please correct me in any way shape or form, i basically have been reading several sites so i am going to jot down what i am about to do, i have copied all my ideas and i will list sources 
dreamoar042 and oly thank you for your help in chat!
first thing i am going to do is liquid culture? why you ask well, dreamoar042 said it will inoculate faster since it has already colonized and he said my syringes will go farther. which i cant complain. thank you again
# Light malt extract (or extra light. The lighter the better, because it will make it easier to observe growth in the jar.)
# Dextrose (glucose)
this is what im not to sure on! ok im thinking 1 tablespoon dextrose and 1 tablespoon light malt extract per 500ml (2 cup) water.
hot or warm water before adding sugars to allow for quicker dissolving. place a marble in the jar for agitation. Wrap top with aluminum foil and place jar in pressure cooker and slowly bring it up to 15 psi. for 15-20 min.
place lid on rubber side down, with lid and have an inoculation and extraction hole. inoculate the jar 1 cc per 2 cups of liquid culture.... yumm!
Once inoculated stick in a DARK place with a temp of 82-86F.Signs of growth after one week max, and fully done at week 3 max. Some see growth in under a day and fully done in 3. Once growth has slowed down (done), either use immediately or store in a fridge.
NEXT!!!! how exciting ^^ now that, that is down its time to knock up my patty cakes!
Reynolds-brand oven bags small is 10" by 16", rated to hold 4 pounds weight and oven-safe at up to 400° F.
They make dandy 'jars' for PF-style substrate in bulk amounts. Just mix up enough rice/verm. for X # of jars, (see note at bottom for a few ideas on this part) spoon into the bag, tie the bag closed, bake at 250° F for 45 min. Allow to cool in the oven until safe to touch. Stick the now cooled oven bag inside another, larger bag, ziplock and let cool to room temp. When cool, one can inject liquid culture right through the plastic (seal hole with tape). Once white growth becomes visible in a few days, speed up colonization by shaking the bag, and mixing the patches into uncolonized areas.
* Double bag the substrate - i.e. fill a regular size oven bag with the substrate, seal that by twisting and the tie included in the box (heat-resistant, non-metallic for microwave fans), then place that inside yet another, larger oven bag and seal it likewise.
* Squeeze most of the air out of the inner bag so steam has room to expand and leave some air in the outer large bag, so as the inner bag cools, any air sucked in is also sterile.
* Once "baked" for 45 minutes, pop the entire thing in yet a larger third ziplock storage bag, the kind that can handle hot foods. Then into the fridge to cool.
* Use an electric mixer on low to mix up: 3 cups Vermiculite, 1 1/2 cups brown rice flour, 1 1/2 cups water,in a large bowl per bag!
* When filling the oven bags, fold back the rim several inches, so no foodstuff gets on the bag near the neck/seal.
* When inoculating, don't let the needle touch the substrate, just raise the plastic above the 'cake' an inch or so & squirt spores everywhere, about 3 cc will do plenty.
* After inoculation, throw several into a black plastic trash-bag 75-85 degrees F for several weeks keep out the light & help prevent contams.
ok so i have made my liquid culture and now i have my patty cakes done ready for fruiting, mwhahahahah!
since it will have been like a month that has past im going to start steps one and two over again so that when my fruits have been harvest the next batch will be ready by then:idea:
im getting a cake dish, or foil what ever is laying around and i will cut the cake out of the zip lock Reynolds and have the cake lay naked with the frosting shinning! 90-95 humidity; 75-80 degrees F; is a good range for fruiting. i will put them in my fruiting chamber! ( http://www.caligrowkits.com/product2.php ) get my perlite nice and damp and not soupy. and i will set the timer for the grow light on a 12/12.
pick and dry... that cant be too hard. the hardest part is choosing which 81 strains of Cubensis i wont to grow, i believe house said penis envy.... any feed back would be great thanks a million!
www.shroomery.org
http://www.shroomery.org/8677/Hips-Bulk-Tek
www.shroomery.org
dreamoar042 and oly thank you for your help in chat!first thing i am going to do is liquid culture? why you ask well, dreamoar042 said it will inoculate faster since it has already colonized and he said my syringes will go farther. which i cant complain. thank you again
# Light malt extract (or extra light. The lighter the better, because it will make it easier to observe growth in the jar.)
# Dextrose (glucose)
this is what im not to sure on! ok im thinking 1 tablespoon dextrose and 1 tablespoon light malt extract per 500ml (2 cup) water.
hot or warm water before adding sugars to allow for quicker dissolving. place a marble in the jar for agitation. Wrap top with aluminum foil and place jar in pressure cooker and slowly bring it up to 15 psi. for 15-20 min.
place lid on rubber side down, with lid and have an inoculation and extraction hole. inoculate the jar 1 cc per 2 cups of liquid culture.... yumm!
Once inoculated stick in a DARK place with a temp of 82-86F.Signs of growth after one week max, and fully done at week 3 max. Some see growth in under a day and fully done in 3. Once growth has slowed down (done), either use immediately or store in a fridge.
NEXT!!!! how exciting ^^ now that, that is down its time to knock up my patty cakes!
Reynolds-brand oven bags small is 10" by 16", rated to hold 4 pounds weight and oven-safe at up to 400° F.
They make dandy 'jars' for PF-style substrate in bulk amounts. Just mix up enough rice/verm. for X # of jars, (see note at bottom for a few ideas on this part) spoon into the bag, tie the bag closed, bake at 250° F for 45 min. Allow to cool in the oven until safe to touch. Stick the now cooled oven bag inside another, larger bag, ziplock and let cool to room temp. When cool, one can inject liquid culture right through the plastic (seal hole with tape). Once white growth becomes visible in a few days, speed up colonization by shaking the bag, and mixing the patches into uncolonized areas.
* Double bag the substrate - i.e. fill a regular size oven bag with the substrate, seal that by twisting and the tie included in the box (heat-resistant, non-metallic for microwave fans), then place that inside yet another, larger oven bag and seal it likewise.
* Squeeze most of the air out of the inner bag so steam has room to expand and leave some air in the outer large bag, so as the inner bag cools, any air sucked in is also sterile.
* Once "baked" for 45 minutes, pop the entire thing in yet a larger third ziplock storage bag, the kind that can handle hot foods. Then into the fridge to cool.
* Use an electric mixer on low to mix up: 3 cups Vermiculite, 1 1/2 cups brown rice flour, 1 1/2 cups water,in a large bowl per bag!
* When filling the oven bags, fold back the rim several inches, so no foodstuff gets on the bag near the neck/seal.
* When inoculating, don't let the needle touch the substrate, just raise the plastic above the 'cake' an inch or so & squirt spores everywhere, about 3 cc will do plenty.
* After inoculation, throw several into a black plastic trash-bag 75-85 degrees F for several weeks keep out the light & help prevent contams.
ok so i have made my liquid culture and now i have my patty cakes done ready for fruiting, mwhahahahah!
since it will have been like a month that has past im going to start steps one and two over again so that when my fruits have been harvest the next batch will be ready by then:idea:
im getting a cake dish, or foil what ever is laying around and i will cut the cake out of the zip lock Reynolds and have the cake lay naked with the frosting shinning! 90-95 humidity; 75-80 degrees F; is a good range for fruiting. i will put them in my fruiting chamber!
( http://www.caligrowkits.com/product2.php ) get my perlite nice and damp and not soupy. and i will set the timer for the grow light on a 12/12. pick and dry... that cant be too hard. the hardest part is choosing which 81 strains of Cubensis i wont to grow, i believe house said penis envy.... any feed back would be great thanks a million!
Shroomery - Liquid Culture Basics
Liquid tissue cultures are used to expand mycelium into a liquid solution to inoculate your chosen substrate.
www.shroomery.org
Shroomery - Simple Cubensis Growing Technique
An outstanding guide, liberally illustrated with color photographs. It details many things which aren't in the older teks.
www.shroomery.org
