Protocol for DMT extraction using a Lab/scientific Reagents

[NeuroExplorer]

Hi All,

Just wondering if anyone has a protocol or tek they can recommend that uses high quality scientific grade reagents?

I see a lot of protocols that use over the counter solvents, and believe there must be a cleaner method.
Any recommendations are appreciated.

Cheers

 

[DreaMTripper]

Heptane is the most DMT selective, chloroform and DCM for plant alkaloid research.

 

[Infundibulum]

Hi All,

Just wondering if anyone has a protocol or tek they can recommend that uses high quality scientific grade reagents?

I see a lot of protocols that use over the counter solvents, and believe there must be a cleaner method.
Any recommendations are appreciated.

Cheers

There isn't such a protocol - but of course you need to explain us what do you mean by a "cleaner method"

Any method is only as clear or as dirty as the way you perform it. There is really no need for lab grade reagents, unless you example us a said need. Procuring lab grade reagents is also more risky as you may draw unwanted attention.

 

[NeuroExplorer]

Heptane is the most DMT selective, chloroform and DCM for plant alkaloid research.

Thanks!

What are your thoughts on Diethyl ether?

 

[NeuroExplorer]

There isn't such a protocol - but of course you need to explain us what do you mean by a "cleaner method"

Any method is only as clear or as dirty as the way you perform it. There is really no need for lab grade reagents, unless you example us a said need. Procuring lab grade reagents is also more risky as you may draw unwanted attention.

[/quote]

Indeed, more explanation is required.

From what i have read, Naptha seems to be the most common solvent used. However, i assume this is due to its accessibility. I was wondering what would be the best reagents to use, given no restraints due to accessibility?

Also, if i had access to equipment (such as centrifuges) could i simplify my method in any way? Perhaps i could remove lipid content by centrifuging the solution and aliquotting the supernatant?

Thanks for the help.

 

[alert]

Naphtha and heptane are used because they are fairly selective for DMT and allow you to freeze precipitate out the product from the solvent. You aren't going to get better or more pure DMT using another solvent.

Also, if i had access to equipment (such as centrifuges) could i simplify my method in any way? Perhaps i could remove lipid content by centrifuging the solution and aliquotting the supernatant?

Simplify your method? Seems to me you are trying to make it overly complex...

 

[benzyme]

Heptane and naphtha are not selective for DMT.
There's no such thing as a selective solvent.

 

[Parshvik Chintan]

just use vegetable oil :d

 

[alert]

Heptane and naphtha are not selective for DMT.
There's no such thing as a selective solvent.

selective solvent [si′lek·tiv ′säl·vənt]
(chemical engineering)

A solvent that, at certain temperatures and ratios with other materials, preferentially dissolves more of one component of a liquid or solids mixture than of another, thereby permitting partial separation.

 

[benzyme]

we use partition coefficients to determine which solvents are ideal for dissolving a solute, and which are more suited to recrystallizing; but a single solvent alone isn't specific to any solute. that's what solvent partitioning and/or chromatography are for.

 

[NeuroExplorer]

Heptane and naphtha are not selective for DMT.
There's no such thing as a selective solvent.

Do you have any recommendations for me benzyme, considering my background is not in analytical chemistry (but molecular biology) and thus I am not too familiar with techniques such as liquid chromatography?

 

[benzyme]

tech grade methylene choride and rubber cement thinner (heptane).
the former you don't even need to heat, just good ventilation
for it to evaporate. then redissolve the resulting goo in heated heptane (heat up a container/pot of water, to put
the glass container with, say, 20-30 mL heptane in). freeze the heptane overnight.
that's freeze precipitation, a method of recrystallization.

 

[c13]

Would ether be a good use in extraction or for recrystalization?

I'm just starting to understand partition coefficients
but I also thought that some solvents were " selective" in what they dissolved?
Cause I defat with three or four diff solvents because it appears that each one used grabs
some gunk that others do not. ( turpentine, xylene, naph )
Turpentine removes so much crap. Especially w/ Acacia
At least this is my theory...

 

[endlessness]

If you have access to a propper lab (and aren`t just sneaking into your university`s lab to extract scheduled substances while the lab chief is out for a coffee break ) , I think there are indeed a few things that can make your life easier.

Sonicator to help during the acqueous extraction of plant material

Magnetic stirrer when making pulls

Separatory funnel for separating non-polar pulls and/or when washing the non-polar with dilute sodium carbonate solution

Rotavap for evaporating solvent and retrieving alkaloids (this one I never used myself but I`m sure it`s awesome)




Oh and c13, dont defat with different solvents, rather, defat with the same solvent you will do your pulls with, otherwise it`s very wasteful. Actually I`ll go right ahead and say that defat is anyways a very wasteful and unnecessary process. We have come a long way since the defat days.

 

[Du57mi73]

Would ether be a good use in extraction or for recrystalization?

I'm just starting to understand partition coefficients
but I also thought that some solvents were " selective" in what they dissolved?
Cause I defat with three or four diff solvents because it appears that each one used grabs
some gunk that others do not. ( turpentine, xylene, naph )
Turpentine removes so much crap. Especially w/ Acacia
At least this is my theory...

I find this intriguing. I've thought about testing this but I haven't found it necesarry since I only use mhrb.

 

[c13]

Heptane is the most DMT selective, chloroform and DCM for plant alkaloid research.

any thoughts on use of ether