Extraction of Angico stem bark

[modern]

So for the first attempt I did 25g of Yopo stem bark extracting with hcl then alkaline with lye and heptane pulls.

I tried to defat but just caused emulsions due to lots of saponins. Then shook the heptane and lost like 10-20% heptane as well.

I was expecting 10-100mg based on older reports found in simple tryptamines and paper I found but maybe got 1mg? This is Benzoate salt of whatever was pulled.

I was gonna try oral with maoi but may just try to do crude TLC since so little amount.

The correct species for 5meo should be Anadenanthera peregrina and others may just be weaker

Reattempting with 175g and slower and longer pulls. I’ll report back in a month or so when I finish up. Also whenever I get around to do my TLC

 

[modern]

So on the second attempt I added lye slowly and got ph 11-12. Rather than grey color that DMT bark gets I got a purple color. However after a day of gentle swirling when I salted it there was zero clouding. So I increased the ph again to 14 and the color became black. I added the heptane again and will attempt salting again…

So unless slight excess hcl and excess lye breakdown the 5meo this time I should get a measureable yield since used 175g starting material.

Next attempt I’ll use much lower hcl content or maybe another salt then try ph 11 again. To see if hcl is to blame. I used hcl first round but not prolonged time. Zero heat used.

 

[modern]

at ph 13-14 the heptane pull did cloud after salting benzoic acid. so idk if 5-meo is the alkaloid that is pulled or not. Maybe something else... I'll do a small bioassey in the future. After a finishing the complete extraction I'll share the yield I got of whatever is being salted here... lol if it is just gramine.

 

[modern]

so I'm still pulling and these are yielding more (still tiny amounts) of whatever alkaloid the heptane pulled. Using benzoic acid and only swirling to prevent emulsions.

Like I mentioned used HCL to pull from bark (still soaking for more pulls) then added excess lye to ph 13-14. some say that 5-meo-dmt is sensitive so IDK if this is actually DMT or another.

I'm doing water washes of this beaker and evaporating all together...

 

[modern]

the first 2 pulls have been dried collectively and here is the resulting benzoate. I have 1 more acid pull to do of the bark material to complete in the future. Maybe if I used heat may have had better yield but decided against it this attempt. At least it yielded something even if lo content.

I'm unsure if I should try it or attempt my crude TLC...

 

[Varallo]

That would be a very impressive result if this indeed turns out to be the target molecule. I would strongly recommend running a TLC to get a quick indication. If you have your own standard of 5-MEO-DMT available, the comparison should be straightforward: simply run your extracted sample alongside the standard and compare the Rf values.

Since this is only intended as a rough screening, the setup does not need to be elaborate. Even a very simple paper-based run could provide a preliminary indication of whether the compound you are looking for might be present. That said, a standard silica TLC plate will give cleaner separation and more reliable results.

Good luck

 

[modern]

That would be a very impressive result if this indeed turns out to be the target molecule. I would strongly recommend running a TLC to get a quick indication. If you have your own standard of 5-MEO-DMT available, the comparison should be straightforward: simply run your extracted sample alongside the standard and compare the Rf values.

Since this is only intended as a rough screening, the setup does not need to be elaborate. Even a very simple paper-based run could provide a preliminary indication of whether the compound you are looking for might be present. That said, a standard silica TLC plate will give cleaner separation and more reliable results.

Good luck

I'll do my DIY silica tlc plate and I have a 270nm uvlight so it should glow green when dry.