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TLC Kits are out - link here!
- Thread starter endlessness
- Start date
He said "probably 3 weeks" for the beginning of European shipping.
In the meanwhile, I've been told that through the lunarlaboratories page, shipping from USA to europe works fine too and shipping shouldn't be too expensive.
Either way I will post here once they finally start shipping from Europe
In the meanwhile, I've been told that through the lunarlaboratories page, shipping from USA to europe works fine too and shipping shouldn't be too expensive.
Either way I will post here once they finally start shipping from Europe
Running Bear
Rising Star
Brilliant! :thumb_up:
Thank you endlessness!
Thank you endlessness!
downwardsfromzero
Boundary condition
My eyes are now officially being kept peeled!
plantatron
Rising Star
I am also very much interested in purchasing a TLC kit within Europe.
I guess ordering from US would work, but every package I got from the US in the last 5 years went through customs and they ask questions and randomly open the packages in order to check the validity of the receipt or in case they don't understand the description of the contents.
I do have several Acacias that are about 1 year old and which I grew from seed here in northern Europe (think many grey and rainy days and not too much sun for them) which I would love to test and share results, of course.
Any updates on when the European shipping will be available ?
I guess ordering from US would work, but every package I got from the US in the last 5 years went through customs and they ask questions and randomly open the packages in order to check the validity of the receipt or in case they don't understand the description of the contents.
I do have several Acacias that are about 1 year old and which I grew from seed here in northern Europe (think many grey and rainy days and not too much sun for them) which I would love to test and share results, of course.
Any updates on when the European shipping will be available ?
So, the european shipping has started.. They are finishing up some last details to have it done by default to all European residents.. I was told that in the meanwhile, please when you make your order, write on the order comments "European Shipping" and it will already be shipped from within europe
plantatron
Rising Star
This is awesome news! :thumb_up:
Aum_Shanti
Esteemed member
Would be very interested in testing some phalaris, before doing extensive *C/MS.
Would this test kit work to distinguish DMT, 5-MeO-DMT, NMT, 5-MeO-NMT, Gramine by having them different rf-values (different spots).
E.g. I read in trout's stuff that e.g. Acetic acid (2N) on Silica would work good to distinguish DMT from 5-MeO-DMT (rf 0.5 vs 0.6), but no rf values are indicated for the other stuff.
Whereas for most other systems the differentiation is very poor. That's why I ask.
I couldn't really find detailed information about the TLC-material used in these kits. Paper? Silica?
You said, to use the rf calculator on tlconscious.me. But unfortunately this calculator doesn't work for me (I only get a blank white box on my Firefox).
Thanks for any answer.
Would this test kit work to distinguish DMT, 5-MeO-DMT, NMT, 5-MeO-NMT, Gramine by having them different rf-values (different spots).
E.g. I read in trout's stuff that e.g. Acetic acid (2N) on Silica would work good to distinguish DMT from 5-MeO-DMT (rf 0.5 vs 0.6), but no rf values are indicated for the other stuff.
Whereas for most other systems the differentiation is very poor. That's why I ask.
I couldn't really find detailed information about the TLC-material used in these kits. Paper? Silica?
You said, to use the rf calculator on tlconscious.me. But unfortunately this calculator doesn't work for me (I only get a blank white box on my Firefox).
Thanks for any answer.
JustAnotherHuman
You create your own reality
This is great stuff you're doing here endlessness! The community needs it!
Many thanks!:thumb_up:
Many thanks!:thumb_up:
The Rf values differ for various solvent systems. The values given by the calculator are valid only for the solvent system in the tlc testing kit, I assume.Aum_Shanti said:
The tlc testing kit comes with testing reagents that give color reactions with many alkaloids. You can get many other reagents if the one you need doesn't come included with the kit. With the reagents you don't depend on precalculated Rf values.
IIRC it's silica gel with fluorescent indicator, so you can see where the spots are under uv illumination and mark them with pencil. You can then test those spots with reagents.Aum_Shanti said:
It doesn't work for me either. So I had a look at the html source and found this link on the page: https://chat.dmt-nexus.me/tlc_ratio.aspx . It works. Long live html 1.0!Aum_Shanti said:
Did you read this post: Drug testing reagent results videos and TLC spot height calculator ?
Aum_Shanti
Esteemed member
Thanks a lot! Especially for that calculator link. That helped a lot to see how close they are together.
It seems they are quite close together (5-MeO-DMT and DMT), but probably they can be differentiated. Anyone any experience if this is the case with the testkit (can one differentiate the spots)?
E.g. How long are the TLC-Plates? And how high would caffeine be, when the liquid reaches the top?
If they are silica, then I could try to use them with Acetic Acid if I wanted a better discrimination between DMT and 5-MeO-DMT.
I had a look in the database endlessness linked in the thread you mentioned. But as it seems the delivered reagents cannot discriminate on the TLC plate between all these substances. The reaction always just seems to be a gray spot.
Are there also reagents which can discriminate them on the TLC plate? For as it seems the reagents don't have a problem when not used on the TLC plate. But there I then only get a mix of all colors which is useless on an extract.
It seems they are quite close together (5-MeO-DMT and DMT), but probably they can be differentiated. Anyone any experience if this is the case with the testkit (can one differentiate the spots)?
E.g. How long are the TLC-Plates? And how high would caffeine be, when the liquid reaches the top?
If they are silica, then I could try to use them with Acetic Acid if I wanted a better discrimination between DMT and 5-MeO-DMT.
I had a look in the database endlessness linked in the thread you mentioned. But as it seems the delivered reagents cannot discriminate on the TLC plate between all these substances. The reaction always just seems to be a gray spot.
Are there also reagents which can discriminate them on the TLC plate? For as it seems the reagents don't have a problem when not used on the TLC plate. But there I then only get a mix of all colors which is useless on an extract.
Did you watch the videos that appear when you click on the pictures? There is more to see than a gray spot.Aum_Shanti said:
AFAIK the reagents should work the same whether on a silica plate or on a dish.Aum_Shanti said:
Perhaps endlessness can give you more in depth and more satisfying answers.
Aum_Shanti
Esteemed member
Lol, ah sorry. Didn't realize that these are all videos.
I just saw a video symbol for the 5-MeO-DMT ones. But now I realized that this symbol means, that these videos are not longer available, whereas all the other ones are still working videos.
So thanks for pointing that out. Will order some kit now, as it seems...
I just saw a video symbol for the 5-MeO-DMT ones. But now I realized that this symbol means, that these videos are not longer available, whereas all the other ones are still working videos.
So thanks for pointing that out. Will order some kit now, as it seems...
Ehrlich and hofmann (pdmab-ts) are good to differentiate between dmt and 5-meo-dmt. Here:
If there's too little you might have a hard time seeing on the plate, and if there's too much, the nearby spots may at least partially overlap making diferentiation between them harder. So play around with concentration of samples.
As for the tlc calculator, and the excel calculator linked here in the nexus, they use methanol:25%ammonia (100:2.5) as eluent, which is what is sold with the bunkpolice kit. You can see the 5-meo-dmt and dmt as separate spots, though they are close. You could make another eluent on your own and play around to get better separation, but then the calculator wont work and you'll need your own standards.
If there's too little you might have a hard time seeing on the plate, and if there's too much, the nearby spots may at least partially overlap making diferentiation between them harder. So play around with concentration of samples.
As for the tlc calculator, and the excel calculator linked here in the nexus, they use methanol:25%ammonia (100:2.5) as eluent, which is what is sold with the bunkpolice kit. You can see the 5-meo-dmt and dmt as separate spots, though they are close. You could make another eluent on your own and play around to get better separation, but then the calculator wont work and you'll need your own standards.
Aum_Shanti
Esteemed member
OK, thanks a lot for pointing that out.
Will happily look forward to make TLCs of my plants...
Will happily look forward to make TLCs of my plants...
intosamadhi
Rising Star
Can these kits be used to test for THH ? I see harmine and harmaline in the spot test videos but no THH. Any tips on what is needed (which reagants) for this ?
intosamadhi said:
THH will show up as a faint blue spot under UV below that of harmaline on TLC
JAi said:
It is pretty reliable if you have controls to test against, but for testing complete unknowns, it won't tell you much
But guessing based on length of travel isn't bad either, for this it is best to use the combination of TLC and reagent testing... I wrote a post on this previously.
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