Quote from "Solid substrate mediated changes in ergot alkaloid spectra in solid state fermentation system" (Hernandez, 1992)
Wheat or rye grains were cut into 3 pieces and moistened with distilled water to 50% moisture level for autoclaving at 121 C for 15 min. The lumps formed were broken to separate the grain particles for drying at 60 C for about 24 h. The grains thus processed can be stored upto 6 months without any contamination.
Liquid nutrient' medium which was used for moistening the processed grains or impregnating rocessed sugar cane pith bagasse contained (g*l-I ): ammonium oxalate 9.6, urea 1.73, KH2P04 0.625, MgSO4 * 7 H20 O.O25,ZnSO4 * 7 H20 0.01, and NH4OH to raise the pH to 5.2
The medium was sterilized at 121 C for 20 min and inoculated with spores. Wheat or rye grains (40 g) were mixed with 60 ml of the inoculated liquid medium to get 60% moisture in the moist solid medium.
60 g moist medium was charged in static column fermenter of size 20 cm length x 4 cm diameter. The columns were aerated at a rate of 4 1 humidified airh-l per column and the fermentation was carried out at 26 C for 10 days
...
In contrast to the nearly equal production of total alkaloids by Claviceps purpurea 1029c on three different solid substrates in SSF system, the spectra of alkaloids produced on these substrates differed significantly (Table 1). For example, ergonovine was the major alkaloid formed and accounted for 93.91% of the total alkaloids in the wheat grain medium. The concentrations of ergotamine and lysergic acid derivatives, in this case, were >0.5% while that of lysergol was 5.18% of the total alkaloids formed. In contrast, lysergic acid derivatives and ergonovine together accounted for 98.78% of total alkaloids formed in rye grain medium, their ratio being 1:0.48 (Table 1). Lysergol accounted for 1.14% of total alkaloids while ergotamine was present in traces in the rye grain
medium.
