Hey nexians. Haven't been around much lately but decided to summarize some of the imaginary theoretical work SWIM has thought up about jungle spice. I'll avoid the technical details and summarize the results as simply as possible.
Anyway here is the imaginary information:
SWIM has taken a number of analytical approaches analyzing the substance extracted from mimosa hostillis known as jungle spice. SWIM has compared mimosa hostillis extracted with nothing but methanol (cold for a week in dark) and jungle spice prepared by A/B and STB methods usually employing acetic acid as acid and toluene as organic solvent. SWIM has also cleaned up jungle spice multiple times with hexane and reanalyzed the material.
The analytical approaches used have been: HPLC with UV (PDA) detection and GC-MS.
When one analyzes mimosa extracted cold with methanol by HPLC 3 compounds are apparent with very similar UV spectra. When compounds have the same (or very similar) UV spectra it means they have a similar chromaphore which means there is some aspect to their structure that is very similar. Anyway one of these compounds is DMT, the other is yuremamine, and one other compound is present that SWIM thinks is a beta-carboline or NMT (See MS discussion). The ratio is something like this dmt (66%), yuremamine (28%) and other alkaloid (6%).
When one analyzes jungle spice made by STB or A/B methods two main compounds are visible. DMT and the other alkaloid. There are some very very minor peaks but basically thats the majority of whats observed. Ratio is about 95% DMT and 5% other alkaloid.
When one analyzes jungle spice by GC-MS DMT is the major compound. Two other compounds can be observed NMT and 2-methy-1,2,3,4-tetrahydro-beta-carboline. However since some compounds are not analyzable by GC because they are not volatile SWIM decided to derivitize the extract with a trimethylsilyating (TMS) reagent and analyze it again. By using N-Methyl-N-trifluoroacetamide (MSTFA) with 1% trimethylchlorosilane (TMCS) this reagents reacts to interchange protons on functional groups like OH (also works on amines etc many functional groups) with a trimethylsilicon (TMS) group. Derivitizing with these reagents has a number of advantages for analysis on GC. It improves thermal stability of compounds, it improves their volatility (by reducing intramolecular forces such as hydrogen bonding on functional groups), it also can improve chromatographic separation, and finally it aids in structure elucidation.
Anway if one analyzes jungle spice after MSTFA + 1% TMCS derivitization the following can be observed. DMT and its TMS derivatives, that beta-carbolines TMS derivative, a few organic acids at least one fatty acid and at least one phenolic compound. A number of small amines and indole like structures are also present but these are most likely artifacts of the reaction (thats normal).
Finally the conclusion from all these experiments is that jungle spice is mostly DMT! SWIM has said this before but after following up these experiments with newer experiments and other approaches SWIM firmly believes the psychoactivity of this extract is mostly from DMT. The increase in potency could be explained many ways but all would be speculative. Maybe there is potentiation (although I doubt it because of low concentrations of other alkaloids) or maybe DMT vaporizes better for some reason when its in jungle spice.
The difference in color and consistency of these extracts probably has much to do with varying ratios of the alkaloids and other compounds like fatty acids and organic acids that were observed. The so called differences in effects compared with DMT are probabaly largely subjective or dose related but again thats speculative.
Basically when people say jungle spice is "another alkaloid" they are wrong. Its DMT. This is important for those who extract it to realize because it means there is a lot more DMT still present in your extract. SWIM has cleaned up the extract 3 times with hexane and DMT is still present. SWIM will try and fully exhaust the material of DMT and analyze again but that might be very difficult if not impossible without chromatography.
Anyway here is the imaginary information:
SWIM has taken a number of analytical approaches analyzing the substance extracted from mimosa hostillis known as jungle spice. SWIM has compared mimosa hostillis extracted with nothing but methanol (cold for a week in dark) and jungle spice prepared by A/B and STB methods usually employing acetic acid as acid and toluene as organic solvent. SWIM has also cleaned up jungle spice multiple times with hexane and reanalyzed the material.
The analytical approaches used have been: HPLC with UV (PDA) detection and GC-MS.
When one analyzes mimosa extracted cold with methanol by HPLC 3 compounds are apparent with very similar UV spectra. When compounds have the same (or very similar) UV spectra it means they have a similar chromaphore which means there is some aspect to their structure that is very similar. Anyway one of these compounds is DMT, the other is yuremamine, and one other compound is present that SWIM thinks is a beta-carboline or NMT (See MS discussion). The ratio is something like this dmt (66%), yuremamine (28%) and other alkaloid (6%).
When one analyzes jungle spice made by STB or A/B methods two main compounds are visible. DMT and the other alkaloid. There are some very very minor peaks but basically thats the majority of whats observed. Ratio is about 95% DMT and 5% other alkaloid.
When one analyzes jungle spice by GC-MS DMT is the major compound. Two other compounds can be observed NMT and 2-methy-1,2,3,4-tetrahydro-beta-carboline. However since some compounds are not analyzable by GC because they are not volatile SWIM decided to derivitize the extract with a trimethylsilyating (TMS) reagent and analyze it again. By using N-Methyl-N-trifluoroacetamide (MSTFA) with 1% trimethylchlorosilane (TMCS) this reagents reacts to interchange protons on functional groups like OH (also works on amines etc many functional groups) with a trimethylsilicon (TMS) group. Derivitizing with these reagents has a number of advantages for analysis on GC. It improves thermal stability of compounds, it improves their volatility (by reducing intramolecular forces such as hydrogen bonding on functional groups), it also can improve chromatographic separation, and finally it aids in structure elucidation.
Anway if one analyzes jungle spice after MSTFA + 1% TMCS derivitization the following can be observed. DMT and its TMS derivatives, that beta-carbolines TMS derivative, a few organic acids at least one fatty acid and at least one phenolic compound. A number of small amines and indole like structures are also present but these are most likely artifacts of the reaction (thats normal).
Finally the conclusion from all these experiments is that jungle spice is mostly DMT! SWIM has said this before but after following up these experiments with newer experiments and other approaches SWIM firmly believes the psychoactivity of this extract is mostly from DMT. The increase in potency could be explained many ways but all would be speculative. Maybe there is potentiation (although I doubt it because of low concentrations of other alkaloids) or maybe DMT vaporizes better for some reason when its in jungle spice.
The difference in color and consistency of these extracts probably has much to do with varying ratios of the alkaloids and other compounds like fatty acids and organic acids that were observed. The so called differences in effects compared with DMT are probabaly largely subjective or dose related but again thats speculative.
Basically when people say jungle spice is "another alkaloid" they are wrong. Its DMT. This is important for those who extract it to realize because it means there is a lot more DMT still present in your extract. SWIM has cleaned up the extract 3 times with hexane and DMT is still present. SWIM will try and fully exhaust the material of DMT and analyze again but that might be very difficult if not impossible without chromatography.
