I finally got a mass spec

[benzyme]

all sorts of samples.
going to do it legit, going to get the instrument validated.

and you're right, 5K is a great deal. the roughing pump alone goes for $1700+ used.

 

[Ulim]

So nice to have well equipped members around.

 

[benzyme]

there she is, the LC-MS

 

[Praxis.]

Now that is one damn sexy piece of machinery if I ever saw one.

This is an indispensable resource to the nexus community, thank you 8)

 

[benzyme]

and thank you.
I am grateful to be a part of this community.

 

[benzyme]

the standard.

 

[benzyme]

 

[benzyme]

8)

 

[dyed-danny]

You're able to power that entirely via solar panel power?
That's awesome! Talk about some serious home science.

 

[benzyme]

yes,thanks.
while the ion source is off, it draws about 1 kW. with it on, it would probably be around 3 kW.

I'm generating 3-4 kW during peak hours, 11a-2p.

 

[Orbiting]

very cool; by what method are you getting your ionization ?

 

[benzyme]

electrospray.

I may invest in a nanospray emitter eventually, then a newer machine (LTQ)

 

[○]

So exciting. Benz you da man. 8)

 

[benzyme]

status update:
I just got the nitrogen and UHP helium. I ordered calibration solution, and prepared a 10 pg/nL standard solution of tryptamine hcl in 50:50 isopropanol:water.
I'll baseline the instrument this weekend, and hopefully do a few test runs on the standard.

 

[hortwizard]

Will you be able to test out Caapi Specimens as well? I have some interesting vine samples in the plant vault that definately have a unique character to them.

 

[benzyme]

yes.
you could test just about anything with lc-ms.

 

[benzyme]

I connected the UHP helium and nitrogen.
for the first time since I got the instrument, I turned on the detector.


I'll baseline the next couple days, calibrate, and run tryptamine. stay tuned. (n.p.i.)

 

[Jees]

...stay tuned.

Yes!
I don't understand those screen shots but it's exiting nonetheless

 

[benzyme]

the screenshots tell me that there is residual calibrator solution in the instrument, probably the heated ion capillary (the inlet leading to the analyzer), and that it is out of tune (those numbers vary slightly from the actual values).

this is why I need to "baseline", to get a low noise signal, then optimize the analyzer voltages, and recalibrate, then tune

 

[downwardsfromzero]

Exciting stuff indeed.

On those screen shots the scale only goes down to 100 m/e and seems decidedly coarse. Presumably when it's up and running you'll be able to look in finer detail at smaller stuff? Most of what we're interested in will be weighing in at well below 500 amu.

Do the outputs pictured represent output from the detector? If so, what are those heavy peaks it's seeing?

Edit: I see you just covered some of these questions while I was typing