I have phyllodes from acacia acuminata narrow phyllode strain grown in canada.
endlessness said:Im not sure about your specific test and what happened there but I assure you they work very well and any mislabelling issue that has happened in the past was a rare exception, and I appologize in their name for whatever may have happened. .
As for whoever asked before, a simple tea and evap would not be good enough, you'd need to redissolve it in methanol or ethanol or something and filter that properly and evap again, or do an STB or A/B.
pinkoyd said:endlessness said:Im not sure about your specific test and what happened there but I assure you they work very well and any mislabelling issue that has happened in the past was a rare exception, and I appologize in their name for whatever may have happened. .
I know they were very busy and with equipment breakdowns and such they were struggling to get their paid customers taken care of. At least that's what I was given to understand. That said, it took over a year for them to get all the samples tested and the results back to me. As an example, I had five different Psychotria viridis named clones, including the Nexus hybrid, all carefully and plainly labeled. All I got back was a spreadsheet with a list of "Psychotria Sample #1" "Sample #2" and so on, so I don't know which was the Nexus clone which was the one I was particularly interested in. All I can say it is on a par with viridis, but I can't be any more specific than that.
Plus they only listed the dominant alkaloid, so while I was interested if DMT actually showed up in a Mucuna pruriens sample all they listed was L-Dopa.
There were other issues that I won't go into, but I think the takeaway here is not to overwhelm them with too big a box of goodies.
Don't be discouraged Endlessness. I'm sure there are many who wish to participate but, aren't in a position to send any samples in an expedient manner.endlessness said:The offer still stands, can't believe not more people are taking advantage of freely getting their plants and extracts GC/LC-MS tested
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endlessness said:The offer still stands, can't believe not more people are taking advantage of freely getting their plants and extracts GC/LC-MS tested
Plantatron and everyone else, just get the samples ready for shipment and pm me and I'll send the lab address![]()
The GC/MS is an Agilent 7890B gas Chromatograph, coupled to a 5977A quadrupole mass spectrometer detector. The gas chromatograph was fitted with G4513A auto sampler injector. Insert liners packed with silanized glasswool were used, and the injector and the interface were operated at 280C. Samples were injected in split mode into a 30 m 0.25 mm i.d., 0.25 mm film thickness 5% phenylmethylsilicone column (HP-5MS, Agilent Technologies). Helium was used as carrier gas at a flow rate of 1 mL/min. The oven temperature was initially maintained at 90C for 2 min and programmed to reach 320C at 20C/min. It was finally maintained at 320C for 9.5 min (total run time was 21.5 min). The mass spectrometer was operated in electron impact ionization mode at 70 eV.
LC-MS/MS: liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) using an Agilent 1100 series, HPLC chromatograph (Agilent Technologies, Palo Alto, USA) and an Esquire 3000 plus mass spectrometer MRM (Bruker Daltonic GmbH, Bremen, Germany). Chromatography was performed using a Poroshell 120 EC-C18 column (100-mm length 2.1 mm i.d., 2,7 mm particle size) at 30 8C. The mobile phases consisted of 1% formic acid (A) and 1% formic acid in methanol (B). The following gradient elution was used: at time 0 min 15% B changed to 90% B in 7 min, held for 1 min, changed back to the initial conditions in 1 min. Before injection of the next sample the column was allowed to re-equilibrate during 7 min.
The flow rate was 0.35 mL/min. The electrospray source (ESI) was operated in the positive ionization mode. Product ions that were obtained by collision-induced dissociation allowed the MS/MS to be operated in the multiple reaction monitoring (MRM) mode. The MRM transition was 371 (precursor ion) to 188 (product ion). The dwell time was set at 0.25 s. The desolvation gas was nitrogen set at 365C and delivered at a flow rate of 9 L/min. The capillary voltage was 3.90 kV, and the collision gas was helium. Bruker Compass Hystar system software Version 3.2-SR2 was used for instrument control and identification.