SWIM thinks the sealed container might be necessary, as he has had unsuccessful attempts with an open container. MIght be another factor though...
Good call regarding the pressure, plaincoil. Large IPA or peroxide bottles seem to work well for holding that pressure. Hopefully the bio-assays turn out successful.
Clever choice Plaincoil and astute observation Amor fati!
I wonder what amount of water or water droplets can remain in the limonene ,actually turning co2 into carbonic acid. However it is ,even the atmosphere of the container has some water vapor so it cannot be considered anhydrous which could actually help the proccess.
How exactly? Well i think a piece of the puzzle comes from another "sibling" topic, that of HCl gassing ,where HCl gas was bubbled through the non polar solvent to yield the total alkaloidal content as salt precipitate. Bear with me for a bit:
In "The nook" ,there was a thread by a member called "Me!" (who apparently seems to be missing now) on HCl gassing. He commented that while gassing with a submerged hose the crystals that formed where too small ,sometimes passing through filters : keep in mind though that he did not elaborate what filters he used so i assume coffee filters so i wonder if things are different with vacuum filtration. So ,and here comes the nice part, he tried passive diffusion of Hcl :He did not
bubble through his non polar layer, but rather
pressurised slightly the vessel with HCl gas.The result? Slowly the gass diffused through the non polar , giving BIGGER crystal formations! It worked and actually gave better results!
For those with access over at nook here are the threads (i hope linking to other forums is not agains Nexus policy,please inform me if it is so!):
So, one could try a sealed vessel, that is increased pressure. And this also brings in the topic the use of a small quantity of dry ice : Granded ,its not as easily found as seltzer water but still, its worth a try. If seltzer works i cant see why dry ice in water wouldnt work. Also an experiment would be interesting to see if lower temperatures could help the procedure: They could both lower
mescaline solubility in the non polar layer, plus increase solubility of CO2 in water/non-polar (gases generally follow the opposite principle compared to solids as far as solubility in liquids go: as temperature drops they are more soluble in liquids, hence a warm beer or warm fizzy drink goes flat quicker than a cold one)