Research Haphazard Oral Administration Thread (Salvine Tek)

[stuartroelke]

Had this idea for a while now, but I'm testing a "full-spectrum" extraction with 95% ethanol AND phosphatidylcholine (first attachment shows PC dissolving in alcohol). I won't explain the entire process at the moment—it could end up being in the Salvine "Pharma" tek.

Look at that emerald green! (second attachment)
Compared to prior "full-spectrum" extracts, the chlorophyl isn't oxidizing olive / brown like it usually does (see previous extraction).
Oxidized leaf powder still produced effects when used in the crude tek—who knows if the rich green actually means anything good other than PC slowing magnesium displacement. Still gotta look into the effects of air exposure on salvinorin A.

I also ordered bile, as effects for all pharma tests have been significantly stronger when consumed right before—or after—a large meal. It's possible that the salvia "matcha" used in my crude tek has been predictable BECAUSE the bitter taste triggers bile release. Bile salts would have an additive effect when combined with PC, and my sorta successful tests with EVOO likely demonstrate how triggering bile release with monounsaturated fats will cause a low-level experience. Perhaps consuming tiny capsules prevent enough bile production from occurring?

Unfortunately—if ox bile supplements do work—then I'll likely end up adding bile salts to my evaporation tray I'll also need to find a low-volume vegan alternative. Taurocholic acid is unfortunately derived from cattle bile. Synthesized TUDCA? Any thoughts?

 

[stuartroelke]

Extracting with 95% ethanol and PC is rough—it's like oil paint (first and second images). I do not have the patience to dry it. I'll use microcrystalline cellulose or silicon dioxide in the future when necessary. Managed to get one "threshold" dose into a capsule (third image—equivalent of ~4g S. divinorum leaf). I subtracted the weight of the plastic wrap and capsule; don't worry.

This is my current logic tree for how to proceed with the next four doses, and it's based off a strong experience I had after taking half my previous "full-spectrum" pharma dose (~2g leaf, 50% water + 50% solvent extraction, 250mg PC) when taken 30 minutes after consuming 1tsp turmeric (supposedly inhibits CES1 and PLA2) and right before eating a fatty meal (bile salt mimetic):

1. Take one capsule far away from any meal and without CES1 or PLA2 inhibitors. If this doesn't work, then either bile salts, CES1 inhibition, and/or PLA2 inhibition are essential (S. divinorum leaf powder may naturally contain CES1 and PLA2 inhibitors). Took the capsule today 3-4 hours after a meal and felt nothing—not even placebo. Consumed food and yerba mate and hour after and still had no noticeable effects.

2. Take one capsule 30 minutes after consuming 1tsp turmeric. If that works, then it definitely seems to be related to CES1 / PLA2 inhibition or bile release caused by the bitter taste. If it doesn't work, then still proceed.

3. Take one capsule with 500mg ox bile and no CES1 or PLA2 inhibitors. If that works, then bile salts are the key. If this didn't work, but the turmeric did work, then return to the previous full-spectrum extract (50% water + 50% solvent—easier to process) and combine with PC and ox bile. If neither worked, then proceed.

4. Take the final dose with 4g spent salvia powder (already extracted) and no meals, ox bile, inhibitors, etc. If this works, then reassess the extraction process. If it doesn't, then proceed.

5. Take 4g of spent salvia powder (salvinorin A removed) with PC, and without extract, meals, ox bile, inhibitors, etc. If this works, then I might have to take a long break from this research because that wouldn't make any sense to me. Phospholipids increasing the absorption of some random hydrophilic compound would seem insane.

Final random thought before I forget:

Could tannins be contributing in some meaningful way? Are they inhibitors? Do they meaningfully saturate esterases? What's a reasonable tannin to take as a supplement if future tests become necessary? Or, could quercetin be part of the bitter taste in S. divinorum leaves that potentiates the crude ROA? Does betulinic acid inhibit both CES1 and PLA2, and does it make sense to extract that myself instead of buying quercetin supplements? Just read that Omega-3 fatty acids are PLA2 inhibitors—could fish oil potentiate Salvine?

And, are there actually any vegan alternatives to ox bile?!

 

[stuartroelke]

I don't usually do two tests back-to-back—as I'm afraid tolerance could mess with results—but I wanted to take advantage of days I have off due to the severe weather. I'm also less concerned about tolerance because yesterday didn't involve any perceptible salviaic sensations.

2. Take one capsule 30 minutes after consuming 1tsp turmeric. If that works, then it definitely seems to be related to CES1 / PLA2 inhibition or bile release caused by the bitter taste. If it doesn't work, then still proceed. Ended up ingesting 2tsp turmeric 3 hours after a meal because research led me to believe it wouldn't be enough CES1 / PLA2 inhibition anyway. Took the capsule thirty minutes later. Eventually felt the tiniest perceptible salvia buzz for an hour. Consumed a handful of chocolate and food, then felt it slightly more. A "nearly placebo" experience.

This test—and clues from previous attempts—makes me believe bile is essential. Ingesting a threshold dose using the crude method (4g of S. divinorum powder "matcha" and 2g of lecithin) likely causes enough bile salt release to provide a necessary cumulative effect; that just isn't happening with liposome capsules. I've already got an Ox Bile supplement on order to test.

 

[stuartroelke]

3. Take one capsule with 500mg ox bile and no CES1 or PLA2 inhibitors. If that works, then bile salts are the key. If this didn't work, but the turmeric did work, then return to the previous full-spectrum extract (50% water + 50% solvent—easier to process) and combine with PC and ox bile. If neither worked, then proceed. This did not work well enough to consider it a success. I'm actually going to return to my full-spectrum extract regardless. Either Salvia divinorum contains potent and hydrophilic CES1 / PLA2 inhibitors—perhaps compounds that haven't been documented yet—or, extracting with solvent and PC preemptively encapsulated the wrong lipophilic compounds.

I'm still going to recommend folks consume a small snack or meal before taking crude Salvine; some people are struggling to achieve a decent experience without food. As for myself and a few others, the current tek works consistently and reliably with only lecithin and Salvia divinorum powder. Next test is to combine full-spectrum soft extract with ox bile and PC to determine whether or not bile salts are contributing anything to the experience.

Hopefully that combination will fit into the capsules I have.
Otherwise, I'll be swallowing a small ball of lipid matrix

 

[stuartroelke]

I took a capsule of the discard Salvia divinorum powder with—from my PC + solvent extraction—with 500mg PC to see if I would feel anything. I did not.

I may try taking more on Wednesday just to be absolutely sure that all of the salvinorin A was successfully pulled out of it. Primarily doing this to prove that the extraction was not a fluke, and that that full-spectrum extract works better than any single component will on its own.