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Skunks Myco Lab

SkunK

🥀𝕊ⲕü𝗻𝙆🥀
Joined
Jan 22, 2016
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Figured I’d share my mycology projects and musings here, while nothing here is ground breaking, this is just a place to share/document & work at advancing my mycological pursuits.



While I have been growing Mushrooms for a while, I haven't been able to progress very far with my mycological journey due to constant drama in my life inevitably throwing a wrench in the spokes to grind me to a halt quick fast. I’m hoping life decides to give me a brake from all the drama that's been a consistent part of my life the last few years. Luckily through my sporadic growing, I have been able to take care of m needs up until this point.
I don't have a dedicated lab, mine doubles / triples or even quadruples as a jack of all room, and probably isn't the cleanest of places but it has served me well!
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I've had great success working from spore prints, making spore syringe solutions, Making LC’s & basic agar work! I have had my greatest success making and fruiting BRF PF Cakes. So far it has treated me very well and I have been able to supply myself with more mushrooms than I have ever needed.
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I had started Micro dosing end of last year, and with the increased consumption with my close to daily needs; I need to start focusing on moving away from my tried tested and true PF styled grows and start transitioning in to bulk tub grows. I think I also need to start moving away from BRF/Rice in general and focus on some other grains to utilize for my bulk tub/shoebox grows.
(Jack Frost)
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(Golden Teacher)
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(PES Hawaiian)
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Currently I am exploring and experimenting with Brown Rice/Pop Corn and now moving onto Rye Grains ( found a cheap source for these grains at the local feed store), which makes it really appealing. I’ve only just yesterday started to proceed with this grain and picked up 2 50 LBS bags from the feed store yesterday afternoon, and working on running them through the PC this afternoon.
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I’ll use this thread to document and share all the aspects of my grow so as not to post multiple threads and keep everything related to my grows and Mycology work here in one place.
Spores.jpg
Feel free to jump in the chaos , comment , offer advice, opinions and what ever banter you want to bring to the thread

I was thinking of taking some of my PF Cakes to bulk, but seeing as I’m low on supplies till I can grab some more things end of month, and what I have is already allocated to other projects, I’ll just continue to fruit off the cakes I have, and continue to harvest enough fungi for my needs for the time being.

What I’m kind of working with at the moment is:

45 BRF jars ranging from 30% - 100% colonized Mostly 1/2 Pint Jars:
ShotgunTub.jpgShotgunTubHaulta.jpg
8 - Huautla
5-Revert Melmac
2- B+
5- B+ Leucitic
5- Thailand Lipa Yai
15- South American
3- Cambodian
1- PES Hawaiian
1- Jack Frost
4- Ecuador
1- Enigma (LC)

Whole Brown Rice - 6 Quarts Total:
2 Quarts - Enigma (LC’s Inoculated)
2 Quarts - African Transkei
2 Quarts - B+ Leucitic
Rice-Enigma3.jpgRice-Enigma5.jpg


Pop Corn 3 Quarts Total:
1 Quart - Enigma
1 Quart - African Transkei
1 Quart B+ Leucitic

Liquid Cultures:
-Enigma
-B+ Leucitic
-PES Hawaiian
-African Transkei

Prepping a few more LC jars this evening of some (South American , Cambodian, Huautla , Revert Melmac , Jack Frost)
LC's.jpgLC's1.jpgLC'sEnigma.jpgLC'sAfricanTransekie.jpg


Rye Grain:
I’ve got 8 Quarts I’m working at running through the PC and Instapot this evening and will work on inoculating tomorrow with some liquid Culture.
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Agar Work:

I’ve also got 110 - 125ml Canning jars that I have been cycling through playing around with some agar work, while they are nice and cheap, it makes looking at your agar and growth problematic, but they were cheap, can be reused and have been fun learning on. I have ordered 40 borosilicate glass petri dishes 100x15mm , although shipping is 3-4 weeks to the door so have some time till they arrive.

I also have a few hundred 100x15mm plates I will begin to work through ... well have to practice pouring these disposable plates , as my last foray with agar a few years back didn’t go even remotely acceptable so I'm sure I will have a learning curve here getting the hang of it and getting some successfully poured agar plates.

Agar.jpgAgar1.jpgagardishHuautla1.jpg

This thread might get picture heavy, I'll try to keep a majority of the photos embedded into the post as thumbnails
 
I was working a little late last night, trying to get all my Rye Grains ready and PCed, and I think I over hydrated my 2nd batch of grain. i ended up with a lot of burst grains after my simmer. I have picked out most of the burst grains (as best I could) and decided to send it through the PC and will Inoculate once my gardens and fans go out later this afternoon!

I have read and seen people say that burst grains lead to contamination, I've also been told it shouldn't be a problem and to just send it.

Anyone more experienced with Rye grains wanna chime in with their opinions and experience?
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I have another question I'm looking for opinions on, when using Agar Wedges to inoculate grains, how much/many mycelium wedges does one add to a quart jar. would a whole plate minus a transfer or two be adequate ?
 
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