Hmm honestly I didn´t pay attention, I was trying to do a really quick extraction so I took some shortcuts, I didnt measure the amount of sodium carb so it was prob really concentrated solution, and after wash I decanted and just used it to extract straight away. If you use only a dilute solution and you freeze to remove excess water after, you´re prob not gonna have this issue in a significant way, but I think its important to keep in mind, a second water-only wash is a very simple addition to the work flow and would def prevent this issue.
if this does happen to anybody, sodium citrate is practically insoluble in alcohols including methanol, Im not sure mesc citrate solubility in ethanol or IPA, but its def soluble in methanol, so I separated it this way, soaking in methanol to dissolve mesc citrate and leave sodium citrate behind, but I have a rotovap to make sure no methanol is left when I evap it, I would avoid using it otherwise... But if mesc citrate is at soluble in IPA or ethanol one could use that instead.. Or just re-a/b and issue is solved. But better avoid it in the first place
if this does happen to anybody, sodium citrate is practically insoluble in alcohols including methanol, Im not sure mesc citrate solubility in ethanol or IPA, but its def soluble in methanol, so I separated it this way, soaking in methanol to dissolve mesc citrate and leave sodium citrate behind, but I have a rotovap to make sure no methanol is left when I evap it, I would avoid using it otherwise... But if mesc citrate is at soluble in IPA or ethanol one could use that instead.. Or just re-a/b and issue is solved. But better avoid it in the first place