micropropagation/hair root cultures and callus cultures for mimosa tenuiflora. Specifically what I have in mind is first trying a callus culture and extracting from it directly, using a mimosa sapling as feedstock.

Second I propose organogenesis of the roots, and grafting them into an existing non mimosa/mimosa plants, then innoculating with hairy root disease to grow these plants along much quicker.

Hairy root culture for mass-production of high-value secondary metabolites - PubMed

Plant cell cultivations are being considered as an alternative to agricultural processes for producing valuable phytochemicals. Since many of these products (secondary metabolites) are obtained by direct extraction from plants grown in natural habitat, several factors can alter their yield. The...

www.ncbi.nlm.nih.gov

https://www.researchgate.net/publication/226018305_Studies_onMimosa_tenuiflora_callus_culture_Interaction_of_kinetin_and_24_dichlorophenoxyacetic_acid_in_initiation_and_growth

Also, has anyone tried grafting mimosa, or growing hydroponically?

I've also had minor success with the micropropagation of phalaris aq1 strain starting with protoplast isolation. I'd also like to try fusing protoplasts of aq1 with endogenous phalaris grasses and profiling their alkaloid content. I'm looking to bounce ideas around and discuss methods and possible experiments.

 

[kasvi]

I know this is old thread but have you seen this?

https://www.researchgate.net/publication/8031420_Variation_in_the_accumulation_levels_of_NN-dimethyltryptamine_in_micropropagated_trees_and_in_in_vitro_cultures_of_Mimosa_tenuiflora

"In all cases, the neurotoxic compound N,N-dimethyltryptamine (DMT) was detected with the lowest concentration 0.01% dry weight (DW) in flowers, and the highest 0.33% DW in bark. For the in vitro cultures, DMT was present in high yields in plantlets (0.1-0.2% DW), while in calluses this compound was initially detected but its concentration decreased significantly in the subsequent subcultures."

Can you share those papers that you linked if you have access to them?
I would like to read these too:

https://www.researchgate.net/publication/226018305_Studies_onMimosa_tenuiflora_callus_culture_Interaction_of_kinetin_and_24_dichlorophenoxyacetic_acid_in_initiation_and_growth

Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview - Trees

Trees are an integral part of human life, and a vital component of biodiversity. Forest trees in particular are renewable sources of food, fodder, fuel wood, timber and other valuable non-timber products. Due to the rapid growth of population and the human desire to progress, there has been a...

link.springer.com

In vitro propagation ofMimosa tenuiflora (Willd.) Poiret, a Mexican medicinal tree - Plant Cell Reports

Mimosa tenuiflora (Willd.) Poiret (Leguminosae) was micropropagated throughin vitro culture of axillary buds on Murashige and Skoog (MS) medium. Shoot formation was achieved when the media were supplemented with 0.1 mg.L−1 IAA + 3 mg.L−1 KN.In vitro rooting of regenerated shoots was achieved...

link.springer.com

Large-scale production of hairy root - PubMed

Many products of interest are synthesized in organized tissues, but not formed in suspension or callus culture. Therefore, most attention has been focused on root cultures. The transgenic plant,"hairy root", has brought us to dramatic improvements in growth rate and high content of desirable...

www.ncbi.nlm.nih.gov

I have 5 Mimosa hostillis seeds that I think I will germinate this spring. I can grow them hydroponicaly to see if I can grow lot of roots that way. What hydroponic system you would recommend I use?

 

[dreamer042]

Srivastava, Smita, and Ashok K. Srivastava. "Hairy root culture for mass-production of high-value secondary metabolites." Critical Reviews in Biotechnology 27.1 (2007): 29-43.

Nicasio, Mdel P., et al. "Variation in the accumulation levels of N, N-dimethyltryptamine in micropropagated trees and in in vitro cultures of Mimosa tenuiflora." Natural product research 19.1 (2005): 61-67.

Giri, C. C., B. Shyamkumar, and C. Anjaneyulu. "Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview." Trees 18.2 (2004): 115-135.

Villarreal, Ma Luisa, and Gabriela Rojas. "In vitro propagation of Mimosa tenuiflora (Willd.) Poiret, a Mexican medicinal tree." Plant cell reports 16.1-2 (1996): 80-82.

Uozumi, Nobuyuki. "Large-scale production of hairy root." Recent Progress of Biochemical and Biomedical Engineering in Japan II. Springer Berlin Heidelberg, 2004. 75-103.

 

[dreamer042]

oops missed one.

Villarreal, Ma Luisa, et al. "Studies on Mimosa tenuiflora callus culture. Interaction of kinetin and 2, 4 dichlorophenoxyacetic acid in initiation and growth." Biotechnology letters 15.7 (1993): 721-726.

 

[kasvi]

Thanks a million times! This helps a lot.