downwardsfromzero
Boundary condition
That is literally what this thread is about, try reading it. Yes, it's big. And, what gogamoga said ↑Silas said:
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Trying to improve Acacia information
- Thread starter nen888
- Start date
Woolmer
Established member
Hi Nen,
I know this is a far fetched question but it was just a thought that came up. Do you think it would be worth attempting an extraction on the galls that form on some invasive Acacias (Cyclops, Saligna) in South Africa.
A midge (Dasineura dielsi) was introduced in 2003 which feeds on the ovaries of A. Cyclops and creates a woody structure.
For A. Saligna, a rust fungus (Uromycladium tepperianum) was introduced that reduces seed and flower production and sometimes kills the plant.
Do you think these would have a major effect on the alkaloid content of the trees?
Here is a thread posted by someone on the Nexus with some detail: genetically modified acacia - First steps in Hyperspace - Welcome to the DMT-Nexus
I know this is a far fetched question but it was just a thought that came up. Do you think it would be worth attempting an extraction on the galls that form on some invasive Acacias (Cyclops, Saligna) in South Africa.
A midge (Dasineura dielsi) was introduced in 2003 which feeds on the ovaries of A. Cyclops and creates a woody structure.
For A. Saligna, a rust fungus (Uromycladium tepperianum) was introduced that reduces seed and flower production and sometimes kills the plant.
Do you think these would have a major effect on the alkaloid content of the trees?
Here is a thread posted by someone on the Nexus with some detail: genetically modified acacia - First steps in Hyperspace - Welcome to the DMT-Nexus
Woolmer
Established member
I recently attempted an extraction on A. Cyclops using 70 grams of mostly fresh phyllodes pulled with hot naphtha. I attempted to freeze precipitate after evaporating a bit till it clouded when blowing on it, but it seems like the precipitate struggled to settle down so I evaporated it all this morning. The yield is looking ok after the first pull with around 50 mg of orange/yellow goo. Unfortunately I only have a gram scale so no way of knowing exactly what the yield is.
Also, I found out that there is a suspected A. Saligna as well as A. Retinodes. I'm excited to test these species as the analysis from this thread seems very interesting.
Also, I found out that there is a suspected A. Saligna as well as A. Retinodes. I'm excited to test these species as the analysis from this thread seems very interesting.
Attachments
XJ9
Rising Star
Hoping to start work on A. Longifolia and A. Maidenii soon.
I have some phyllodes of Maidenii and phyllodes/twigs of Longifolia air drying over the next month or so.
Does anyone have any suggested tek's for me to use on these species? I don't mind goo or full-spec.
I appreciate any advice :d
I have some phyllodes of Maidenii and phyllodes/twigs of Longifolia air drying over the next month or so.
Does anyone have any suggested tek's for me to use on these species? I don't mind goo or full-spec.
I appreciate any advice :d
Woolmer
Established member
Any standard a/b will work. Cyb's hybrid tek is quite good.XJ9 said:
Yep I attempted an extraction on A. Retinodes (which I believe from a paper that I read is wrongly inventoried as A. Retinodes in South Africa but is rather A. provincialis) but yielded next to nothing. I used bark from some dead branches that were cut off a while back and lying next to the tree but it seemed heated naphtha pulls would not even pull any oils as it did not even change colour. Considering that we've have been having lots of heavy rainfall and that I have no clue how long those branches were lying there, I think it is possible that a lot of the alkaloids have been washed out with rain.XJ9 said:
An update on the cyclops extraction: I was able to yield a little bit more goo, but I believe this could be largely inactive as on an attempt at intra-nasal administration there was no burning sensation whatsoever and no effects noticed. I know this is definitely not an optimal ROA but I recently moved countries and I have nothing to smoke it with.
Finally, there is also some A. Longifolia (var. sophorae?) growing near my house that I am keen to try an extraction on so I will provide updates on that.
XJ9
Rising Star
Not sure if this has been available on the forum but I've finally gotten a hole of E. P. White's 1950's Acacia research from the New Zealand J. Sci. & Tech.
I'm pretty these were the first reports (correct me if I'm wrong) of psychoactive alkaloids being present in acacia species.
I had trouble finding these articles, but Keeper Trout came to my assistance. He provided me with scans of the original articles here:
White
However, trout contests some of White's findings - please see attached Trout's notes.
I'm pretty these were the first reports (correct me if I'm wrong) of psychoactive alkaloids being present in acacia species.
I had trouble finding these articles, but Keeper Trout came to my assistance. He provided me with scans of the original articles here:
White
However, trout contests some of White's findings - please see attached Trout's notes.
Woolmer
Established member
I have some updates on my A. Cyclops and A. Longifolia extractions.
I tried another pull on A. Cyclops after it had sat around for 10 days or so and it is still yielding lots of yellow goo. I have no idea what to think about this goo. The second I separate the naphtha from the base soup it seems these oils try to evaporate and form a ring around the naphtha pool. I struggle to get them to freeze precipitate but will try again this evening.
Regarding the A. Longifolia and A. Retinodes extractions, I believe I did not grind my bark fine enough to let the lye do its job. My coffee grinder is not optimal and I can only get it to a shredded consistency. The naphtha comes out clear whereas the A. Cycylops phyllodes (which I ground with a soup maker and a touch of vinegar also to a shredded consistency) turn the naphtha very yellow.
These results leave me very curious about the A. Cyclops trunk and root bark which I can mercilessly strip considering these species are very invasive in South Africa.
Edit: I yielded way more A. Cyclops oil from the third pull than I expected. Currently infusing it onto some imphepho 8) - will bioassay some time next week.
I tried another pull on A. Cyclops after it had sat around for 10 days or so and it is still yielding lots of yellow goo. I have no idea what to think about this goo. The second I separate the naphtha from the base soup it seems these oils try to evaporate and form a ring around the naphtha pool. I struggle to get them to freeze precipitate but will try again this evening.
Regarding the A. Longifolia and A. Retinodes extractions, I believe I did not grind my bark fine enough to let the lye do its job. My coffee grinder is not optimal and I can only get it to a shredded consistency. The naphtha comes out clear whereas the A. Cycylops phyllodes (which I ground with a soup maker and a touch of vinegar also to a shredded consistency) turn the naphtha very yellow.
These results leave me very curious about the A. Cyclops trunk and root bark which I can mercilessly strip considering these species are very invasive in South Africa.
Edit: I yielded way more A. Cyclops oil from the third pull than I expected. Currently infusing it onto some imphepho 8) - will bioassay some time next week.
Woolmer
Established member
Below are some updates on my extractions:
I bioassayed the enhanced leaf from my A. Cyclops extraction with minimal effects in a bowl but unfortunately I think I chose the wrong leaf (really harsh smoke) and I may have used too much leaf (don't own a proper scale and couldn't weigh my yield either). I will try some in a bong but I am not too hopeful about it.
Attatched below is a picture of the yellow goo. It seemed to me like a really high yield (from 50g) considering that it is a goo and covers so much of the bread plate. Surely this could not all have been plant fats/oils? Another thing which boggles me and which I mentioned before is that upon evaporation the goo started forming a fair bit outside of where the naphtha pool was if that makes sense. Notice how the yield is denser on the outside of the circle. Are these some sort of oils which try to escape but just get too heavy and fall?
This was an extraction on phyllodes from a tree in the Western Cape completely infested by the midge that was introduced for biological control.
I also did a recent extraction on trunk bark (probably over 100g dry) from an A. Cyclops in the Eastern Cape which yielded almost nothing. This tree did not have any noticable signs of ifestation. The naphtha did not change colour at all (Unlike the phyllode extraction which turned it very yellow). Does this add to the possibility that the phyllode extraction was mainly fats/oils. I have read about some unsuccessful extractions on A. Cyclops bark in this thread before.
Another thing I would like to note is that the bark extraction created a distinct layer of fats between the naphtha and soup, while the phyllodes did not.
I bioassayed the enhanced leaf from my A. Cyclops extraction with minimal effects in a bowl but unfortunately I think I chose the wrong leaf (really harsh smoke) and I may have used too much leaf (don't own a proper scale and couldn't weigh my yield either). I will try some in a bong but I am not too hopeful about it.
Attatched below is a picture of the yellow goo. It seemed to me like a really high yield (from 50g) considering that it is a goo and covers so much of the bread plate. Surely this could not all have been plant fats/oils? Another thing which boggles me and which I mentioned before is that upon evaporation the goo started forming a fair bit outside of where the naphtha pool was if that makes sense. Notice how the yield is denser on the outside of the circle. Are these some sort of oils which try to escape but just get too heavy and fall?
This was an extraction on phyllodes from a tree in the Western Cape completely infested by the midge that was introduced for biological control.
I also did a recent extraction on trunk bark (probably over 100g dry) from an A. Cyclops in the Eastern Cape which yielded almost nothing. This tree did not have any noticable signs of ifestation. The naphtha did not change colour at all (Unlike the phyllode extraction which turned it very yellow). Does this add to the possibility that the phyllode extraction was mainly fats/oils. I have read about some unsuccessful extractions on A. Cyclops bark in this thread before.
Another thing I would like to note is that the bark extraction created a distinct layer of fats between the naphtha and soup, while the phyllodes did not.
Attachments
Jagube said:
I haven't personally worked with active strains of Maidenii but nen888 has plenty of experience with it and if you get the right strain the phyllodes have good % alkaloids.. maybe 0.6% from memory? Nen?
Woolmer
Established member
I have yet to properly bioassay my A. Cyclops extract but in the meantime, I have run an A/B on at least 100g dry Acacia Erioloba trunk bark which yielded a tiny bit of yellow oil from FPing not much unlike the A. Cyclops oil. One thing to note is where I currently am there is rarely rainfall and this bark had been taken cracker dry from a tree that looks to be dying (but is not yet dead!).
Woolmer
Established member
Over the past few months, I have done quite a few extractions on a number of African and Australian acacias. Most of these extractions either ended up as a very small amount or a dirty oily yield that would not re-x and which returned a negative bioassay
My most recent extraction on Acacia Sieberiana var. Woodii has yielded something quite interesting. I only used the leaves (80g dry), removing them from the small stems which bind the leaves to be bipinnate.
I did a hybrid A/B similar to cybs tek using a hot pull with a mixture of xylene/toluene and some other hydrocarbons. Upon evaporating it I have been left with a cream seemingly crystalline substance. The weight is around 200mg. To note this was just a single 20-30ml pull.
I am very surprised at this, both the significant yield and the fact that it is more solid than gooey after evaporating the xylene/toluene.
Next step will be to re-x in some light naphtha.
I suspect the bark to have an even higher yield.
My most recent extraction on Acacia Sieberiana var. Woodii has yielded something quite interesting. I only used the leaves (80g dry), removing them from the small stems which bind the leaves to be bipinnate.
I did a hybrid A/B similar to cybs tek using a hot pull with a mixture of xylene/toluene and some other hydrocarbons. Upon evaporating it I have been left with a cream seemingly crystalline substance. The weight is around 200mg. To note this was just a single 20-30ml pull.
I am very surprised at this, both the significant yield and the fact that it is more solid than gooey after evaporating the xylene/toluene.
Next step will be to re-x in some light naphtha.
I suspect the bark to have an even higher yield.
