So today I finally got the time to start the in vitro test.
MS solution with 2g/l gelrite and 30g/l sugar with additional rooting substances.
I cut off one Turkey-Red. It gave 6 nodes.
I planted them in different positions, to see if this makes a difference.
(node 1cm above surface, node just above surface, node slightly under the surface, flat, flat submerged)
The tops I just put into a solution standing. Maybe it works, maybe not...
Attached a pic:
Edit 12.7.2017, short update:
Doesn't look good. Saw yesterday some mold on some of them. So bought a fungicide and sprayed every glass.
Not a single one shows any sign of making roots yet.
Edit 13.7.2017, short update:
Still no sign of roots. But they started to grow, which some did faster than I thought of. So in one glass it already reached the lid and one of the flat ones is already longer than the glass is wide.
Probably next time I should rather put them in test tubes which are higher.
Edit 14.7.2017:
Doesn't look too good. Mold came back, and big, although I sprayed them with fungicide 2 days ago. Sprayed again. One seems to have already died due to fungus.
If I ever do another run, I will probably try to mix in some fungicide directly in the solution.
Edit 16.7.2017:
My heart really bleeds. They are really growing very good (no comparison with my experiments in soil), but still no signs of rooting.
But the mold is starting to completely take over. Even spraying them daily with fungicide is futile. It seems the mold grew into the solution and there the fungicide cannot reach.
Next time I really have to try adding fungicide into the base solution, although I have no idea how this will affect the plants.
Edit 1.8.2017:
They all died now...
Except for the one who immediately died in the beginning and the horizontal ones, all did grow to a size of about 25-30cm (from an initial 5cm cutting) and then started to die off.
None showed even the slightest hint of making roots
I did make one new glass with added fungicide. The one I used previously + a new one. But that didn't help. Also there the mold immediately came.
I'm really wondering why these normal fungicides from the garden story seem to have (almost) no effect on mold.
Probably they are for other types of fungi?
But then what should I take against mold fungi?