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DIY Thin Layer Chromatography

Migrated topic.
Admittedly, there's no scale so the blob size might be fine :LOL:
Sounds like your spotting technique is pretty good, then. One more technique is to stretch out the capillary tube in a flame and cut it into two separate micropipettes for even smaller spot size. I would try increasing the concentration of the extract beforehand too, assuming the viscosity remains workable. Of course, at some point overloading of the spot could come into play which causes smearing and poor separation. [Maybe you've already read up on all of this, just putting it down for the record!]
 
Cheers dude

Yeah that's exactly where I'm at to be honest. Working with the highest viscosity possible to ensure proper concentration.
I think my tubes are 0.6mm internal.

I've done alot of soaking of information over the last week that's for sure! Appreciate it bud dude, you can never know too much 👍
 
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