Salvinorin A Extraction and Purification

[Loveall]

...if it were me i might try recrystallizing in boiling IPA

I did this and it seemed to work without having to do the hexane, water, and IPA washes. However, my yield is 115mg from 85g of dry leaves (1.35 mg/g), which is half of gibran2's yield (not sure why).

I started with an off green residue after drying a 3x freezer cold acetone extract. This was re-X ed twice in boiling IPA (see small white xtals growing in the first picture) to get an off white powder (second picture).

I was expecting more like 250mg of yield . Gonna dry the IPA re-x solution and see if there is something there. Also, it seems that light can degrade salvinorin in solution and I was not careful about that. Finally, my leaves were collected before a frost and the plants had been in cold weather for a few weeks (not sure if that matters).

 

[Brennendes Wasser]

Just to throw in, you can still benefit from Naphtha when just adding it while doing the re-x.

So if you are left with your chilled and evaporated Acetone pulls and you dissolve them in a minimal amount of IPA, then just add 2x the volume of Naphtha afterwards and place it in fridge, then after 5 min the grey voluminous clouds will have separated and settled even stronger, so you can speed up the process and also decant them much easier. With only that minimal amount of IPA it may be a little messy, but increasing the volume when its supposed to precipitate may make things much easier.

What is your idea for vaping?

Had no luck with hot plate setups and pure compound, but still there should be a more convenient and elegant way, than smoking this stuff dispersed on cigarette paper - thats what at least comes quite fast up while googling :/

 

[rOm]

What is your idea for vaping?

Had no luck with hot plate setups and pure compound, but still there should be a more convenient and elegant way, than smoking this stuff dispersed on cigarette paper - thats what at least comes quite fast up while googling :/

when I first joined the forum, I didn't have extracted DMT yet, but you'll see inmy first 10 posts, I was vaping pure isolated salvinorin A crystal.
I used simply a torch and a "light bulb" like vaporizer ( not homemade, byut the kind of glass pipe they sell in smokeshop, I found it neat for it, I did have the salvinroin A in 96° ethanol suspension so waslike 1Mg per ml, I would take my dose andlet it evap in the glass pipe, so when evapped I'd have my dose in the glass that I would then hit underneath with the torch. a bit how we vape 5 meo DMT toad secretion in most cases too.
Hope that helps. You can check the pipe used in a Vice mag about bufo alvarius and Gerry as practitioner use a cool piece of glassware I forgot the name but its a bit more fancy thanjust the glass bulb. I liked mine but did mess around with other extract more resinous and applied too much heat til it broke.
Was cheap and efficient enough with torch for salvinorin A.
Any oil burner like pipe willdo thejob. I use the GVG for salvia extracts too. but also got a cool wax ePen I could try, I know it works well for DMT and would likely workwith salvinorin too.

 

[physics envy]

What is your idea for vaping?

Here's a local topic on just that subject

Salvia Vaporization

 

[Loveall]

I tried to see if Salvinorin would dissolve in PG, but I could not get it to work. I added some ethanol, and even HPBCD with some water. I could not make an e-juice that would dissolve the salvinorin and did not succeed.

I think rOm's approach is the way to go. Perhaps using an acetone solution and e-mesh to remove it before taking the hit. I think one drop (20ml) of acetone can carry 0.5 mg of salvinorin, hopefully such a small amount evaporated quickly does not affect o-rings and such

 

[Loveall]

Just to throw in, you can still benefit from Naphtha when just adding it while doing the re-x.

So if you are left with your chilled and evaporated Acetone pulls and you dissolve them in a minimal amount of IPA, then just add 2x the volume of Naphtha afterwards and place it in fridge, then after 5 min the grey voluminous clouds will have separated and settled even stronger, so you can speed up the process and also decant them much easier. With only that minimal amount of IPA it may be a little messy, but increasing the volume when its supposed to precipitate may make things much easier.

Thanks Brennendes Wasser, I tried this out. I added naphtha to the used frezer re-X IPA and more stuff crashed, resulting in 65mg of very white powder. In my case it helped recover yields, perhaps I used too much IPA for re-X originally (I used ~ 50ml, which should only carry 30ml of salvinorin at room temp). The 65mg is more than I expected, maybe the plant stuff helps salvinorin dissolve in cold IPA (?) or maybe the product is not all salvinorin. Originally I let it crash for over a week, so it should have finished the IPA re-X before I added naphtha.

Overall, I think naphtha does make re-X easier and more robust. Also, it can be used to rinse off any traces of green IPA wich helps remove the small off-color to leave a white product that looks very nice.

The naphtha use brings the total yield up to 180mg for 85g of dry leaves, a respectable 2.1mg/g (assuming the powder is mostly salvinorin), but not at the level that gibran2 got with the washing approach on his leaves.

Next year, I'll do this again with my grow:

3x pulls (60s shaking) with(-15C) acetone pulls of dry leaves. Evap and pickup green powdery residue with boiling IPA. Add 2x volumes of naptha and crash in freezer over a few days. Decant and immediately wash with naptha to help remove traces of green solvent, finally dissolve in acetone and dry slowly in flat dish away from light. The result is a beautiful white xtaline powder, I'll report back with the bioassay someday.

Cheers.

 

[Loveall]

FYI, I went ahead and added a TEK for the cold acetone and IPA/Naphtha re-X process we worked on/tested in this thread.

Chilled Acetone with IPA and Naphtha re-X - DMT-Nexus Wiki

wiki.dmt-nexus.me

Cheers

 

[enthdimension]

3x pulls (60s shaking) with(-15C) acetone pulls of dry leaves. Evap and pickup green powdery residue with boiling IPA. Add 2x volumes of naptha and crash in freezer over a few days. Decant and immediately wash with naptha to help remove traces of green solvent, finally dissolve in acetone and dry slowly in flat dish away from light. The result is a beautiful white xtaline powder, I'll report back with the bioassay someday.

Cheers.

Hi, Thanks so much for this! How did this work out for you?

Did you think you still need to do a water wash before (or after) the boiling IPA/Naptha procedure?

 

[Loveall]

3x pulls (60s shaking) with(-15C) acetone pulls of dry leaves. Evap and pickup green powdery residue with boiling IPA. Add 2x volumes of naptha and crash in freezer over a few days. Decant and immediately wash with naptha to help remove traces of green solvent, finally dissolve in acetone and dry slowly in flat dish away from light. The result is a beautiful white xtaline powder, I'll report back with the bioassay someday.

Cheers.

Hi, Thanks so much for this! How did this work out for you?

Did you think you still need to do a water wash before (or after) the boiling IPA/Naptha procedure?

No water wash was needed for this process. Technique and source material can vary though. Best of luck!

 

[philleoNardo]

Everything I'm asking may be super obvious to the chemists on here, but what is the purpose of the naphthalene Naphtha wash?

I'm looking at this erowid tek. https://www.erowid.org/plants/salvia/salvia_extraction4.shtml


Also, even earlier in the process: Before the naphthalene Naphtha wash but after the shaking of the raw plant material in IPA, it seems there are several decantings of the liquid. Is the idea just that the heavy sediment will sink while the salvinorin A will rise, and by decanting over and over we can get a clearer and clearer solution that, when dried, will be more concentrated than the raw planet material?

Maybe related to all of this, in the erowid tek there is shown a waxy puck after the IPA wash but before the naphthalene wash. Is this...smokeable?

Motivating all of this is a concern about residual naphthalene naphtha after the extraction process still being in the extract, but even setting that aside, I'm just curious about understanding each step of the process better.

 

[Transform]

Everything I'm asking may be super obvious to the chemists on here, but what is the purpose of the naphthalene wash?

I'm looking at this erowid tek. https://www.erowid.org/plants/salvia/salvia_extraction4.shtml


Also, even earlier in the process: Before the naphthalene wash but after the shaking of the raw plant material in IPA, it seems there are several decantings of the liquid. Is the idea just that the heavy sediment will sink while the salvinorin A will rise, and by decanting over and over we can get a clearer and clearer solution that, when dried, will be more concentrated than the raw planet material?

Maybe related to all of this, in the erowid tek there is shown a waxy puck after the IPA wash but before the naphthalene wash. Is this...smokeable?

Motivating all of this is a concern about residual naphthalene after the extraction process still being in the extract, but even setting that aside, I'm just curious about understanding each step of the process better.

It literally says in the method you linked to:

What you will be doing is washing out most of the wax from the extract with the Naphtha, which Salvinorin isn't soluble in

Please note, naphthalene is an entirely different substance than naphtha. Do not attempt to use the former substance (which has been used in mothballs) for this process - only the latter will do. And sorry about trivial names in chemistry, but it would be ideal if you had some idea how adding or removing one or more letters to or from the end of a word makes it a different word, in most cases.

 

[philleoNardo]

It literally says in the method you linked to:

Thank you. This leads to my follow-up question in my post regarding the uses of the waxy output from the intermediate step.

 

[Transform]

Thank you. This leads to my follow-up question in my post regarding the uses of the waxy output from the intermediate step.

I'd say, if you have clean-evaporating naphtha it'll be less of a concern than smoking the crude IPA extract which is full of fats and chlorophyll, and looks like a great way of putting tarry degradation products into your lungs if you were to try smoking it. Careful vaporisation might yield marginally 'friendlier' results, but salvinorin A does require rather a high temperature to vaporise - high enough to start decomposing chlorophyll a bit.

Naphtha residues anyhow can be practically eliminated by recrystallising the washed sal A with ethanol or IPA, if you're really concerned about a few micrograms of alkanes.