I finally got a mass spec

[benzyme]

normal scan range is 15-2000 amu..
since it's ESI, it can fragment 100,000 amu macromolecules.

I'll get into zoom scan mode later, when looking for specific analytes, and ms/ms when elucidating isomers.

 

[downwardsfromzero]

That's great, you really have got yourself a bargain there!

 

[benzyme]

oh believe me, I know.

thanks.

I'm bidding on a Thermo Surveyor MS pump/autosampler/PDA detector stack. If I can get it for 1500 or less, that's another steal.
I already have all the software, cables, and even configurations on the comp. the whole shebang would sell for 25K on ebay, I'd have it for less than 7500.

 

[benzyme]

gettin there.


still baselining.
notice, the range is set to tryptamine, phenethylamine, ergoline, and beta-carboline range.

NL will be around E^ 7 when optimized.

been troubleshooting all day to get all diagnostics to pass, namely the RF modulation and frequency, and optics lens voltage offset. it is now running like a swiss watch. 8)

just need to calibrate and tune. waiting on calibration solution

 

[1ce]

Well this is very exciting

I've been away far too long! Benzyme this is awesome!

 

[benzyme]

thank you.

I was formally trained in the art of LC-MS/CE-MS, and am at a decent point financially, so I pulled the trigger on a hell of a deal. Just ordered an HPLC module too, same one I used in school. I'll get this all working like a well tuned system, with sensitivity in the femtomole regime, shortly.

 

[benzyme]

 

[Lichen]

Nexus historical moment right here - proud of you Benz.

 

[benzyme]

thank you.

it's...challenging, rewarding, and always a learning experience. I learn something everytime I power it up.
yesterday, I noticed the electrospray plume under low lighting, it was a faintly glowing purple color.

[YOUTUBE]

and I put in an order for reserpine standard. with that, I can properly tune this instrument as well as a number of other instruments, and perform a validation. I already have calibration solution, as is depicted in the video.

 

[Jees]

Exiting,
make sure you keep Beaker in check

 

[Ulim]

BEFORE ITS TOO LATE

Fine equipment though. I still cant decide which direction in chemistry to go :?

Its hard to find something that I really want.

Will expand to make a huge analytical lab? With even more equipment?

Thats actually not that bad. If you get a good lab going you can have plenty of work and thus even more equipment.

 

[benzyme]

If I do, it would be an FTIR spec. NMR isn't practical in a house.. but as you can see, mass spec is. :d
maybe I'll lease a space eventually, but I really just want to master mass spec, and do some more method development. perhaps
create some inventions along the way.

 

[benzyme]

I'm still waiting on Alfa to review my account, and send me the reserpine standard. Once I get that, and properly tune, I will report back and begin accepting samples. I look forward to elucidating the efficacy of certain procedures regarding extraction of cubes and the like. I have analyzed that before.

 

[benzyme]

Reserpine arrives tomorrow.

meanwhile, I was able to run an old sample this weekend...

 

[Jees]

Reserpine arrives tomorrow.

meanwhile, I was able to run an old sample this weekend...

This sample was not reserpine then, since it's mollar mass is 608.68 and that m/z peak is nowhere any integer number of z

Do I make sense somehow or display stupidity here?

 

[benzyme]

you are correct.
the sample is a smaller molecule, and much more... mind-manifesting.

 

[Poekus]

Nice gear! Does this measure also the quantity of a molecule in a sample or only if it's present?

 

[benzyme]

both.

TIC (total ion count) chromatograms can give quantitative information about the relative amounts
in a sample. MS/MS data gives structural information.

 

[downwardsfromzero]

the sample is a smaller molecule, and much more... mind-manifesting.

That's quite a large peak around m/z 188. Hmmm....

 

[benzyme]

for those unsure of what Reserpine is for...
basically, the ion trap mass spectrometer makes measurements based on resonant frequency, a fixed freq., and is tuned to have highest sensitivity to 609.2 m/z. Reserpine is the tuning standard. after an acceptable level of sensitivity (aka NL, normalized level) is attained, a calibration is performed.