Quantitative and Qualitative Analysis by HPLC of Major Peganum harmala Alkaloids at Different Stages

[entheogenic-gnosis]

A very informative article involving the development of harmala alkaloids during different stages of growth of the peganum harmala plant and development and concentrations of harmala alkaloids in different areas of the plant.

Quantitative and Qualitative Analysis by HPLC of Major Peganum harmala Alkaloids at Different Stages of Development | Mayad | Journal of Natural Sciences Research

-EG

 

[DansMaTete]

Very interesting link, thanks.

It means it's possible to extract from the whole plant (specialy the roots) as it seems mine don't want to grow properly (why, i'd like to know !) and they will never produce seed.

 

[entheogenic-gnosis]

Interesting,
I've never grown peganum harmala, but feel I could, I hear the plant grows wild in New Mexico, it may even be considered an invasive species there, any way I live in Colorado and feel the plant could grow here out doors, but have not tried thus far, and probably won't any time soon.

I've thought about sprouting some seeds for an indoor plant as an experiment, but again have just been to busy to ever do it.



-EG.

 

[DiMoiTou]

Cool link. :thumb_up:
Could anybody explain the low yield from seeds found in this analysis?

The total content of these major alkaloids in seeds is then approximately 1.22%

That's very far from the usual 5% observed around here.

 

[endlessness]

Ive had seeds that yielded 1%. I'd just write it off as natural variability.

 

[DansMaTete]

And this study quotes an other study :
"Ben Salah et al. (1986) reported that alkaloid content rises sharply in summer, during the ripening phase of fruit at harvest of the seed and they concluded that the rate of alkaloids is much higher in the seed (3-4%) than in the root,..." which is closer to 5%

Endlessness, did you grow those 1% seeds or did you get them from someone ? (i'm just looking for advice from another grower )

 

[endlessness]

I got sent them by a friend. Mine didn't survive, I think it rained too much and the seedlings are sensitive to excess water.

I'll try growing again, will start germinating soon

 

[Auxin]

Thats ironic since my first success at growing it was planting in pH 8.2 soil to which I mixed in pure limestone powder :lol:
All my subsequent attempts were just the straight pH 8.2 loam, low organics. All were successful.
The only direct seeded plants that ever died in that soil were right next to a black walnut tree, I suspect P. harmala is juglone intolerant.

Interesting paper. I wonder why they chose to ignore the quinazoline alkaloids. Seasonal variation of quinazoline versus ß-carboline in the roots would be interesting to know, as many people roughly judge adequate dose by the onset of ataxia caused by the other alkaloids.