Research The Acacia Analysis Thread

[61pete]

oh right i forgot about that. i literally posted to that thread.
so. is that pretty reliable/consistent, that it will fluoresce if theres *any* DMT in the bark?
Since i dont have a known sample to test i dont know exactly how bright it should glow, like, at point blank would i see it in daytime, or is it a dull glow you can see in a dark room?
Sadly i tried shining the light on my accuminata bark powder, but i could not observe anything at all, other than a small amount of lighter material that was obviously just light colored wood, i know its not the same but, still disappointed my actual sample didnt work. I have also tested this on p. aquatica in the past, no effect either.
but, i guess either way theres nothing left for me to do but test some nearby trees with a file and my UV light

You'll only see it in the dark/ at night.
Im kinda new to this UV thing and only have used the light on 1 species, so I cant say for sure, but the general feeling is that DMT should available when the bark glows under UV.
I see something crashing out of the nps in the freezer, but its only been 2 hrs, so I'll know better in the morning.


Theres not much blue on my test branch, but a lot of dark purple streaks.
In daylight this is all a sand/tan color.




A day later, nothing precipitated out of the nps that survived fan drying, so possibly some ice clumps.
perhaps blue is the more important color than dark purple.

 

[drpotato]

You'll only see it in the dark/ at night.
Im kinda new to this UV thing and only have used the light on 1 species, so I cant say for sure, but the general feeling is that DMT should available when the bark glows under UV.
I see something crashing out of the nps in the freezer, but its only been 2 hrs, so I'll know better in the morning.


Theres not much blue on my test branch, but a lot of dark purple streaks.
In daylight this is all a sand/tan color.

View attachment 102739

this is very helpful thankyou

 

[acacian]

this is something i have been wondering about, is it the case that DMT is higher during flowering? or just before/after?
I havent seen someone explicitly mention this, for vegetables at least flowering is not a great time for harvest, but this is a tree of course haha.

Fortunately at least, DMT is always there, just not as much or consistently all year round if you can ID the thing without the flowers.


Thanks to cyclone alfred theres a lot of acacias around here now which have snapped branches, so soon ill be collecting some samples to run colormetric testing on, and i get to benefit from this short term access to sustainable wood-bark.

Another question i have: for something like maidenii, or leiocalyx, or any others that are very similar in appearance and found in the same places, lets call them maidenii-like, low DMT content, and lots of fat, wax and resin, like acuminata on the opposite of steroids, what would be a good strategy for field-testing for whether a given tree is worth collecting from? ideally you dont want to spend time and resources processing empty leaf/bark but, you are taking from the tree too, if you can avoid a weaker / non active or lookalike that would be great. Has anyone developed a shortcut method to apply say, marquis reagent out in the field? i assume some degree of extraction is required before any DMT is concentrated and clean enough for the test to produce useful results.
Best i could think of was burning a small piece of bark or leaf in a test tube under hard vacuum since hand pumps can get small volumes easily down to about -700mmHg in my experience, and then testing that assuming a smell test at that point wasnt sufficient.
I accidentally distilled the DMT out of the collective toluene pulls from ACRB because the oil was so clear i didnt notice it getting thick and thought i could distill off more solvent before doing FASA. turned out i had a LOT of fats and other gunk there, or they held onto toluene really well, either way, it started smoking and later i collected 30mg of dmt from the toluene distillate. since i used a shortpath, i think i lost a lot as smoke in the time i had my back turned, the worst kind of condenser for entraining smoke.

Hey @drpotato ,

Many swear by the burn test and the taste test. Personally I don't find them super reliable... but each to their own. Maybe smoking has ruined my sense of taste and smell. A lot of Acacia is very bitter and to me the smoke smells tryptamine like too often. That said, bitter usually will at least produce an extract.. it just might not be DMT. Closest thing to an 'in-field' test I can think of is collect a few grams of material, shake in a vial for a few minutes, in ethanol/methanol, reduce and put on paper for reagent or TLC. Reagent is only an indicator of course, but still useful in combination with other indicators.

I also won't test a plant unless it really gives off a special vibe to me. That's obviously very personal but has always been my signal to test..

 

[drpotato]

What exactly is the burn test? ive heard it a lot but, never described. theres quite a few ways to burn something after all. is it just haphazardly getting some leaf or bark to smoulder?

TLC seems like it would be pretty reliable however, it might be worthwhile to just get good at some sort of high speed variation and if need be i can always bring along a refference sample which i think should work to compare two rising samples, a void next to the known DMT would pretty quickly tell me its no good. I did also just find some deep UV LEDs, so assuming those dont work for identifying bark with a DMT layer, 275nm or so is still enough to light up a DMT band on TLC i think.

if you can reagent test the dmt band on TLC though that would be pretty handy