Centrifuge cleanup of DMT solvent pull?

[1Starway7]

Just got an idea!

Question to those here who have more knowlege than me about this...

Could one skip the ...easy cleanup... and other time consuming methods of.... defatting/and cleaning impuritys from a ....solvent pull .....containing dmt molecules buy simply putting the solvent pull into a high speed Centrifuge?

It seems this could work ...if the impuritys are a different density than the dmt molecules! Is this posible? has it ever been done this way?/

 

[Transform]

Just got an idea!

Question to those here who have more knowlege than me about this...

Could one skip the ...easy cleanup... and other time consuming methods of.... defatting/and cleaning impuritys from a ....solvent pull .....containing dmt molecules buy simply putting the solvent pull into a high speed Centrifuge?

It seems this could work ...if the impuritys are a different density than the dmt molecules! Is this posible? has it ever been done this way?/

Well, if ultracentrifuges can separate isotopes with about 3% difference in mass (although that's more correctly a gas centrifuge), polymers should be a doddle along with aqueous droplets, although this last one is the only one I think would get significantly separated in a normal centrifuge.

Could be worth a bit of experimentation if you have access to the equipment.

 

[1Starway7]

Well, if ultracentrifuges can separate isotopes with about 3% difference in mass (although that's more correctly a gas centrifuge), polymers should be a doddle along with aqueous droplets, although this last one is the only one I think would get significantly separated in a normal centrifuge.

Could be worth a bit of experimentation if you have access to the equipment.

I just ran into a used centrifuge...at a flea market today.... its an older one...it feels like it weighs 40 or 50 lbs! .... Its marked ....... [university of colorado med center .[ lab].......Must be a good one.. it has 6 metal tubes to put specimens in .... some of their older equipment i guess...

When i turn it on it starts slow ... but when turned up it goes to warp speed! so fast its a blurr! ....its not a home made device...

I believe i can just buy clear plastic or glass sample tubes ..that fit]... and remove the larger metal tubes...


Ill send a picture of the machine...

The last smaller picture looks very much like the machine i have with the slide speed control....zoom picture to read words..


i read this...


hese are results for can a fast centrifuge separate a drug from powdered bark and remove plant fats in a naphtha solvent
Search instead for can a fast centrifuge seperate a drug from powdered bark and remove plant fats in a napatha solvent




computer generated answer....
AI Overview



Yes, a fast centrifuge can be used in the process to separate a drug from powdered bark and remove plant fats in a naphtha solvent, but it is not a one-step solution
. Centrifugation is a mechanical separation technique that complements the solvent extraction process by speeding up the physical separation of components.

How a centrifuge helps
The solvent extraction process works by dissolving desired compounds, like a drug, along with undesirable ones, like fats, into a solvent. Because these compounds have different densities and properties, a centrifuge can accelerate their separation.

1. Separating solid from liquid: After soaking the powdered bark in naphtha, the mixture will contain solid plant matter and the naphtha solvent with dissolved compounds. The first use for a centrifuge is to spin this mixture at high speed, forcing the solid, heavier bark particles to the bottom of the container. This allows the liquid extract to be decanted (poured off) or siphoned, separating it from the bulk of the solid plant waste.
2. Separating fats from the extract: Plant fats (lipids) have a different density than the naphtha solvent and the drug compound. The specific effect of centrifugation depends on the relative densities:
   • If the fats are less dense than the naphtha and the drug, they will float to the top of the liquid mixture during centrifugation.
   • If the fats are more dense, they will form a pellet or a layer at the bottom of the container.
3. Breaking emulsions: In some extractions, fats can form a stable emulsion with the solvent, making the mixture cloudy and difficult to separate. A fast centrifuge can apply sufficient force to break this emulsion, allowing the distinct layers to separate for easier collection.

Limitations of using only a centrifuge
While powerful, a centrifuge is not a magic bullet. For a complex mixture like powdered bark extract, other purification steps are necessary to get a pure final product.

• Non-selective extraction: Naphtha, being a non-polar solvent, will dissolve all non-polar compounds from the bark, including the desired drug, fats, waxes, and other lipids. The centrifuge separates based on density, but if the drug and the fats have similar densities, centrifugation alone will not be effective at isolating them.
• Other methods needed: Other techniques, such as chromatography, fractional freezing, or solvent-solvent extraction, are needed to fully purify the drug from the fats and other compounds with similar densities.

Summary of the process
A more complete and realistic extraction and purification process might look like this:

1. Solvent Extraction: Soak the powdered bark in naphtha to dissolve the target drug and other non-polar compounds, including fats.
2. Initial Separation: Centrifuge the bark/naphtha mixture. The powdered bark pellets at the bottom, and the liquid containing the drug and fats can be collected.
3. Fat Removal: Subject the liquid extract to a second centrifugation step to separate the fats, which will either float or pellet out depending on their density.
4. Further Purification: Use a more selective method, such as liquid-liquid extraction with a different solvent or chromatography, to isolate the drug from any remaining impurities.

• A single centrifugation method for isolating fat droplets from ...
  We utilized two properties conferred by the fat core of FDs to fractionate them away from the cytosol and sedimentable organelles. First, because FDs lack an aq...
  
  
  
  National Institutes of Health (NIH) | (.gov)
  
  
  
• Techniques for extraction and isolation of natural products
  Apr 17, 2018 — Extraction is the first step to separate the desired natural products from the raw materials. Extraction methods include solvent extraction, distillation method...
  
  
  
  National Institutes of Health (NIH) | (.gov)
  
  
  
• Comparison of Techniques and Solvents on the Antimicrobial ...
  Four extraction methods (solid-liquid, ultrasound, Soxhlet, and microwave) and six solvents (water, methanol, ethanol, acetone, dichloromethane, and hexane) wer...
  
  
  
  National Institutes of Health (NIH) | (.gov)
  
  

Show all











































[[can a centrifuge be used to separate out impurities from an organic drug??

Yes, a centrifuge can be used to separate impurities from an organic drug, particularly when the impurities have a different density or size than the desired product
. The effectiveness of centrifugation depends on the state of the substances you want to separate—specifically, whether the impurities are solid particles suspended in a liquid or are part of a mixture of different density fluids.

How it works for organic drug purification
The process is an excellent option for certain types of separation and is often used in combination with other purification methods.

• Separation based on density: A centrifuge separates components in a mixture by spinning them at high speed. The resulting centrifugal force causes the denser particles to move toward the bottom of the container, forming a pellet, while the lighter components remain in the liquid supernatant.
• Separating solids from liquids: If your organic drug is dissolved in a solvent and the impurities are undissolved solids (like salts, catalysts, or precipitates from a crystallization), a centrifuge can spin these solid particles to the bottom of the tube for easy removal.
• Purifying crystallized products: In pharmaceutical manufacturing, centrifuges are used to separate crystallized drug substances from the "mother liquor," which contains remaining impurities and solvent. This is a critical step for isolating the bulk drug.
• Removing particulate matter: For injectable drugs or other formulations that require high purity, centrifuges can remove fine particulate matter that is too small for standard filtration.

Limitations for organic drugs
Centrifugation is a highly effective, but not universal, purification technique. Its usefulness is dependent on the properties of the mixture.

• Solubility and density are key: The method is most effective when the desired drug and the impurities have significant differences in density. If the impurities and the drug have similar densities, a centrifuge alone may not be able to separate them.
• Does not remove dissolved impurities: Centrifugation is not effective for removing impurities that are dissolved in the same solvent as the drug. It can only separate components that are physically distinct (e.g., a solid from a liquid).
• Other techniques may be needed: Many organic synthesis and purification processes require other techniques to get a high-purity product. For example, after an initial centrifugation to remove solid particles, additional methods like chromatography, distillation, or recrystallization may be necessary to remove dissolved impurities.

Combined purification techniques
Centrifugation is often a single step in a broader purification process.

• Centrifugal filtration: This technique combines the use of a semi-permeable membrane with centrifugal force to separate particles based on size and molecular weight.
• Recrystallization and centrifugation: For solid products, an organic drug is often dissolved, crystallized to precipitate the pure product, and then centrifuged to isolate the solid crystals from the liquid impurities.
• Centrifugation for sample preparation: In analytical chemistry, a centrifuge is frequently used to prepare samples for more advanced analysis techniques like mass spectrometry or chromatography, ensuring the sample is free of interfering particulate matter.

AI responses may include mistakes. Learn more

16 sites

• Centrifuges Used in Pharmaceutical Manufacturing
  Feb 24, 2025 — Centrifuges Used in Pharmaceutical Manufacturing. ... The pharmaceutical industry relies on precise and efficient centrifuges to meet the exacting standards of ...
  
  
  
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• Purification Via Centrifugation: Method for Separating ...
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[1Starway7]

I just ran into a used centrifuge...at a flea market today.... its an older one...it feels like it weighs 40 or 50 lbs! .... Its marked ....... [university of colorado med center .[ lab].......Must be a good one.. it has 6 metal tubes to put specimens in .... some of their older equipment i guess...

When i turn it on it starts slow ... but when turned up it goes to warp speed! so fast its a blurr! ....its not a home made device...

I believe i can just buy clear plastic or glass sample tubes ..that fit]... and remove the larger metal tubes...


Ill send a picture of the machine...

The last smaller picture looks very much like the machine i have with the slide speed control....zoom picture to read words..


i read this...


hese are results for can a fast centrifuge separate a drug from powdered bark and remove plant fats in a naphtha solvent
Search instead for can a fast centrifuge seperate a drug from powdered bark and remove plant fats in a napatha solvent




computer generated answer....
AI Overview



Yes, a fast centrifuge can be used in the process to separate a drug from powdered bark and remove plant fats in a naphtha solvent, but it is not a one-step solution
. Centrifugation is a mechanical separation technique that complements the solvent extraction process by speeding up the physical separation of components.

How a centrifuge helps
The solvent extraction process works by dissolving desired compounds, like a drug, along with undesirable ones, like fats, into a solvent. Because these compounds have different densities and properties, a centrifuge can accelerate their separation.

1. Separating solid from liquid: After soaking the powdered bark in naphtha, the mixture will contain solid plant matter and the naphtha solvent with dissolved compounds. The first use for a centrifuge is to spin this mixture at high speed, forcing the solid, heavier bark particles to the bottom of the container. This allows the liquid extract to be decanted (poured off) or siphoned, separating it from the bulk of the solid plant waste.
2. Separating fats from the extract:Plant fats (lipids) have a different density than the naphtha solvent and the drug compound. The specific effect of centrifugation depends on the relative densities:
   • If the fats are less dense than the naphtha and the drug, they will float to the top of the liquid mixture during centrifugation.
   • If the fats are more dense, they will form a pellet or a layer at the bottom of the container.
3. Breaking emulsions: In some extractions, fats can form a stable emulsion with the solvent, making the mixture cloudy and difficult to separate. A fast centrifuge can apply sufficient force to break this emulsion, allowing the distinct layers to separate for easier collection.

Limitations of using only a centrifuge
While powerful, a centrifuge is not a magic bullet. For a complex mixture like powdered bark extract, other purification steps are necessary to get a pure final product.

• Non-selective extraction: Naphtha, being a non-polar solvent, will dissolve all non-polar compounds from the bark, including the desired drug, fats, waxes, and other lipids. The centrifuge separates based on density, but if the drug and the fats have similar densities, centrifugation alone will not be effective at isolating them.
• Other methods needed: Other techniques, such as chromatography, fractional freezing, or solvent-solvent extraction, are needed to fully purify the drug from the fats and other compounds with similar densities.

Summary of the process
A more complete and realistic extraction and purification process might look like this:

1. Solvent Extraction: Soak the powdered bark in naphtha to dissolve the target drug and other non-polar compounds, including fats.
2. Initial Separation: Centrifuge the bark/naphtha mixture. The powdered bark pellets at the bottom, and the liquid containing the drug and fats can be collected.
3. Fat Removal: Subject the liquid extract to a second centrifugation step to separate the fats, which will either float or pellet out depending on their density.
4. Further Purification: Use a more selective method, such as liquid-liquid extraction with a different solvent or chromatography, to isolate the drug from any remaining impurities.

• A single centrifugation method for isolating fat droplets from ...
  We utilized two properties conferred by the fat core of FDs to fractionate them away from the cytosol and sedimentable organelles. First, because FDs lack an aq...
  
  
  
  National Institutes of Health (NIH) | (.gov)
  
  
  
• Techniques for extraction and isolation of natural products
  Apr 17, 2018 — Extraction is the first step to separate the desired natural products from the raw materials. Extraction methods include solvent extraction, distillation method...
  
  
  
  National Institutes of Health (NIH) | (.gov)
  
  
  
• Comparison of Techniques and Solvents on the Antimicrobial ...
  Four extraction methods (solid-liquid, ultrasound, Soxhlet, and microwave) and six solvents (water, methanol, ethanol, acetone, dichloromethane, and hexane) wer...
  
  
  
  National Institutes of Health (NIH) | (.gov)
  

Show all











































[[can a centrifuge be used to separate out impurities from an organic drug??

Yes, a centrifuge can be used to separate impurities from an organic drug, particularly when the impurities have a different density or size than the desired product
. The effectiveness of centrifugation depends on the state of the substances you want to separate—specifically, whether the impurities are solid particles suspended in a liquid or are part of a mixture of different density fluids.

How it works for organic drug purification
The process is an excellent option for certain types of separation and is often used in combination with other purification methods.

• Separation based on density: A centrifuge separates components in a mixture by spinning them at high speed. The resulting centrifugal force causes the denser particles to move toward the bottom of the container, forming a pellet, while the lighter components remain in the liquid supernatant.
• Separating solids from liquids: If your organic drug is dissolved in a solvent and the impurities are undissolved solids (like salts, catalysts, or precipitates from a crystallization), a centrifuge can spin these solid particles to the bottom of the tube for easy removal.
• Purifying crystallized products: In pharmaceutical manufacturing, centrifuges are used to separate crystallized drug substances from the "mother liquor," which contains remaining impurities and solvent. This is a critical step for isolating the bulk drug.
• Removing particulate matter: For injectable drugs or other formulations that require high purity, centrifuges can remove fine particulate matter that is too small for standard filtration.

Limitations for organic drugs
Centrifugation is a highly effective, but not universal, purification technique. Its usefulness is dependent on the properties of the mixture.

• Solubility and density are key: The method is most effective when the desired drug and the impurities have significant differences in density. If the impurities and the drug have similar densities, a centrifuge alone may not be able to separate them.
• Does not remove dissolved impurities: Centrifugation is not effective for removing impurities that are dissolved in the same solvent as the drug. It can only separate components that are physically distinct (e.g., a solid from a liquid).
• Other techniques may be needed: Many organic synthesis and purification processes require other techniques to get a high-purity product. For example, after an initial centrifugation to remove solid particles, additional methods like chromatography, distillation, or recrystallization may be necessary to remove dissolved impurities.

Combined purification techniques
Centrifugation is often a single step in a broader purification process.

• Centrifugal filtration: This technique combines the use of a semi-permeable membrane with centrifugal force to separate particles based on size and molecular weight.
• Recrystallization and centrifugation: For solid products, an organic drug is often dissolved, crystallized to precipitate the pure product, and then centrifuged to isolate the solid crystals from the liquid impurities.
• Centrifugation for sample preparation: In analytical chemistry, a centrifuge is frequently used to prepare samples for more advanced analysis techniques like mass spectrometry or chromatography, ensuring the sample is free of interfering particulate matter.

AI responses may include mistakes. Learn more

16 sites

• Centrifuges Used in Pharmaceutical Manufacturing
  Feb 24, 2025 — Centrifuges Used in Pharmaceutical Manufacturing. ... The pharmaceutical industry relies on precise and efficient centrifuges to meet the exacting standards of ...
  
  
  
  www.westernstates.com
  
  
  
  
  
  
• Purification Via Centrifugation: Method for Separating ...
  Dec 23, 2022 — Purification Via Centrifugation: A Quick and Efficient Method for Separating Substances * When it comes to separating substances, there are several methods that...
  
  
  
  Zuiveringstechnieken
  
  
  
  
  
  
• A Review on Centrifugation in the Pharmaceutical Industry
  A Review on Centrifugation in the Pharmaceutical Industry. ... Correspondence to: Stephen Olaribigbe Majekodunmi, Department of Pharmaceutics and Pharmaceutical...
  
  
  
  Scientific & Academic Publishing
  
  
  

Show all

 

[1Starway7]

Another posibility is the use of centrifuge .. to clean up and purify syrian rue....and other organics..



AI Overview OK! i just love talking to ..[Artificial intelegence]



Based on a search of scientific literature, a fast centrifuge can be used as a final cleanup step to separate drug-containing acidic water from remaining powdered bark
. However, centrifugation alone cannot perform the entire drug extraction process as described. Here is a breakdown of how the process would likely work:
Step 1: Boiling the drug out

• Boiling powdered bark in an acidic water solution is the standard method for extracting alkaloids (the active drug compounds) into the water....I agree
• The acidic environment converts the alkaloids into their salt form, which is soluble in water, thus drawing the drug out of the solid plant matter......I agree

Step 2: Removing the bark.....

• After boiling, the mixture contains the drug dissolved in the acidic water, plus the solid bark material.....
• Im aware of this fact...
• 
  
• While a centrifuge can be used to separate these two components, it is typically the final purification step. Initial separation is often done through simpler methods like filtration (such as pouring the mixture through a filter) to remove the bulk of the solids....

.....Simpler methods like using coffie filters are also slow and messy

• 
  
• [[.i already know this fact.....i will remove the bulk of the powdered bark then. ... put it in a strainer and catch the majority of the acidic water in container...]].
• .....BUT!! doing this still leaves a lot of very fine impuritys in the acidic soup!!

• 
  
• YES!!..I agree!!........A centrifuge can then be used on the filtered liquid to remove any remaining, fine plant particles that did not settle out.
• Why not let the centrifuge remove ...very cleanly.... the remaining thousands of tiny impuritys from the acidic water resulting in a more clean product....

Step 3: Removing the drug from the acidic water

• At this point, the drug is dissolved in the acidic water. A fast centrifuge cannot remove the drug from the water because it is not a solid particle to be separated by centrifugal force....
• [.YES i understand this fact..]
• 
  
• 
  
• Instead, additional chemical processing is required. This often involves the use of a non-polar solvent or other chemical processes.
• To get a final solid product, the drug can be precipitated from the water after other steps have been completed.
• 
  
• 
  
• [[However, no "freebasing" is needed.]].... Freebasing is a separate chemical process used to convert a drug back from its salt form to a pure, smokable base, and is not a necessary part of the extraction and purification process to recover the drug. ...
• 
  
• [This info above sounds great...if freebasing is not needed to bring it back to base.... Then ... how it this done straight from a salt of DMT?

In summary:
The centrifuge cannot accomplish the entire process alone, and freebasing is not required. The proper procedure would involve:... Understood!..

1. Boiling the bark in acidic water to extract the drug.
2. Filtering the coarse solids from the liquid.
3. Centrifuging the liquid to remove any remaining fine particles.
4. Chemically processing the liquid to isolate the drug. ...
5. .{if you dont use lye to freebase the drug.....is there something ....[ cleaner/safer]... to use... that will cleanly release the drug [DMT] from the acidic water??
6. I ask this because so far...nothing harsh or unsafe has been used in the steps above.....would like the product to be clean....

can anyone add an opinion? about something safer than using lye to freebase product?

• 
  
  
  

 

[Transform]

No, a centrifuge is not a substitute for base! The AI was doing so well up to the point of suggesting that freebasing was unnecessary, falling down at least in the manner of failing to provide further context.

Freebasing is a separate chemical process used to convert a drug back from its salt form to a pure, smokable base, and is not a necessary part of the extraction and purification process to recover the drug.

I think the highlighted section ought to read "not necessarily a part". There also appears to be text missing/omitted from your quotes of the machine output, so it's hard to tell whether there would have been further, possibly important nuances of context provided.

It could, however, be quite useful having a centrifuge available for clarification of harmala brews or tannin-rich mimosa or acacia brews.

One really important issue is that you must be fully versed in all the relevant safety protocols for use of a centrifuge, and also bear in mind that the rotor could fail catastrophically if it has significantly exceeded its design life.

 

[1Starway7]

No, a centrifuge is not a substitute for base! The AI was doing so well up to the point of suggesting that freebasing was unnecessary, falling down at least in the manner of failing to provide further context.

I think the highlighted secton my pcion ought to read "not necessarily a part". There also appears to be text missing/omitted from your quotes of the machine output, so it's hard to tell whether there would have been further, possibly important nuances of context provided.

It could, however, be quite useful having a centrifuge available for clarification of harmala brews or tannin-rich mimosa or acacia brews.

One really important issue is that you must be fully versed in all the relevant safety protocols for use of a centrifuge, and also bear in mind that the rotor could fail catastrophically if it has significantly exceeded its design life.

Yes talking to [artifical Intelegence]... can get weird..
[...AI].... knows not to tell you everything !....this shows it has the abulity to withold info...... because in some states its illeagle ..... [but DMT/ schrooms/ meccaline and even lsd ... is decriminalized in my state...

At times [asking questions] that.... AI... responds to ..its much like talking to another person....

If i asked ...AI...will ...sodium carbonate...work ok to replace LYE to freebase a certain drug?...it wont tell me! .... and says its illeagle and very dangorus!

Its almost like AI is waiting for my next question!......on my pc i cant ask a question without it answering me ...un invited!

I guess i could ask ... AI... to go away?

 

[1Starway7]

Yes talking to [artifical Intelegence]... can get weird..
[...AI].... knows not to tell you everything !....this shows it has the abulity to withold info...... because in some states its illeagle ..... [but DMT/ schrooms/ meccaline and even lsd ... is decriminalized in my state...

At times [asking questions] that.... AI... responds to ..its much like talking to another person....

If i asked ...AI...will ...sodium carbonate...work ok to replace LYE to freebase a certain drug?...it wont tell me! .... and says its illeagle and very dangorus!

Its almost like AI is waiting for my next question!......on my pc i cant ask a question without it answering me ...un invited!

I guess i could ask ... AI... to go away?

First small test.. because i didnt have time to powder and boil any acacia bark yet...
I made two equal sized plastic containers and .... [and for machine ballence]. i leveled it on a flat surface before running it.....i put an equal amount of ...[uncrushed]...rue seed in them... then put equal amount of ever clear high proof alcohol ..to help actives leach out faster..
Im going to let the machine spin 7000 rpm for 5 minutes and check what the vial looks like...

ok its been 8 or more minutes and the viles have the usual turquois color under black light... Aazingly! i can actually make out the color ...without a black light]...!!..and all the un crushed rue seed is pushed over on one side ..and i noticed the alcohol is super clear and clean looking not even a tiny particle in it!
This was the first time being able see the turquois color with my bare eyes!

 

[Transform]

This was the first time being able see the turquois color with my bare eyes!

I find this happens if the harmala solution is kept in a shaded area where diffuse light is still able to reach it. Fluorescence occurs sufficiently to be observed just as you say. Was the device near a window with daylight, or an old-fashioned fluorescent tube?

What was the colour of the solution (besides the turquoise fluorescence) when viewed outside of the centrifuge? The fluorescence - especially the turquoise - can be visible at fairly low concentrations, shifting to a much greener hue at higher concentrations.

Also, the turquoise glow can appear 'transparent' because of this, whereas the green glow from the more concentrated solutions often can be strong enough to appear opaque even in otherwise crystal clear solutions. The bright emission blanks out the background.

These effects can be used as a very rough means of estimating ballpark harmala concentration.


All the same, it's great to hear of a preliminary result!

 

[Sakkadelic]

How about starting with a reduced and filtered acidic dmt tea, basify, then use the centrifuge to separate the freebase dmt, decant then add weakly basic water and run the centrifuge again, repeat until the water stays clear. Similar to harmala freebase water washes where the freebase settles by gravity alone. It would probably work better with backsalted dmt from NPS.

 

[1Starway7]

How about starting with a reduced and filtered acidic dmt tea, basify, then use the centrifuge to separate the freebase dmt, decant then add weakly basic water and run the centrifuge again, repeat until the water stays clear. Similar to harmala freebase water washes where the freebase settles by gravity alone. It would probably work better with backsalted dmt from NPS.

Worth a try...but from what ive learned a centrifuge seperates material on the basis of.. [ density] ... the more dense material gets forced away from the lighter material and there for is seperated....
In this one little test using ... uncrushed rue seed ...it simply pushed all the seed to bottom of tube leaving an extreemly cleare looking everclear high proof alcohol. and i only ran the centrifuge for 12 minutes...

When i say clear ...it was very very clean !... it probibly removed particles unseeable to the human eye... Not positive of the RPMs my machine is running ..but the smaller picture i put on line .... looks identicle to mine... and is capable of 7000 RPMS!... which is considered high.. but not the highest. ....mine is either 5000rpms or 7000rpms
.The faster centrifuges can actually sepprate very tiny things down to the cellular level....these faster machines can even pull out bacteria or viruses to be studied..

 

[1Starway7]

I find this happens if the harmala solution is kept in a shaded area where diffuse light is still able to reach it. Fluorescence occurs sufficiently to be observed just as you say. Was the device near a window with daylight, or an old-fashioned fluorescent tube?

What was the colour of the solution (besides the turquoise fluorescence) when viewed outside of the centrifuge? The fluorescence - especially the turquoise - can be visible at fairly low concentrations, shifting to a much greener hue at higher concentrations.

Also, the turquoise glow can appear 'transparent' because of this, whereas the green glow from the more concentrated solutions often can be strong enough to appear opaque even in otherwise crystal clear solutions. The bright emission blanks out the background.

These effects can be used as a very rough means of estimating ballpark harmala concentration.


All the same, it's great to hear of a preliminary result!

The liquid i used was clear because it was everclear alcohol ...the color had kind of a greenish/bluish tint after mixed with whole uncrushed seed... What impressed me was the clearness of the liquid ..very clean!

I was thinking of trying the boiling powdered bark with no acid added [because in south america the natives dont acidify their water ..they boil water with leaves and bark]... ...[im only using two tubes now but the machine can hold 6 tubes... ill try distilled water with powdered bark...

 

[Sakkadelic]

Worth a try...but from what ive learned a centrifuge seperates material on the basis of.. [ density] ... the more dense material gets forced away from the lighter material and there for is seperated....

Yes, I couldn't find an exact number for the density of freebase dmt but it is estimated to be slightly above that of water according to gpt 1.05–1.10 g/cm³.

From my experience observing the basification of dmt solutions, it tends to stay suspended, it does not seem to float or sink, maybe with a centrifuge it will be forced down

 

[Transform]

Yes, I couldn't find an exact number for the density of freebase dmt but it is estimated to be slightly above that of water according to gpt 1.05–1.10 g/cm³.

From my experience observing the basification of dmt solutions, it tends to stay suspended, it does not seem to float or sink, maybe with a centrifuge it will be forced down

It's highly likely that water with bark and base ends up having a similar density to that of DMT. The best bet would be to increase the base soup's density even more - maybe potassium hydroxide would help with this (and it's more accessible than caesium hydroxide )

 

[Transform]

The liquid i used was clear because it was everclear alcohol ...the color had kind of a greenish/bluish tint after mixed with whole uncrushed seed... What impressed me was the clearness of the liquid ..very clean!

How does this compare with the colour of your usual everclear extracts? Do you have any idea what the concentration of the centrifuged tincture might be?

 

[1Starway7]

Yes, I couldn't find an exact number for the density of freebase dmt but it is estimated to be slightly above that of water according to gpt 1.05–1.10 g/cm³.

From my experience observing the basification of dmt solutions, it tends to stay suspended, it does not seem to float or sink, maybe with a centrifuge it will be forced down

I believe.. You have to extract the dmt first out of the bark into... a liquid or solvent... before putting into centrifuge........ you do this by boiling the powdered bark untill it migrates into the acidified water..in a salt form ...and if your using acidified water ....



In [acidified water state]... the dmt is NOT.. in the heavier dense freebase form....its much lighter as its desolved in the acidic water...

I understand that ....the dense heavier stuff.... mostly bark particles and other dense micro impuritys will be forced into the bottom of the tube... seperate from the acidic water..

Then later basify the acidic water... or non acidic water later? with lye..or better choice something a little safer or weaker possibly ...[sodium carbonate]?...
although...I admit ... lye does a good job of basifying!.......it still costic stuff... i hope this makes sense...

 

[1Starway7]

How does this compare with the colour of your usual everclear extracts? Do you have any idea what the concentration of the centrifuged tincture might be?

compares... .About the same..... only it looks so much cleaner and clearer....no particles of any kind visible...

 

[Transform]

compares... .About the same..... only it looks so much cleaner and clearer....no particles of any kind visible...

So it's yellow, like normal rue brews? Because if it is, it makes me wonder if the forces in the centrifuge help to squeeze harmalas out of the seeds. Would an uncentrifuged seed tincture develop as much colour during that short time span?

 

[1Starway7]

So it's yellow, like normal rue brews? Because if it is, it makes me wonder if the forces in the centrifuge help to squeeze harmalas out of the seeds. Would an uncentrifuged seed tincture develop as much colour during that short time span?

not shure about the color differencre.... i did use high proof alcohol in hopes of extracting it ..using whole seeds...i figure i may have only got the actives from outside the seed?
If the seeds are crushed... its going to be more color and more density...

I did ready about 45 grams of powdered bark in acidic water...for next trial with centrifuge.. but first.. i will have to boil the bark soup to move the actives into the acidic water..

Centrifugation in Pharmaceuticals: Objectives, Principle, and Applications

Centrifugation is a widely used technique in pharmaceuticals for various purposes, including the extraction of drugs from powdered bark. The principle of centrifugation is based on sedimentation, where particles suspended in a medium are separated under the influence of centrifugal force. This method is particularly useful for isolating different components within a liquid mixture, such as suspensions or emulsions. In the context of drug extraction, centrifugation can help in separating the active ingredients from the powdered bark, ensuring a pure and effective drug product.

Louisiana Tech University

 

[1Starway7]

not shure about the color differencre.... i did use high proof alcohol in hopes of extracting it ..using whole seeds...i figure i may have only got the actives from outside the seed?
If the seeds are crushed... its going to be more color and more density...

I did ready about 45 grams of powdered bark in acidic water...for next trial with centrifuge.. but first.. i will have to boil the bark soup to move the actives into the acidic water..

Centrifugation in Pharmaceuticals: Objectives, Principle, and Applications

Centrifugation is a widely used technique in pharmaceuticals for various purposes, including the extraction of drugs from powdered bark. The principle of centrifugation is based on sedimentation, where particles suspended in a medium are separated under the influence of centrifugal force. This method is particularly useful for isolating different components within a liquid mixture, such as suspensions or emulsions. In the context of drug extraction, centrifugation can help in separating the active ingredients from the powdered bark, ensuring a pure and effective drug product.
Louisiana Tech University

after boiling 45 grams of powdered bark quite a while in acidified water...i removed most of the spent bark in a strainer ...then loaded soup in two viles in the Centrifuge and spun them for about 5 minutes...below pictures are the results...
The machine seperated ALL of the fine particles...i mean ALL of them!...and now i have a very ..clear /clean...red colored acidic water?
I asume the dmt salt is in the red acidified water..?
I did not basify anything yet....
Magnify..and see the tightly packed sedement in bottom of flasks! there should?... be dmt salt in this red liquid...i doubt its in the fine tightly packed bark sedement on bottom of flask?

now i think i have a very clean red colored acidic water that should contain a little dmt salt....what to try next?

normaly .... i think one would add 50 percent clean acidified water to ....50 percent unwashed dmt pull ...to do an ....easy AB cleanup .....then shake many times and let fats and impuritys seperate ect......picture 1189 and picture 1190 are pictures of the first two viles that were spun ...has a nice red wine look!

Im doing things different this time ..the red liquid looks very clean except for the red dye from the bark...