a theory..

[etherealsamba]

I have a different theory.

DET, nn--di-ethyl-tryptamine

DET has ethyl groups attached to its nitrogen atom, and that renders monoamine oxidase as unable to degrade it.

Diethyltryptamine, unlike DMT, is active orally at ranges of 50–100 mg, without addition or aid of MAOI, and is easily absorbed by the brain.

Now, in theory, det would be the de-methylation of DMT.
There would be 2 ways to demethylate, using a demethylation agent, or using an organism (an enzyme).

Now why don't we look into a little bit of folktale?

Apparently, friends and loved ones in communites that had plenty of acacia trees, would be buried near acacia tree.
one enzyme, 4-amino-1-β-D-ribofuranosyl-1,3,5-triazin-2(1H)-one is commonly used as a demethylating agent.

commonly availabe, today, yet is an analogue of cytidine, a nucleoside present in DNA and RNA.

could it be, that when their loved ones died, these enzymes in the fallen bodies were taken into the dirt, only to affect the tree they were buried near?
Could the enzymes present in the human body's dna actually have affected the growth and alkaloid production in the holy acacia tree?



now, for another idea.

Take to the Tenakh, the writing of ancient Ibrit (Hebrew)

Moses led his people Yshrael through the "desert" (actually translated more along lines of "wild" or "plains"), encounters the Elehym, who gave him details to build an ark of acacia wood. Later on we find out, that the ark would be to contain the 2 things made of rock that had been written by the hands of the Elehym; the Decalogue (Ten Words or Ten Terms).

The ARK, would along with the decalogue, contain the holy manna, which had rained down from heaven as food for Yshrael.

you might have read or heard that the "manna" was mushrooms, but the question is, what kind of mushrooms? Did they contain Tryptamines? Were they tripping out in the desert, with no other viable food? Or were these edible, non-active mushrooms that they were living on, day by day?

"Demethylation is the chemical process resulting in the removal a methyl group (CH3) from a molecule ...often catalyzed by an enzyme such as one of the Cytochrome P450 (CYP) family of liver enzymes and by fungal aromatic peroxygenases such as Agrocybe aegerita peroxygenase"


Agrocybe mushrooms, a few are poison, but there are plenty edibles in the agrocybe genus.

"The mushrooms in Agrocybe have brown spore prints and are small to medium-sized, saprobic species that grow in grass, wood chips, dung, garden mulch, or in woods--either terrestrially, or from deadwood. They are not subject to rapid decay (in contrast to the mushrooms in Bolbitius), and the caps, with a few exceptions, are dry. Unlike species of Conocybe, Agrocybe species have convex to flat caps."

Note, the areas these mushrooms grow, in "grass, woodchips, dung ( yisrel brought their cattle with them), garden mulch, or in the woods".

Now, the common misconception is that they were lead through the "desert" but the term loosely applies as "wild, wilderness, or plains"

Now, combined with the demethylation theory, and the theory, that these people had to have a viable food to keep them healthy enough to keep traveling, my proposition is that they were actually grubbing on edible mushrooms, that are considered edible today, ones that aren't considered "psychoactive" (no psylocibin->psylocin)

or there is always the theory that the manna they ate, were actually psychoactive mushrooms.

Owe to the idea, the description of the manna is "as the color of corainder seed" (cilantro seed )

the color of cilantro seed, is very much similar to the range of colors of psylocibe mushroom caps, as well as being very much like that of a few of the pictures of agrocybe genus..

either way, I firmly believe that any "bread" that could rain down from "heaven" (the sky) would be attributed as mushrooms. The spores even travel by air (through heavens).

Now, my idea about demethylation of DMT into DET at the area we have come to, is that the container known as the ark, was actually a grow box for keeping these mushrooms alive..

Either, the manna in the box was psylocibe genus, and the acacia wood was keeping a certain resonance that made these mushrooms a nice little personal stash (higher resonance, maybe even some dmt and other tryptamiens breaking down in the substrate, due to the acacia wood container)...

OR

the container contained agrocybe (or psilocybe even) mushrooms, and being contained for long enough, would de methylate the whole dmt content of the container itself (made of acacia wood) rendering any actives in the wood into DET (or DPT or DiPT etc)

In any context, i think that the ark of the covenant, whether literal or metaphorical, represents how living organisms, in this case mushrooms ( active or edible) and tryptamine plant material (Acacia) can react with eachother, symbiotically altering something in each other, or even one, that is not found in nature, but in the end, is completely active orally and plausible as a product of human intuition, or divine intuition given to man.

Breakdown of DMT -> DET using active cultures of mushroom enzymes.

 

[Infundibulum]

This whole thing is too far fetched and also incorrect at some important points.

Now, in theory, det would be the de-methylation of DMT.
There would be 2 ways to demethylate, using a demethylation agent, or using an organism (an enzyme).

This is the biggest mistake. Albeit a nice view to see things, it is actually the other way around. DMT can be considered the demethylation product of DET. That is, if you take DET and chop off one methyl from each nitrogen-bound ethyl groups then you get dmt.

I hope this does not throw the theory far out.

In any case, you can argue that Acacias have dmt that gets converted to DET due to the action of not demethylases but methylases. There are plenty of enzymes in the organism that perform transfer of methyl groups. In the latter case, you'll have to somehow demonstrate that DMT is the substrate of some methylase enzyme (it sounds too difficult but not impossible) that can specifically methylate the methyl groups of DMT to form ethyl groups. Even though the later cannot be excluded, itt is borderline science fiction.

 

[benzyme]

In any case, you can argue that Acacias have dmt that gets converted to DET due to the action of not demethylases but methylases.

whoa whoa...let's back up here..

DET is not known to occur in nature.
if it did, the enzyme involved would likely be an ethyl transferase

this is pretty far-fetched. the route from DMT to DET is not very feasible, even in a biological setting.
removing the methyls from DNA is one thing, there are collective forces at work that make the methyl bond seem weak in comparison...but removing the two methyls from the terminal amine on a tryptamine requires a great deal of free energy.in synthesis, the intermediate is susceptible to unwanted side-reactions, including cyclization

 

[Infundibulum]

Yes benzyme, I know DET is definitely not found /has not been found yet in the nature. Ethereal's preposition was that acacia dmt gets converted to det by some sort of "exogenously administered enzyme", which in any case (and from what we already know about enzymes, substrates and enzymatic reactions) seems extremely far-fetched.

Just for the record, ethereal, if for whatever reason you want to look it further you may want to check not DNA methyltransferase (or DNA demethylase enzymes) BUT histone methyltransferases and histone demethylases. The histone proteins that help at the packaging of DNA within eukaryotic cells are continuously methylated, dimethylated, trimethylated as well as progressively demethylated. Most of these methylations occur in the -NH2 group of the basic amino acids lysine and arginine, in a way analogous to the methylation and dimethylation of tryptamine to NMT and DMT.

 

[etherealsamba]

well, in my mind, i would think that the bio essays done on acacias that are published, were probably done mostly on widely available acacias, ie in parts of africa or the middle east that are open to strangers..

but what about those parts that they dont let people just come right in and do what they want?

like, holy places.



as well, Maybe, i had it wrong about the agrocybe theory, maybe there is something in psilocybe or panaelous, enzyme-wise, that would add a methyl group instead of take away, or do the opposite of what i proposed with agrocybe..

also, one might consider:

in fermentation (ie with yeast and enzymes from the air, not using actual yeast cultures) an alcohol derived from vegetation (leaves and barks) would produce methanol, as a fermentation from fruit matter (sugars) would produce ethanol...

maybe a fermentation of acacia leaves, in time would produce those extra methyl groups through methanol production (i dont know how that works) rendering DET..

or DPT, or DiPT etc etc... all active orally without mao inhibition..


i dont know chemistry, but i don't doubt that there is a way to do this.