• Members of the previous forum can retrieve their temporary password here, (login and check your PM).

Proper Cell Lysis?

Migrated topic.
Toshido

Toshido

Research & Development
I've been patiently gathering extraction data by changing only one variable at a time using this tek... Widderic's Tek

Here are my findings so far.

DEx03QK.png


Does this look normal in terms of yield changes?

According to Cybs Max Ion

Cyb said:
Freeze Thaw

Cover your plant material with filtered water.
{Preferably powdered but with as much surface area
as possible}

The ionic strength is then certain to be higher inside
the cells and they will absorb a lot more of the water
by diffusion. Filtered water has a higher ionic
strength than other types.

Heat this mixture lightly for about an hour
This is to soften the cell walls and allow for
maximum water build up inside the cell walls.

This is then to be frozen and thawed several
times.(x3)

This will cause the already swollen cells to burst and
free the alkaloids from within them.
Click to expand...

From my findings, you can see I am using 250mL water and 50g powdered MHRB. I place that in a 1000mL beaker, mix well, and cover with cling wrap before introducing to a freezer at -17 degrees celsius (lowest my freezer goes) for 24 hours. I then take it out to thaw on it's own over the course of 6 hours before beginning my extraction, where I then add the beaker to a hotplate and heat to 70C, before adding 50mL vinegar. It stays at 70C for 45min before introducing the saline solution, heating for another 15 minutes before straining and basifying.

Only difference is I'm using distilled water instead of filtered, and I'm not preheating before freezing and thawing.

(Cybs says to use filtered water and to heat your soup before freezing it)

I should just get a Sonicator.
 
10× freeze/thaw sounds like a PITA :lol: How confident are you about the accuracy of your weighing? You'd want to do several repeats of the suspected higher-yielding OCD freeze thaw cycling just to make sure it wasn't, say, a random hot spot in the bark sample.
 
downwardsfromzero said:
10× freeze/thaw sounds like a PITA :lol: How confident are you about the accuracy of your weighing? You'd want to do several repeats of the suspected higher-yielding OCD freeze thaw cycling just to make sure it wasn't, say, a random hot spot in the bark sample.
Click to expand...

I measure exactly 50g powdered MHRB from the same 1000g containing bag. SO it should be mixed as thoroughly as it possibly can. I have a kitchen scale, a gram scale, and milligram scale so I haven't deviated from any of my suspected weights.

But yeah 10x freeze thaw lol it's too much!

Going to repeat again today as I have freeze thawed a bunch. I will keep you in the loop.
 
Back
Top Bottom