CandianHaze
Rising Star
Hello, I am a new member, I have registered here to get feedback on an extraction experiment that I am conducting, and at the same time hope to join in the community by providing useful dialog and experimentation in the realm of ACRB extraction (And eventually p. cubensis extractions, and san pedro extractions). I'm no poet, nor very spiritual, so my contributions to the community will be of a scientific slant, so with that in mind, my introduction essay will be in the form of an experiment using the scientific method.
Below you will find my method outline, hypothesis etc. of my current experiment.
I would love questions, comments and any general feedback involving my methodology.
The focus of the experiment is on required heat, specifically boils done during the acidic phase of an ACRB extraction.
I am currently in the 3rd Freeze/Warming cycle, and should have a conclusion by the end of this upcoming weekend.
Question: Are acid boils necessary for an ACRB A/B Extraction.
Research: Numerous previous A/B extractions, extensive erowid, dmt-nexus and /r/dmt reading. Anecdotal observations; When doing a A/B of a previous ACRB extraction to improve quality of final product, it was noted that acidic water has very high DMT solubility, 4.3g of non pure (tan, waxy) DMT freebase crystals were completely dissolved in 75ml of PH3 water. This leads me to believe that an extraction can be done without boiling, and that an extensive soak with cold/warm temperature cycles will be enough to extract all the DMT from ACRB.
Hypothesis: Acid boils are not necessary.
Experiment: 200g of ACRB will be extracted using a 'soak and freeze' A/B extraction; the remaining bark will be subjected to an acid boil extraction. Yield from the Boil extraction will be measured.
Method:
Solution Creation.
Soak and Freeze
750ml of PH3 Distilled water will be prepared.
200g of ACRB will be placed in a blender with the 750ml of Water and blended until a fine shredded consistency is attained.
1TBSP of Non-Iodized Kosher Salt is added.
Solution is placed into a ZipLock brand food safe Freezer bag.
Solution is frozen solid, and thawed by placing it into a slow cooker on "Keep Warm" temp range is tested at 46 degrees C with a 1 degree variance.
Solution is kept at this temperature for at least an hour between freeze cycles.
The freeze/warming cycle is repeated a total of 3 times.
Solution is filtered with cheese cloth, and then the solution is again filtered with a fine coffee filter.
500ml of PH3 Water is then added to the separated plant matter, and freeze/warm cycled once more in order to capture any remaining alkaloids possible.
Solution is then filtered with cheesecloth and a coffee filter again. The two batches of solution are combined.
1 TSP of Zinc powder is then added to the solution.
The solution will be left in a dark area to naturally evaporate down to 750ml.
The solution is then filtered through a coffee filter twice to filter out the zinc powder.
Boil
750ml of PH3 Distilled water will be prepared.
The remaining plant material from the Soak and Freeze method will be combined with the PH3 Water
The solution will be subjected to a low boil for no less than one hour.
The solution will be closely monitored and water will be added to maintain the volume of liquid.
The solution will be filtered with cheesecloth and coffee filters in the same method as the soak and freeze prep.
Another 500ml of PH3 water will added and the same method of boiling and maintaining water level will be applied.
The plant matter will once again be filtered via cheesecloth and coffee filters, the resulting solutions will be combined and reduced to 850ml via slow boil
1 TSP of Zinc powder is then added to the solution.
The solution will be left in a dark area to naturally evaporate down to 750ml, this is mainly to allow the zinc to do its work.
The solution is then Filtered through a coffee filter twice to filter out the zinc powder.
Extraction
Both solutions will be subjected to 100ml of naphtha to remove excess fats and oils, the naphtha will be decanted, labeled and set aside until the method has been completed to insure no loss of final product.
The defat step will be repeated on both solutions a second time.
Both solutions will have their PH raised via Lye to a minimum of 13.
100ml of Light naphtha will be used to extract the alkaloids
Naphtha will be decanted and freeze precipitation used.
Naphtha will be recycled and reused on their respective solutions until crystals no longer form during freeze precipitation.
The remaining naphtha used in the extraction step will be evaporated
Final yields will be measured.
Materials used:
200g ACRB
Distilled Water
Kosher Salt
Zinc Powder
600ml Light Naphtha.
Fumaric Acid
Lye
Tools Used:
Glass crystalization trays with PTFE airlight lids.
Glass pipette
1 Litre Mason Jars
Stainless Steel Cookware
Ziplock brand foodsafe freezer bags(Large)
Digital Scale
Digital PH Tester
Expectations: The yield from the acid boil will be nearly non existent.
Conclusions: Unknown
Below you will find my method outline, hypothesis etc. of my current experiment.
I would love questions, comments and any general feedback involving my methodology.
The focus of the experiment is on required heat, specifically boils done during the acidic phase of an ACRB extraction.
I am currently in the 3rd Freeze/Warming cycle, and should have a conclusion by the end of this upcoming weekend.
Question: Are acid boils necessary for an ACRB A/B Extraction.
Research: Numerous previous A/B extractions, extensive erowid, dmt-nexus and /r/dmt reading. Anecdotal observations; When doing a A/B of a previous ACRB extraction to improve quality of final product, it was noted that acidic water has very high DMT solubility, 4.3g of non pure (tan, waxy) DMT freebase crystals were completely dissolved in 75ml of PH3 water. This leads me to believe that an extraction can be done without boiling, and that an extensive soak with cold/warm temperature cycles will be enough to extract all the DMT from ACRB.
Hypothesis: Acid boils are not necessary.
Experiment: 200g of ACRB will be extracted using a 'soak and freeze' A/B extraction; the remaining bark will be subjected to an acid boil extraction. Yield from the Boil extraction will be measured.
Method:
Solution Creation.
Soak and Freeze
750ml of PH3 Distilled water will be prepared.
200g of ACRB will be placed in a blender with the 750ml of Water and blended until a fine shredded consistency is attained.
1TBSP of Non-Iodized Kosher Salt is added.
Solution is placed into a ZipLock brand food safe Freezer bag.
Solution is frozen solid, and thawed by placing it into a slow cooker on "Keep Warm" temp range is tested at 46 degrees C with a 1 degree variance.
Solution is kept at this temperature for at least an hour between freeze cycles.
The freeze/warming cycle is repeated a total of 3 times.
Solution is filtered with cheese cloth, and then the solution is again filtered with a fine coffee filter.
500ml of PH3 Water is then added to the separated plant matter, and freeze/warm cycled once more in order to capture any remaining alkaloids possible.
Solution is then filtered with cheesecloth and a coffee filter again. The two batches of solution are combined.
1 TSP of Zinc powder is then added to the solution.
The solution will be left in a dark area to naturally evaporate down to 750ml.
The solution is then filtered through a coffee filter twice to filter out the zinc powder.
Boil
750ml of PH3 Distilled water will be prepared.
The remaining plant material from the Soak and Freeze method will be combined with the PH3 Water
The solution will be subjected to a low boil for no less than one hour.
The solution will be closely monitored and water will be added to maintain the volume of liquid.
The solution will be filtered with cheesecloth and coffee filters in the same method as the soak and freeze prep.
Another 500ml of PH3 water will added and the same method of boiling and maintaining water level will be applied.
The plant matter will once again be filtered via cheesecloth and coffee filters, the resulting solutions will be combined and reduced to 850ml via slow boil
1 TSP of Zinc powder is then added to the solution.
The solution will be left in a dark area to naturally evaporate down to 750ml, this is mainly to allow the zinc to do its work.
The solution is then Filtered through a coffee filter twice to filter out the zinc powder.
Extraction
Both solutions will be subjected to 100ml of naphtha to remove excess fats and oils, the naphtha will be decanted, labeled and set aside until the method has been completed to insure no loss of final product.
The defat step will be repeated on both solutions a second time.
Both solutions will have their PH raised via Lye to a minimum of 13.
100ml of Light naphtha will be used to extract the alkaloids
Naphtha will be decanted and freeze precipitation used.
Naphtha will be recycled and reused on their respective solutions until crystals no longer form during freeze precipitation.
The remaining naphtha used in the extraction step will be evaporated
Final yields will be measured.
Materials used:
200g ACRB
Distilled Water
Kosher Salt
Zinc Powder
600ml Light Naphtha.
Fumaric Acid
Lye
Tools Used:
Glass crystalization trays with PTFE airlight lids.
Glass pipette
1 Litre Mason Jars
Stainless Steel Cookware
Ziplock brand foodsafe freezer bags(Large)
Digital Scale
Digital PH Tester
Expectations: The yield from the acid boil will be nearly non existent.
Conclusions: Unknown
