benzyme said:VisualDistortion said:Why would it need to be bitartrate? I thought ascorbic acid and tartic acid where very similar anyways.
qualitatively, they are
the problem is, dcm can interact to some degree with polar molecules, despite not having any proton-donors or acceptors.
it's odd like that
benzyme said:D-tartaric, specifically (the d-isomer helps preserve the stereochemistry of the intermediate).
chloroform is a harder to come by, as it's used little outside of research; it's been widely replaced by the less toxic methylene chloride, although both are obtainable if one searches thoroughly.
are you sure it was chloroform? I was under the impression that chlorine ions isomerize lsd :?
TEK:
Elves indicated using 28 grams dried mixed cubensis chopped and placed into jar.
Began by soaking in pure grain (everclear) 190 proof alcohol. Finely chopped mushrooms in jar.
Then the jar sat in warm water bath for 7 hours. This was done by placing a rag in the bottom of a pan and filling it up with some water.
Placing the jar of soaking fungi on the rag in the water and bringing the heat up slowly.
* NOTE: Alcohol boils at a lower temp. than water. Watch it closely. you don't want the alcohol to boil!
Straining and squeezing the fungal matter 3 times. Saving the broth each time. Filtering many times while hot. Through coffee filters.
The combined strainings were then added back to the heat bath and brought up to almost a boil.
Finally reduced a bit then began evaping.
The elves told me they wanted to pour off the darker pure grain and wash the crystals in some fresh everclear while they are frozen.
Put into final resting place and add just enough fresh everclear to keep the crystals covered to store in the freezer.
The elves said that was a dime in the background and that they harvested approximately 4 times that amount from the 28 grams of fungus.
They (elves) impress me so.
For matters pressing the elves induced me into what they described as a 30mg vaporized crystal experience. It came on about 1 minute 30 seconds into the induction. The initial rush was more profound than vaporized DMT experiences but not as intense as 5meodmt vaporized. The visual aspect was that of DMT and maybe even a bit more, hard to say, there was much going on.
Auditory and physical symptoms persisted throughout the 20 minute peaking experience. The come down was much like the downside of oral administration or fungi. After effects persisted for about 30 - 40 minutes after peak.
All in all the elves have stumbled upon something wonderful and overall overwhleming.
Cheers.
Ask Dr. Shulgin Online
ARCHIVE: March 5, 2003
Psilocybe Mushroom Extractions
Source.
VisualDistortion said:benzyme said:D-tartaric, specifically (the d-isomer helps preserve the stereochemistry of the intermediate).
chloroform is a harder to come by, as it's used little outside of research; it's been widely replaced by the less toxic methylene chloride, although both are obtainable if one searches thoroughly.
are you sure it was chloroform? I was under the impression that chlorine ions isomerize lsd :?
You know what, it is because it was not in chloroform. Been a long time since I last looked at Otto Snow's work. He had LSD in methanol, and precipitated it by adding a solution of tartic acid/MeOH. Lol, silly me.
Maybe put the fungus in a sohxlet and extract with MeOH, and then add a solution of tartric acid/MeOH to precipitate the goodies?
I am just trying to take the principals of one method and apply it to another situation. I don't understand this stuff like you do, but I am still hopeful I can figure out something good.
Endlessness and nen888 also found a (200) peak years ago when working on acacia extracts. Looks like they determined it was leptocladine. Endlessness pointed out several theoretical secondary peaks with (157) among them.Loveall said:The largest peak at 202 (200) is unkown at this time. We are not sure of what it is, psilocin should have been at 206 (204), and a simple oxidized form of psilocin at 204 (202). Would be grateful if anyone had any theories on what this peak is.
Tengukashi said:So this is a brilliant thing to know, and I'm really excited to test if heptane works as well as theorized. However, I remember in other extraction threads that there needs to be a certain pH for this to work. I also remember benzyme talking about a psilo extraction that involved diethyl ether, though I can't for the life remember what that invovled or where I saw it. Maybe in chat?
But back to the issue at hand: does this freeze precip need to be at a certain pH at each stage in order for it to work?
Edit: I also found an instructable for a simple anaerobic chamber, since psilocin need an oxygen free space. This can easily be applied to other oxygen-sensitive compounds, and most of these boxes are made with polypropylene, which resists most chemicals pretty well: DIY Anaerobic Chamber (aka Glove Box)
Orion said:I just want to thank you for the contributions in these many threads Loveall. Some of these results seem quite promising. I'm fairly certain of the blue and blue green colours being a good indicator of the presence and degradation of psilocin.
I am lost as to why no psilocin was found in the analysis, even though the xylene clearly shows a pale greyish blue discoloration. Seems to me that psilocin did move into the xylene. Was the psilocin fumarate somehow converted to pure psilocin before analysis or did it remain a salt? Could either one of these degrade into an unknown form before being analysed?
One other thing. You mentioned other actives were present in the extract, is it reasonable to assume these could be the cause of the perceived activity if not placebo ? This could mean that all of the psilocin was still in the xylene but FASA could still produce an active extract free of psilocin (...IF that's the case).
I'm also interested in the idea of not having to dephosphorylate the psilocybin before having migrate into a separate nonpolar phase. This bypasses a lot of problems if possible.