Ipomoea- A/B -> FASI

[kerelsk]

Hi I recently made an Ipomoea tricolor extraction as such:

1. Pulverized & ground the seeds to a powder, added cool water that was previosly boiled to remove chlorine. Excess NaOH added with sufficient water to make a relatively thick soup.

2. Extract alks with limonene
3. Extract alk salts with vinegar
4. Evaporate vinegar to smaller volume, add Na2CO3 till no reaction. Dry completely to crust.
5. Pull freebase alk crust with iPrOH
6. Add FASI to iPrOH to precip alk salts
Now I'm left with some pretty xtals it looks like, suspended delicately in alcohol

Any ideas how I can isolate these babies? Feel like filtering with a coffee filter or cotton wouldn't suffice

Can I blot out the alcohol with paper maybe?

 

[Ruffles]

Very interesting.

What was the "reaction" you got on step 4? Can you try a UV light over the white stuff you got to see if you got some fluor in it? Any bioassay done on it?

Why not keep the iso plus magic in a freezer?

 

[kerelsk]

Step 4 is turning Ipomoea alkaloid acetates into freebase and dehydrating the extract before then pulling with relatively anhydrous isopropanol.

I hypothesized addition or FASI would crash out the LSA, which is what seemed to have happened.

As precipitated crystals they don't appear to fluoresce well.


I think the best idea would be to get a centrifuge so I can quickly shake fresh solvent in, centrifuge, and decant. So I guess I should buy one ...

Once I get it out of the container and try it I'll update

 

[Ruffles]

Got it on step 4. So what does the dry stuff look like after drying? Gooey brown stuff? How did you dry it, wind, heat? Then what is left over after ipa addition? And the loaded ipa looks like?

Perhaps testing uv light at this alkaline ipa stage instead of the salt? If it's blue or green then and after salting it loses the fluor?

Your tek looks very very interesting, we might give it a shot.

 

[kerelsk]

Oh also to clarify on step 4- I add sodium carb until bubbling stops and then I add a bit more. Excess essentially but not a huge amount.

Dried in a food dehydrator on high. Once dried it looks like white sodium acetate crystals with a yellow impurity. Minimal isopropyl alcohol will pick it up from the sodium acetate/sodium carb matrix.

The loaded IPA has just a slight yellow tinge. It doesn't grab all the yellow pigments seemingly, or very well.

Yes slight fluorescence before xtal, not after.