Research The nexian phalaris breeding programme

[albertfive123]

The alkaloid concentration and composition of Phalaris aquatica can appear quite erratic.

Season, soil, and climate all influence the alkaloid profiles, and the interactions are complex and heterogeneous. Some plants fluctuate strongly, while others maintain a stable and potent profile across repeated tests. One thing we haven’t observed, though, is a plant switching between DMT and 5-MeO-DMT dominance.


This has direct implications for how we approach selection:

• All plants are tested, and the weakest 50% are discarded
• The process is repeated two more times with the remaining plants

As a result, any plant that makes it into the breeding line has been tested 3 times. This strongly favors individuals with both high yield and stable profiles.

The data collected during this process could eventually help us better understand these fluctuations. For now, though, most of the testing capacity is focused on selective breeding.

This season is going very well so far—I’ll post an update soon.

look u know what i think soli and climat test are great idea. i am gardener for a living and i wanna know more. can u tell me specific what soli and climate surcomestances u want to try ?

 

[Transform]

look u know what i think soli and climat test are great idea. i am gardener for a living and i wanna know more. can u tell me specific what soli and climate surcomestances u want to try ?

<Ahem>

https://forum.dmt-nexus.me/members/grasshoppers.65308/about

I'd say those guys are already pretty serious about this

 

[alpharex]

so how close are we to usable plants?

 

[Transform]

so how close are we to usable plants?

Research Thread 'The nexian phalaris breeding programme'

Apr 11, 2024

Preamble
Grasses, enduring through the ages, have flourished upon the earth, unravelling the fabric of reality to reveal the underlying essence of the cosmos. With each seed sown and every plant nurtured, nature's mysteries unfold before us, guiding us toward a promising tomorrow.

Within this discourse, we shall explore our methodology for selecting and cultivating Phalaris, offering our insights and expertise to the psychedelic community. As our plants take root and flourish, we hope they will emerge as beacons of progress, illuminating the path forward by dispelling the...

• Grasshoppers
• Replies: 124
• Forum: Collaborative Research Project

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[Grasshoppers]

Enhancements in Thin-Layer Chromatography (TLC) Protocols

This season’s workflow saw several key upgrades to our TLC testing, focusing on sample prep efficiency and high-precision digital analysis. We shifted toward a more robust extraction method and integrated custom hardware for both sample loading and data acquisition.

1. Sample Preparation & Extraction

To ensure a high concentration of target analytes, we refined the extraction ratio and alkaline environment.

• Protocol: 25 mg of desiccated leaf tissue is submerged in 1.0 mL of Methyl tert-butyl ether (MTBE).
• Alkalization: 3 drops of aqueous ammonia are added to facilitate the release of free-base alkaloids.
• Duration: Following a period of vigorous mechanical agitation, the samples are left to macerate for 8 hours to reach extraction equilibrium.

2. Precision Spotting

We moved away from manual capillary spotting to achieve better resolution and reproducibility.

• Hardware: A custom piezoelectric TLC loader was developed, utilizing a modified piezo buzzer as the micro-dispensing head.
• Result: This allows for the application of narrow, uniform "lines" rather than erratic spots, significantly reducing band broadening during development.

3. Chromatography Parameters

We opted for a straightforward, high-polarity mobile phase to suit our specific chemical targets.

• Stationary Phase: Unmodified 60Å Silica Gel (non-fluorescent) TLC plates.
• Mobile Phase: Isopropanol (IPA) and 25% aqueous ammonia in a 12:1 ratio.
• Method: Ascending development in a saturated chamber.

4. Digital Imaging & Fluorescence Induction

The most significant leap was in our detection and quantification setup. By bypassing standard consumer cameras, we gained much better raw data control.

• Sensor Logic: Plates are imaged using an IMX462 back-illuminated sensor. This sensor is particularly effective due to its high near-infrared (NIR) sensitivity and low noise floor.
• Processing: Raw data is pulled directly from the Bayer array via MIPI-CSI onto a Raspberry Pi, allowing us to process the signal without aggressive compression artifacts.
• Excitation: Dual-wavelength UV LEDs are used to induce fluorescence:
  
  • 275 nm: For short-wave excitation of primary aromatics.
  • 365 nm: For long-wave fluorescence of conjugated systems.

Exemplary Plate Analysis:

wet plate exposed to 275nm

dry plate exposed to 275nm