Grasshoppers
Established member
Preamble
Grasses, enduring through the ages, have flourished upon the earth, unravelling the fabric of reality to reveal the underlying essence of the cosmos. With each seed sown and every plant nurtured, nature's mysteries unfold before us, guiding us toward a promising tomorrow.
Within this discourse, we shall explore our methodology for selecting and cultivating Phalaris, offering our insights and expertise to the psychedelic community. As our plants take root and flourish, we hope they will emerge as beacons of progress, illuminating the path forward by dispelling the shadows of ignorance and ushering in enlightenment.
Project Methodology Overview
The project methodology encompasses the systematic selection, controlled breeding, and cultivation of Phalaris specimens sourced globally. This selection process is facilitated through Thin-Layer Chromatography (TLC) and bioassay techniques for the identification of desirable traits.
Selected specimens undergo controlled breeding procedures, aimed at consolidating and enhancing the desired genetic characteristics. Through deliberate mating, the genetic pool is manipulated to produce offspring with optimal traits.
Subsequently, the cultivated offspring undergoes rigorous selection process to further refine desirable attributes, ensuring the propagation of superior genetic material.
In light of our commitment to inclusivity and accessibility, it is imperative that our research methods remain practical and cost-effective. By utilizing easy-to-use and readily available techniques, we ensure that individuals from diverse backgrounds can actively participate in the project.
A key aspect in this programme involves the creation and dissemination of new phalaris breeds consolidated as cultivars to the psychedelic community. By sharing these plants, we aim to facilitate collaborative research endeavors and foster innovation.
Current Project Status
The project has progressed to a stage where a multinational selection of promising Phalaris aquatica strains has been identified through TLC, indicating their potential as candidates for the breeding program. Clones of these selected plants have been taken for breeding purposes. Some of these clones have already been dispatched to participating breeders, where they are currently undergoing the recovery process. Meanwhile, the cloning process for the remaining plants is still underway. The initial breeding cycle is expected to commence during the upcoming summer season.
Key Findings
Contribute
We welcome individuals who are willing and capable of supporting the breeding project. At its current stage, you can contribute if:
Open Research Topics
Supplementary Material
Grasses, enduring through the ages, have flourished upon the earth, unravelling the fabric of reality to reveal the underlying essence of the cosmos. With each seed sown and every plant nurtured, nature's mysteries unfold before us, guiding us toward a promising tomorrow.
Within this discourse, we shall explore our methodology for selecting and cultivating Phalaris, offering our insights and expertise to the psychedelic community. As our plants take root and flourish, we hope they will emerge as beacons of progress, illuminating the path forward by dispelling the shadows of ignorance and ushering in enlightenment.
Project Methodology Overview
The project methodology encompasses the systematic selection, controlled breeding, and cultivation of Phalaris specimens sourced globally. This selection process is facilitated through Thin-Layer Chromatography (TLC) and bioassay techniques for the identification of desirable traits.
Selected specimens undergo controlled breeding procedures, aimed at consolidating and enhancing the desired genetic characteristics. Through deliberate mating, the genetic pool is manipulated to produce offspring with optimal traits.
Subsequently, the cultivated offspring undergoes rigorous selection process to further refine desirable attributes, ensuring the propagation of superior genetic material.
In light of our commitment to inclusivity and accessibility, it is imperative that our research methods remain practical and cost-effective. By utilizing easy-to-use and readily available techniques, we ensure that individuals from diverse backgrounds can actively participate in the project.
A key aspect in this programme involves the creation and dissemination of new phalaris breeds consolidated as cultivars to the psychedelic community. By sharing these plants, we aim to facilitate collaborative research endeavors and foster innovation.
Current Project Status
The project has progressed to a stage where a multinational selection of promising Phalaris aquatica strains has been identified through TLC, indicating their potential as candidates for the breeding program. Clones of these selected plants have been taken for breeding purposes. Some of these clones have already been dispatched to participating breeders, where they are currently undergoing the recovery process. Meanwhile, the cloning process for the remaining plants is still underway. The initial breeding cycle is expected to commence during the upcoming summer season.
Key Findings
- In Phalaris aquatica, a diverse array of alkaloid profiles has been observed. 5-MeO-DMT proved prevalent amongst the tested specimens at varying concentration (TLC semiquantitative analysis), whereas N,N-DMT was only detected in few selected strains with a significant portion of the tested samples exhibiting minimal or no detectable DMT.
- Notably, 5-MeO-DMT appears to be particularly sensitive to high concentrations of NaOH (50% concentration was tested) during the extraction process.
Contribute
We welcome individuals who are willing and capable of supporting the breeding project. At its current stage, you can contribute if:
- You have expertise in TLC or LC-MS; we have numerous plants awaiting testing.
- You possess knowledge of pollen collection and application procedures with Phalaris species.
- You can provide Phalaris strains or clones that have been confirmed to exhibit psychedelic properties through bioassays.
- You are capable of investigating open research topics.
Open Research Topics
- Exploration of alternative extraction Solvents: Investigating wide spectrum solvents suitable for extracting alkaloids from Phalaris beyond Chloroform and Dichloromethane in persuit of cleaner extracts for higher TLC resolution and easier handling.
- Refinement of Extraction Processes: Determining the optimal method for cleaning extracts. SPE (solid phase extraction) cartridges are an example but price may be counterproductive.
- Enhancing TLC separation of closely related tryptamines: Researching eluents to improve the distinction of N,N-DMT from 5MeO-DMT.
- Exploration of environmental factors, including daytime and seasonal variations, on alkaloid concentration and composition.
- The effects of SiO2 supplementation on the concentration of N,N-DMT and 5-MeO-DMT and other alkaloids in high yielding clones.
- To catch the changes in alkaloid levels throughout the lifecycle of Phalaris, carry out periodic testing on carefully chosen mature plants over a period of at least one year. This extensive duration will allow us to capture the nuanced fluctuations in alkaloid levels within the selected plants, thereby enhancing our understanding of their dynamic nature.
Supplementary Material
- Practical Approach to TLC Analysis of Phalaris Alkaloids
- Phalaris Identification guide: Coming soon
- A bioassay guide: Coming soon
Attachments
Last edited: