albertfive123
Esteemed member
look u know what i think soli and climat test are great idea. i am gardener for a living and i wanna know more. can u tell me specific what soli and climate surcomestances u want to try ?
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Research The nexian phalaris breeding programme
- Thread starter Grasshoppers
- Start date
Research done by (or for) the DMT-Nexus community
<Ahem>
I'd say those guys are already pretty serious about this
Grasshoppers
Titanium Teammate
Enhancements in Thin-Layer Chromatography (TLC) Protocols
This season’s workflow saw several key upgrades to our TLC testing, focusing on sample prep efficiency and high-precision digital analysis. We shifted toward a more robust extraction method and integrated custom hardware for both sample loading and data acquisition.
1. Sample Preparation & Extraction
To ensure a high concentration of target analytes, we refined the extraction ratio and alkaline environment.- Protocol: 25 mg of desiccated leaf tissue is submerged in 1.0 mL of Methyl tert-butyl ether (MTBE).
- Alkalization: 3 drops of aqueous ammonia are added to facilitate the release of free-base alkaloids.
- Duration: Following a period of vigorous mechanical agitation, the samples are left to macerate for 8 hours to reach extraction equilibrium.
2. Precision Spotting
We moved away from manual capillary spotting to achieve better resolution and reproducibility.- Hardware: A custom piezoelectric TLC loader was developed, utilizing a modified piezo buzzer as the micro-dispensing head.
- Result: This allows for the application of narrow, uniform "lines" rather than erratic spots, significantly reducing band broadening during development.
3. Chromatography Parameters
We opted for a straightforward, high-polarity mobile phase to suit our specific chemical targets.- Stationary Phase: Unmodified 60Å Silica Gel (non-fluorescent) TLC plates.
- Mobile Phase: Isopropanol (IPA) and 25% aqueous ammonia in a 12:1 ratio.
- Method: Ascending development in a saturated chamber.
4. Digital Imaging & Fluorescence Induction
The most significant leap was in our detection and quantification setup. By bypassing standard consumer cameras, we gained much better raw data control.- Sensor Logic: Plates are imaged using an IMX462 back-illuminated sensor. This sensor is particularly effective due to its high near-infrared (NIR) sensitivity and low noise floor.
- Processing: Raw data is pulled directly from the Bayer array via MIPI-CSI onto a Raspberry Pi, allowing us to process the signal without aggressive compression artifacts.
- Excitation: Dual-wavelength UV LEDs are used to induce fluorescence:
- 275 nm: For short-wave excitation of primary aromatics.
- 365 nm: For long-wave fluorescence of conjugated systems.
Exemplary Plate Analysis:
wet plate exposed to 275nm
dry plate exposed to 275nm
Grasshoppers
Titanium Teammate
How to Interpret a Rich Phalaris aquatica TLC Profile
The image shows an example TLC lane from a particularly rich sample within the Phalaris aquatica breeding line, annotated with the identified compounds at their positions.
Methoxylated and non-methoxylated compounds typically occur in pairs, with the methoxylated compound showing a slightly lower Rf value than its non-methoxylated counterpart.
As visible in the image, interpretation of this example lane is relatively straightforward.
Do you have a link to some more details on this? My searches so far have turned up nothing of use.
Grasshoppers
Titanium Teammate
I’m using a modified 27 mm piezo element from AliExpress (20 pcs for around $1).
To adapt it, I ground away the lead zirconate titanate material in the center, created a small hole in the metal base plate using a tungsten carbide needle, and epoxy-glued a small funnel above the opening holding the sample fluid.
The piezo is driven with short pulses to dispense individual droplets.
For positioning, I use a NEMA 11 linear drive to move the piezo above the TLC plate while pulsing. Droplets are deposited with a spacing of approximately 200 µm.
To adapt it, I ground away the lead zirconate titanate material in the center, created a small hole in the metal base plate using a tungsten carbide needle, and epoxy-glued a small funnel above the opening holding the sample fluid.
The piezo is driven with short pulses to dispense individual droplets.
For positioning, I use a NEMA 11 linear drive to move the piezo above the TLC plate while pulsing. Droplets are deposited with a spacing of approximately 200 µm.
Fabulous, thank you very much indeed! This alone deserves highlighting in its own thread somewhere nearby
Grasshoppers
Titanium Teammate
Breeding for DMT Produces Three Distinct Chemical Types in Phalaris aquatica
Following selection and selective breeding of Phalaris aquatica for DMT and related tryptamines, three distinct chemical profiles have emerged within the breeding line. These chemotypes appear to be genetically determined; individual plants do not switch between them. However, the relative proportions of individual alkaloids can vary somewhat depending on environmental factors such as climate and season.
Type 1: DMT-dominant
Type 2: DMT and 5-MeO-DMT
Type 3: 5-MeO-DMT and 5-MeO-NMT
The precise psychedelic properties associated with these different chemotypes are currently under investigation.
Interestingly, gramine-producing plants, as well as plants with generally low tryptamine content, disappeared from the breeding population after just a single generation of selection for elevated levels of DMT and related compounds.
Following selection and selective breeding of Phalaris aquatica for DMT and related tryptamines, three distinct chemical profiles have emerged within the breeding line. These chemotypes appear to be genetically determined; individual plants do not switch between them. However, the relative proportions of individual alkaloids can vary somewhat depending on environmental factors such as climate and season.
Type 1: DMT-dominant
Type 2: DMT and 5-MeO-DMT
Type 3: 5-MeO-DMT and 5-MeO-NMT
The precise psychedelic properties associated with these different chemotypes are currently under investigation.
Interestingly, gramine-producing plants, as well as plants with generally low tryptamine content, disappeared from the breeding population after just a single generation of selection for elevated levels of DMT and related compounds.
Incredible work. I haven't been here since 2014, and at that point we were just seeing people start working with Phalaris, with many warning against gramine. I just read the entire thread and while I only understand half of it, it's amazing that the research is being done -- and by such serious and capable people. I just had to stop in and say this.
Very excited to see where this leads. The very idea of having plants specifically bred for this purpose is so so cool.
Very excited to see where this leads. The very idea of having plants specifically bred for this purpose is so so cool.
neurobloom
Titanium Teammate
Major Milestone: Base Breedline Seeds from the Breeding Programme Now Available for release
Dear Phalaris enthusiasts
Today marks a significant milestone in our breeding programme. After years of systematic selection, chemical profiling, and careful cultivation, we are proud to announce that the first seeds from our base breedline are now available for release.
This is the foundation upon which our future DMT and 5-MeO-DMT cultivars are currently being built—and we're making it available to the community.
---
The Breedline: Three Distinct Chemical Types
The seeds now available for release represent a base breedline population.
This is not yet a stabilized cultivar, but rather the foundational population from which our future cultivars will emerge.
This breedline contains three distinct chemical types:
Research - The nexian phalaris breeding programme
Type 1 – DMT-Dominant: High DMT content this is the cleanest chemotype within the breedline population.
Type 2 – 5-MeO-DMT-Dominant: High 5-MeO-DMT content, no DMT (often contains a small ratio of 5-meo-nmt)
Type 3 – Balanced/Mixed: Contains both DMT and 5-MeO-DMT in varying ratios (no 5-meo-nmt) if you're into mixing DMT with 5-meo-dmt this is the only botanical source that reliably contains both. (a favorite of mine)
All three types have been preselected for high yield—there are no low-yielding genetics in this population. If you grow and extract from this seed population, you'll obtain a mix of all three types.
If you have access to TLC (or can send single-clone samples for analysis), you can select specific DMT or 5-MeO-DMT clones with very low to non-existent gramine content. The entire breedline population is already notably low in gramine (strong selection pressure was applied to remove Gramine), with only a minority of outlier clones showing high concentrations.
---
Release Information
-What's Available: Base breedline seeds (mixed population containing all three chemical types)
- Quantity: Limited initial release (6.1g which equates to roughly 6100 seeds) First come, first served
- How to order: DM me
---
#
Important Disclaimers
1. For Research Purposes Only: These seeds are being distributed exclusively for research, educational purposes
2. Chemotype Variability: This is a base breedline, not a stabilized cultivar. Individual plants will vary in their chemical profiles. TLC analysis is essential for identifying and selecting specific chemotypes.
---
Looking Forward
This seed release is just the beginning. We envision:
- Community-driven selection: Researchers growing and selecting their own preferred chemotypes.
- Future cultivar releases: Stabilized DMT and 5-MeO-DMT cultivars from this base population
- Expanded collaboration: More data, more analysis, and deeper understanding of this remarkable species
---
Acknowledgments
This milestone would not have been possible without the collective effort, data sharing, and constructive dialogue of this community. Thank you to everyone who has contributed observations, TLC analyses, and thoughtful discussion over the years.
---
To place an order or inquire further, drop a DM and I'll try to respond as soon as I can.
Seeds of segregated DMT and 5-meo-dmt breedlines will be available Next spring! We look forward to seeing where this next chapter of our research takes us.
Major Milestone: Base Breedline Seeds from the Breeding Programme Now Available for release Dear Phalaris enthusiasts
Today marks a significant milestone in our breeding programme. After years of systematic selection, chemical profiling, and careful cultivation, we are proud to announce that the first seeds from our base breedline are now available for release.
This is the foundation upon which our future DMT and 5-MeO-DMT cultivars are currently being built—and we're making it available to the community.
---
The Breedline: Three Distinct Chemical TypesThe seeds now available for release represent a base breedline population.
This is not yet a stabilized cultivar, but rather the foundational population from which our future cultivars will emerge.
This breedline contains three distinct chemical types:
Research - The nexian phalaris breeding programme
Type 1 – DMT-Dominant: High DMT content this is the cleanest chemotype within the breedline population.
Type 2 – 5-MeO-DMT-Dominant: High 5-MeO-DMT content, no DMT (often contains a small ratio of 5-meo-nmt)
Type 3 – Balanced/Mixed: Contains both DMT and 5-MeO-DMT in varying ratios (no 5-meo-nmt) if you're into mixing DMT with 5-meo-dmt this is the only botanical source that reliably contains both. (a favorite of mine)
All three types have been preselected for high yield—there are no low-yielding genetics in this population. If you grow and extract from this seed population, you'll obtain a mix of all three types.
If you have access to TLC (or can send single-clone samples for analysis), you can select specific DMT or 5-MeO-DMT clones with very low to non-existent gramine content. The entire breedline population is already notably low in gramine (strong selection pressure was applied to remove Gramine), with only a minority of outlier clones showing high concentrations.
---
Release Information-What's Available: Base breedline seeds (mixed population containing all three chemical types)
- Quantity: Limited initial release (6.1g which equates to roughly 6100 seeds) First come, first served
- How to order: DM me
---
#
Important Disclaimers1. For Research Purposes Only: These seeds are being distributed exclusively for research, educational purposes
2. Chemotype Variability: This is a base breedline, not a stabilized cultivar. Individual plants will vary in their chemical profiles. TLC analysis is essential for identifying and selecting specific chemotypes.
---
Looking ForwardThis seed release is just the beginning. We envision:
- Community-driven selection: Researchers growing and selecting their own preferred chemotypes.
- Future cultivar releases: Stabilized DMT and 5-MeO-DMT cultivars from this base population
- Expanded collaboration: More data, more analysis, and deeper understanding of this remarkable species
---
AcknowledgmentsThis milestone would not have been possible without the collective effort, data sharing, and constructive dialogue of this community. Thank you to everyone who has contributed observations, TLC analyses, and thoughtful discussion over the years.
---
To place an order or inquire further, drop a DM and I'll try to respond as soon as I can.
Seeds of segregated DMT and 5-meo-dmt breedlines will be available Next spring! We look forward to seeing where this next chapter of our research takes us.
Attachments
Ivan Illich
Established member
Stunning work! I had to make an account just to reply. I studied plant sciences and biotechnology, and I am absolutely in awe with the work being done here. I would love to contribute!
I had a few questions regarding with the breeding:
- what kind of characteristics are being tracked and do you have a pedigree available showing the lineage of your breeding process?
- What does your breeding process look like? What methodology do you apply here e.g what are the "controlled breeding procedures" in the opening of this thread?
- How do you clone your plants?
- Have you made tissue cultures?
I am reading here that Philaris is tetraploid (4n chromosomes), and that it is self incompatible. This makes it significantly more challenging to get a stable "True to seed". "True to seed" normally means homozygosity, which would be achieved with selfing. I am guessing here you are using recurrent selection to breed at a population level?
No one seems to have developed a method to create haploids, which could be really useful in the breeding process.
I would love to get some seeds and to get in touch in the future! I am currently abroad, I will be back in 2 months or so.
I had a few questions regarding with the breeding:
- what kind of characteristics are being tracked and do you have a pedigree available showing the lineage of your breeding process?
- What does your breeding process look like? What methodology do you apply here e.g what are the "controlled breeding procedures" in the opening of this thread?
- How do you clone your plants?
- Have you made tissue cultures?
I am reading here that Philaris is tetraploid (4n chromosomes), and that it is self incompatible. This makes it significantly more challenging to get a stable "True to seed". "True to seed" normally means homozygosity, which would be achieved with selfing. I am guessing here you are using recurrent selection to breed at a population level?
No one seems to have developed a method to create haploids, which could be really useful in the breeding process.
I would love to get some seeds and to get in touch in the future! I am currently abroad, I will be back in 2 months or so.
Last edited:
Grasshoppers
Titanium Teammate
I am glad to hear that you like our work.
The wild populations from which the breeding line was developed are only partially documented. The original material was sourced from a variety of origins, often consisting of only a few seeds or obtained from private collections. As a result, the complete lineage is only partly known.
Seedlings from the breeding line undergo a multi-stage selection process. Their alkaloid potency is tested, and the least potent 50% are culled. This process is repeated, ensuring only plants displaying consistently high alkaloid enter the sexual reproduction.
The selected plants are then grouped according to their chemotype profiles using the following classification:
Plants are subsequently cross-pollinated within their respective groups. Where, exceptional individuals may be used for introgression between groups to combine desirable traits.
Plants can be propagated vegetatively from rhizomes when required. However, the breeding program primarily focuses on sexual reproduction to maintain genetic diversity and enable further selection.
Tissue culture has not yet been necessary.
We have not attempted to produce haploids. We have, however, considered interspecific hybridization with Phalaris arundinacea to develop a breeding line with improved cold tolerance.
The wild populations from which the breeding line was developed are only partially documented. The original material was sourced from a variety of origins, often consisting of only a few seeds or obtained from private collections. As a result, the complete lineage is only partly known.
Seedlings from the breeding line undergo a multi-stage selection process. Their alkaloid potency is tested, and the least potent 50% are culled. This process is repeated, ensuring only plants displaying consistently high alkaloid enter the sexual reproduction.
The selected plants are then grouped according to their chemotype profiles using the following classification:
Plants are subsequently cross-pollinated within their respective groups. Where, exceptional individuals may be used for introgression between groups to combine desirable traits.
Plants can be propagated vegetatively from rhizomes when required. However, the breeding program primarily focuses on sexual reproduction to maintain genetic diversity and enable further selection.
Tissue culture has not yet been necessary.
We have not attempted to produce haploids. We have, however, considered interspecific hybridization with Phalaris arundinacea to develop a breeding line with improved cold tolerance.
Can I ask a dumb question? Its where I excel
If a plant has both NN & 5MeO in it are both these extracted in a single A/B or STB so you end up with a powder mix of both?
If a plant has both NN & 5MeO in it are both these extracted in a single A/B or STB so you end up with a powder mix of both?
neurobloom
Titanium Teammate
Yes but you'd need hot pulls if you're using heptane/naptha to pull both.Can I ask a dumb question? Its where I excel
If a plant has both NN & 5MeO in it are both these extracted in a single A/B or STB so you end up with a powder mix of both?
When the non polar cools down to room temperature 5-meo-dmt crashes out as clear oil blob at the bottom. DMT will crash out later as crystals after placing in the freezer. If you want both without seperation you can just throw your pull directly into the freezer to get a highly active goo.
Ps: STB won't work on phalaris unless it's a dry alcohol tek which I don't recommend (it pulls the potent cardio active n-methyl-tyramine which otherwise would be left behind in the Aquous phase in an acid base extraction.
Highly active goo sounds appealing, How hot is hot? thanks
neurobloom
Titanium Teammate
I used 50° celcius for hexane and naptha mildly boiling. Don't use excess solvent it will trap the DMT/5-meo-dmt and won't crash or only partially Crash. It's same tek as MHRB acid base Just that hot pulls are necessary for 5-meo-dmt that's all
Thanks, also a random thought, would the Vodka TnT TEK pull everything needed out without the Acid Cook? Or is that a question too far for the moment?
neurobloom
Titanium Teammate
I don't understand why you don't like acid base? Tnt tek is just another form of alcohol Tek.
It's not like i haven't tried extracting with alcohol I did and the result is dirtier extract full of undesirable effects: sedation, confusion, tachycardia, dissociation unless you're into weird effects i suggest you keep it to acid/base.
We have bred phalaris for clean acid base profile not clean full spectrum keep that in mind.
It's not like i haven't tried extracting with alcohol I did and the result is dirtier extract full of undesirable effects: sedation, confusion, tachycardia, dissociation unless you're into weird effects i suggest you keep it to acid/base.
We have bred phalaris for clean acid base profile not clean full spectrum keep that in mind.
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