Very interesting. They did an extraction with xylene and lead(II) acetate.
It also describes oral bioassay:
I can help if you need to translate any section, Portuguese is similar to Spanish so I understand it well.
It also describes oral bioassay:
One of us that spontaneously ingested 0,04g nigerine had the opportunity to experience heart rate acceleration (90), a feeling of enhanced hearing, light nausea, and respiratory symptoms (light dyspnea).
I can help if you need to translate any section, Portuguese is similar to Spanish so I understand it well.